Physicochemical Properties of Mycoviruses: An Overview

  • R. F. Bozarth
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


To comment on the physicochemical properties of all mycoviruses and viruslike particles (VLPs) isolated from fungi in twenty minutes is a formidable task, for many shapes, sizes, and chemical compositions have been documented or postulated. The task is greatly simplified by the fact that only a few mycoviruses have been well characterized and most of these have common properties. In a recent review of physicochemical properties of mycoviruses, only those from 17 fungus species were included (Bozarth, 1979) since most of those described from other species (Lemke, 1979) are known only from electron microscopic investigations.


Penicillium Chrysogenum Agaricus Bisporus Alfalfa Mosaic Virus Fungal Virus Colletotrichum Lindemuthianum 
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  1. Adler JP (1975) Viruses of yeast. Dev Ind Microbiol 16: 152–157Google Scholar
  2. Adler JP, Mackenzie DW (1972) Intrahyphal localizations of Penicillium stoloniferum viruses by fluorescent antibody. Abstr Annu Meet Am Soc Microbiol, p 68Google Scholar
  3. Adler J, Wood HA, Bozarth RF (1976) Virus-like particles from killer, neutral, and sensitive strains of Saccharomyces cerevisiae. J. Virol 17: 472–476PubMedGoogle Scholar
  4. Albouy MM, Lapierre H, Molin G (1973) Mise en evidence d’un nouveau type de particules dans des hyphes de carpophores d’Agaricus bisporus. C R Acad Sci (Paris) Ser D 276:2805–2807Google Scholar
  5. Almond MR, Buck KW, Rawlinson CJ (1977) Viruses of Gaeumannomyces graminis var. tritici. Abstr 2nd Int Mycol Congr (Tampa), p 104Google Scholar
  6. Banks GT, Buck KW, Chain EB, Himmelweit F, Marks JE, Tyler JM, Hollings M, Last FT, Stone OM (1968) Viruses in fungi and interferon stimulation. Nature (London) 218: 542–545CrossRefGoogle Scholar
  7. Banks GT, Buck KW, Chain EB, Darbyshire JE, Himmelweit F, Ratti G, Sharpe TJ, Planterose DN (1970) Antiviral activity of double-stranded RNA from a virus isolated from Aspergillus foetidus. Nature (London) 227: 505–507CrossRefGoogle Scholar
  8. Berry EA, Bevan EA (1972) A new species of double stranded RNA from yeast. Nature (London) 239: 279–280CrossRefGoogle Scholar
  9. Bevan EA, Herring AJ, Mitchell DJ (1973) Preliminary characterization of two species of dsRNA in yeast and their relationship to killer character. Nature New Biol (London) 458: 81–86CrossRefGoogle Scholar
  10. Blattny C, Kralik O, Veselsky J, Kasala B, Herzova H (1973) Particles resembling virions accompanying the proliferation of agaric mushrooms. Ceská Mykol 27: 1–5Google Scholar
  11. Bozarth RF (1977) Biophysical and biochemical characterization of viruslike particles containing a high molecular weight ds-RNA from Helminthosporium maydis. Virology 80: 149–157PubMedCrossRefGoogle Scholar
  12. Bozarth RF (1979) The physico-chemical properties of dsRNA mycoviruses. In: Viruses and Plasmids in fungi. Lemke PA (ed). Marcel Dekker Inc, New York, in pressGoogle Scholar
  13. Bozarth RF, Goenaga A (1977) A complex of virus-like particles from Thielaviopsis basicola. J Virol 24: 846–849PubMedGoogle Scholar
  14. Bozarth RF, Harley EH (1976) The electrophoretic mobility of double-stranded RNA in Polyacrylamide gels as a function of molecular weight. Biochim Biophys Acta 432: 329–335PubMedGoogle Scholar
  15. Bozarth RF, Lentz ET (1978) Physico-chemical properties and serology of Ustilago maydis viruses PI and P4. Abstr 4th Int Congr Virol (The Hague, The Netherlands), p 172Google Scholar
  16. Bozarth RF, Wood HA (1970) Purification and properties of two viruses from Penicillium stoloni-ferum. Phytopathology (Abstr) 60: 1285Google Scholar
  17. Bozarth RF, Wood HA, Mandelbrot A (1971) The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology 45: 516–523PubMedCrossRefGoogle Scholar
  18. Bozarth RF, Wood HA, Goenaga A (1972) Virus-like particles from a culture of Diplocarpon rosae. Phytopathology (Abstr) 62: 493CrossRefGoogle Scholar
  19. Buck KW, Girvan RF (1977) Comparison of the biophysical and biochemical properties of Penicillium cyaneo-fulvum virus and Penicillium chrysogenum virus. J Gen Virol 34: 145–154PubMedCrossRefGoogle Scholar
  20. Buck KW, Kempson-Jones GF (1970) Three types of virus particles in Penicillium stoloniferum. Nature (London) 225: 945–946CrossRefGoogle Scholar
  21. Buck KW, Kempson-Jones, GF (1973) Biophysical properties of Penicillium stoloniferum virus S. J Gen Virol 18: 223–235PubMedCrossRefGoogle Scholar
  22. Buck KW, Kempson-Jones GF (1974) Capsid polypeptides of two viruses isolated from Penicillium stoloniferum. J Gen Virol 22: 441–445CrossRefGoogle Scholar
  23. Buck KW, Ratti G (1975) Biophysical and biochemical properties of two viruses isolated from Aspergillus foetidus. J Gen Virol 27: 211–224PubMedCrossRefGoogle Scholar
  24. Buck KW, Ratti G (1977) Molecular weight of double-stranded RNA: a re-examination of Aspergillus foetidus virus S RNA components. J Gen Virol 37: 215–219PubMedCrossRefGoogle Scholar
  25. Buck KW, Girvan RF, Ratti G (1973a) Two serologically distinct double-stranded ribonucleic acid viruses isolated horn Aspergillus niger. Biochem Soc Trans 1: 1138–1140Google Scholar
  26. Buck KW, Lhoas P, Border DJ, Street BK (1973b) Virus particles in yeast. Biochem Soc Trans 1: 1141–1142Google Scholar
  27. Burnett JP, Frank BH, Douthart RJ (1975) Ionic-strength effects on stability and conformation of Penicillium chrysogenum mycophage double-stranded RNA. Nucleic Acid Res 2: 759–771PubMedCrossRefGoogle Scholar
  28. Day PR, Dodds JA, Elliston JE, Jaynes RA, Anagnostakis SL (1977) Double-stranded RNA in Endothia parasitica. Phytopathology 67: 1393–1396CrossRefGoogle Scholar
  29. Dieleman-van Zaayen A (1967) Virus-like particles in a weed mould growing on mushroom trays. Nature (London) 216: 595–596CrossRefGoogle Scholar
  30. Dieleman-van Zaayen A, Igesz O, Finch JT (1970) Intracellular appearance and some morphological features of virus-like particles in an ascomycete fungus. Virology 42: 534–537CrossRefGoogle Scholar
  31. Dunkle LD (1974) Double-stranded RNA mycovirus inPericonia circinata. Physiol Plant Pathol 4: 107–116CrossRefGoogle Scholar
  32. Ellis LF, Kleinschmidt WJ (1967) Virus-like particles of a fraction of statolon, a mould product. Nature (London) 215: 649–650CrossRefGoogle Scholar
  33. Herring AJ, Bevan EA (1974) Virus-like particles associated with the double-stranded-RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae. J Gen Virol 22: 387–394PubMedCrossRefGoogle Scholar
  34. Hollings M (1962) Viruses associated with a die-back disease of cultivated mushroom. Nature (London) 196: 962–965CrossRefGoogle Scholar
  35. Hollings M (1978) Mycoviruses: viruses that infect fungi. In: Lauffer MA, Bang FB, Maramorosch K, Smith KM (eds) Advances in virus research, vol 22. Academic Press, New York, pp 1–53CrossRefGoogle Scholar
  36. Inoue T (1970) Virus-like particles in L. edodes. Mushrooms 2: 18–22Google Scholar
  37. Kaper JM, Diaz-Ruiz JR (1977) Molecular weights of the double-stranded RNAs of cucumber mosaic virus strain S and its associated RNA 5. Virology 80: 214–217PubMedCrossRefGoogle Scholar
  38. Kazama FY, Schornstein KL (1972) Herpes-type virus particles associated with a fungus. Science 177: 696–697PubMedCrossRefGoogle Scholar
  39. Kazama FY, Schornstein KL (1973) Ultrastructure of a fungus herpes-type virus. Virology 52: 478–487PubMedCrossRefGoogle Scholar
  40. Khandjian EW, Turian G, Eisen H (1977) Characterization of the RNA mycovirus infecting Allomyces arbuscula. J Gen Virol 35: 415–424CrossRefGoogle Scholar
  41. Koltin Y (1978) Virus-like particles in Ustilago maydis: mutants with partial genomes. Genetics 86: 527–534Google Scholar
  42. Koltin Y, Day PR (1976a) Inheritance of killer phenotypes and double-stranded RNA in Ustilago maydis. Proc Natl Acad Sci USA 73: 594–598PubMedCrossRefGoogle Scholar
  43. Koltin Y, Day PR (1976b) Suppression of the killer phenotype in Ustilago maydis. Genetics 82: 629–637PubMedGoogle Scholar
  44. Koltin Y, Berick R, Stamberg J, Ben-Shaul Y (1973) Virus-like particles and cytoplasmic inheritance of plaques in a higher fungus. Nature New Biol 241: 108–109PubMedGoogle Scholar
  45. Kiintzel H, Barath Z, Ali I, Kind J, Althaus NH (1973) Virus-like particles in an extranuclear mutant of Neurospora crassa. Proc Natl Acad Sci USA 70: 1574–1578CrossRefGoogle Scholar
  46. Lampson GP, Tytell AA, Field AK, Nemes MM, Hilleman MR (1967) Inducers of interferon and host resistance. 1. Double-stranded RNA from extracts of Penicillium funiculosum. Proc Natl Acad Sci USA 58: 782–789PubMedCrossRefGoogle Scholar
  47. Lapierre H, Faivre-Amiot A, Kusiak C, Molin G (1972) Particules de type viral associées au Mycogone perniciosa Magnus, agent d’une des môles du champignon de couche. C R Acad Sci (Paris) Ser D 274: 1867–1870Google Scholar
  48. Lemke PA (ed) (1979) Viruses and plasmids in fungi. Marcel Dekker Inc, New York, in pressGoogle Scholar
  49. Lentz E (1977) Physical, molecular, and serological characteristics of the virus-like particles of the corn smut fungus: Ustilago maydis. M A Thesis, Indiana State UniversityGoogle Scholar
  50. Lesemann DE, Koenig R (1977) Association of clubshaped virus-like particles with a severe disease of Agaricus bisporus. Phytopathol Z 89: 161–169CrossRefGoogle Scholar
  51. Loening UE, Ingle J (1967) Diversity of RNA components in green plant tissues. Nature (London) 215: 363–367CrossRefGoogle Scholar
  52. Marino R, Saksena KN, Schuler M, Mayfield JE, Lemke PA (1976) Double-stranded RNA from Agaricus bisporus. Appl Environ Microbiol 31: 433–438PubMedGoogle Scholar
  53. Milne RG, Luisoni E (1977) Rapid immune electron microscopy of virus preparations. In: Maramorosch K, Koprowski H (eds) Methods in virology, vol 6. Academic Press, New York, and London, pp 265–281Google Scholar
  54. Molin G, Lapierre H (1973) L’acide nucléique des virus de champignons: cas des virus de l’Agaricus bisporus. Ann Phytopathol 5: 233–240Google Scholar
  55. Moyer JW, Smith SH (1976) Partial purification and antiserum production to the 19 X 50-nm mushroom virus particle. Phytopathology 66: 1260–1261CrossRefGoogle Scholar
  56. Pollak A (1975) The induction of virus-like particles in the marine phycomycete, Thraustochytrium aureum. Abstr Annu Meet Am Soc Microbiol, p 190Google Scholar
  57. Ratti G, Buck KW (1972) Vitus particles in Aspergillus foetidus: a multicomponent system. J Gen Virol 14: 165–175CrossRefGoogle Scholar
  58. Rawlinson CJ, Hornby D, Pearson V, Carpenter JM (1973) Virus-like particles in the take-all fungus, Gaeumannomyces graminis. Ann Appl Biol 74: 197–209CrossRefGoogle Scholar
  59. Rawlinson CJ, Carpenter JM, Muthyalu G (1975) Double-stranded RNA virus in Colletotrichum lindemuthianum. Trans.Br Mycol Soc 65: 305–341CrossRefGoogle Scholar
  60. Sanderlin RS, Ghabrial SA (1978) Physicochemical properties of two distinct types of virus-like particles from Helminthosporium victoriae. Virology 87: 142–151PubMedCrossRefGoogle Scholar
  61. Schnepf E, Soeder CJ, Hegewald E (1970) Polyhedral virus-like particles lysing the aquatic phycorny cete Aphelidium sp., a parasite of the green alga Scenedesmus alatus. Virology 42: 482–487PubMedCrossRefGoogle Scholar
  62. Schnepf E, Hegewald E, Soeder CJ (1971) Elektronenmikroskopische Beobachtungen an Parasiten aus Scenedesmus- Massenkulturen. 2. Über Entwicklung und Parasit-Wirt Kontakt von Aphelidium und virusartige Partikel im Cytoplasma infizierter Scenedesmus Zellen. Arch Microbiol 75: 209–299CrossRefGoogle Scholar
  63. Semancik JS, Vidaver AK, Van Etten JL (1973) Characterization of a segmented double-helical RNA from bacteriophage φ6. J Mol Biol 78: 617–625PubMedCrossRefGoogle Scholar
  64. Shatkin AJ, Sipe JD, Loh P (1968) Separation of ten reovirus genome segments by Polyacrylamide gel electrophoresis. J Virol 2: 986–991PubMedGoogle Scholar
  65. Tikhonenko TI (1978) Viruses of fungi capable of replication in bacteria (PB viruses). In: Fraen-kel-Conrat HF, Wagner RR (eds) Comprehensive virology, vol 12. Plenum Press, New York, pp 235–269Google Scholar
  66. Tuveson RW, Peterson JF (1972) Virus-like particles in certain slow-growing strains of Neurospora crassa. Virology 47: 527–531PubMedCrossRefGoogle Scholar
  67. Tuveson RW, Sargent ML, Bozarth RF (1975) Purification of a small virus-like particle from strains of Neurospora crassa. Abstr Annu Meet Am Soc Microbiol, p 216Google Scholar
  68. Vodkin MH, Fink GR (1973) A nucleic acid associated with a killer strain of yeast. Proc Natl Acad Sci USA 70: 1069–1072PubMedCrossRefGoogle Scholar
  69. Volkoff O, Walters T (1970) Virus-like particles in abnormal cells of Saccharomyces carlsbergensis. Can J Genet Cytol 12: 621–626PubMedGoogle Scholar
  70. Wickner RB (1976) Killer of Saccharomyces cerevisiae: a double-stranded ribonucleic acid plasmid. Bacteriol Rev 40: 757–773PubMedGoogle Scholar
  71. Wood HA, Bozarth RF (1972) Properties of viruslike particles oîPenicillium chrysogenum: one double-stranded RNA molecule per particle. Virology 47: 604–609PubMedCrossRefGoogle Scholar
  72. Wood HA, Bozarth RF (1973) Heterokaryon transfer of viruslike particles associated with a cyto-plasmically inherited determinant in Ustilago maydis. Phytopathology 63: 1019–1021CrossRefGoogle Scholar
  73. Wood HA, Bozarth RF, Mislivec PB (1971) Viruslike particles associated with an isolate of Penicillium brevi-compactum. Virology 44: 592–598PubMedCrossRefGoogle Scholar
  74. Wood HA, Bozarth RF, Adler J, Mackenzie DW (1974) Proteinaceous virus-like particles from an isolate of Aspergillus flavus. J.Virol 13:532–534PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • R. F. Bozarth
    • 1
  1. 1.Department of Life SciencesIndiana State UniversityTerre HauteUSA

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