Skip to main content

Separation and Isolation of H and L Chains of IgG

  • Chapter
  • 794 Accesses

Abstract

Reductive cleavage of the -S-S- bonds in an IgG molecule will liberate the H and L chains. If this cleavage is carried out near neutral pH and with mild reducing agents such as dithio-threitol, no further degradation and denaturation of the split products will occur. To block the sulfhydryl groups set free by the reductive cleavage, alkylation with iodoacetamide has to be carried out. The H and L chains can be separated by gel electrophoresis in 8 M urea-formic acid buffer, or by column chromatography on Sephadex G 100 equilibrated with 8 M urea in pH 6 Triscitrate buffer.

Keywords

  • Antibody Fragment
  • Reductive Cleavage
  • Split Product
  • Drop Counter
  • Insoluble Matrix

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, access via your institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-3-642-67356-6_7
  • Chapter length: 3 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   109.00
Price excludes VAT (USA)
  • ISBN: 978-3-642-67356-6
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   139.00
Price excludes VAT (USA)

References

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

Copyright information

© 1979 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Nowotny, A. (1979). Separation and Isolation of H and L Chains of IgG. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-67356-6_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-09453-1

  • Online ISBN: 978-3-642-67356-6

  • eBook Packages: Springer Book Archive