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Enzymatic Cleavage of IgG

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Abstract

In the study of active sites of natural products one of the main goals is to obtain the smallest possible fragments of the macro-molecule while maintaining biologic activity. Such an attempt was made by Porter (1959) who, in his classical work, described the cleavage of rabbit antisera by papain and the Chromatographic fractionation of the fragments obtained. In the present exercise the work of Porter will be reproduced.

Keywords

  • DEAE Cellulose
  • EDTA Buffer
  • Chromatographic Fractionation
  • Serologic Reactivity
  • Present Exercise

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© 1979 Springer-Verlag Berlin Heidelberg

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Nowotny, A. (1979). Enzymatic Cleavage of IgG. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_5

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  • DOI: https://doi.org/10.1007/978-3-642-67356-6_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-09453-1

  • Online ISBN: 978-3-642-67356-6

  • eBook Packages: Springer Book Archive