Skip to main content
SpringerLink
Log in
Book cover

Basic Exercises in Immunochemistry pp 84–86Cite as

A and B Blood Group Antigen Preparations From Erythrocytes

  • Chapter

  • 925 Accesses

Abstract

The ABO group antigens of human red bloods cells (RBC) are localized in the stroma (synonyms are membrane or ghost) of the cells. Hemolysis of the RBC is carried out in distilled water and subsequent repeated washing of the insoluble residue leads to a stroma preparation which is still light red from the presence of remnants of hemoglobin. It was observed that dilute alkali treatment appears to solubilize the prepared stroma without changing the reactivity of A or B or AB antigens with isoagglutinins. Subsequent careful neutralization of this alkaline preparation keeps the stroma solubilized (Nowotny, 1955). This is described in Part A of this exercise.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Abdelnoor, A., Higgins, M., Nowotny, A.: In: Cellular Antigens, p. 153 Nowotny, A. (ed.) Berlin-Heidelberg-New York: Springer 1971

    Google Scholar 

  • Hakomori, S.-I., Jeanloz, R.W.: J. Biol. Chem. 236, 2827 (1961)

    PubMed  CAS  Google Scholar 

  • Hakomori, S.-I., Kobata, A.: In: The Antigens, Sela, M. (ed.) London, New York: Acadmic Press, vol. II., p. 79, 1974

    Google Scholar 

  • Hakomori, S.-I., Stycharz, G.D.: Biochemistry, 7, 1279 (1968)

    Article  PubMed  CAS  Google Scholar 

  • Koscielak, J.: Biochim. Biophys. Acta 78, 313 (1963)

    Article  PubMed  CAS  Google Scholar 

  • Nowotny, A.: Acta Physiol. Acad. Sci. Hung. 7, 31 (1955)

    CAS  Google Scholar 

  • Nowotny, A. (ed.): In: Cellular Antigens, p. 161, Berlin-Heidelberg-New York: Springer 1971

    Google Scholar 

  • Nowotny, A., O’Neill, G.J.: Lecture, Xth Congress Int. Soc. Hematol. Stockholm, 1964

    Google Scholar 

  • Yamakawa, T., Suzuki, S.: J. Biochem. 39, 393 (1952)

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University of Pennsylvania, 4001 Spruce Street, Philadelphia, PA, 19104, USA

    Alois Nowotny (Professor of Immunology)

Authors
  1. Alois Nowotny
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1979 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Nowotny, A. (1979). A and B Blood Group Antigen Preparations From Erythrocytes. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_27

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-67356-6_27

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-09453-1

  • Online ISBN: 978-3-642-67356-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation