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Preparation of Soluble Dinitrophenyl Proteins

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Abstract

The method described in Exercise No, 44 yields an insoluble dinitrophenyl (DNP) protein which is suitable for chemical analysis, but cannot be used for immunobiologic assays. Eisen (1964) prepared DNP protein derivatives which are readily soluble in water or saline using a mild procedure. Using dinitrophenyl sulfonate instead of dinitrofluorobenzene, more selective dinitrophenylation could be achieved. This reagent is water soluble; its removal requires simple dialysis (or gel filtration). The number of DNP groups introduced on a protein can be measured photometrically.

Keywords

  • Human Serum Albumin
  • Potassium Carbonate
  • Intense Yellow Color
  • Lysine Derivative
  • Mild Procedure

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© 1979 Springer-Verlag Berlin Heidelberg

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Nowotny, A. (1979). Preparation of Soluble Dinitrophenyl Proteins. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_15

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  • DOI: https://doi.org/10.1007/978-3-642-67356-6_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-09453-1

  • Online ISBN: 978-3-642-67356-6

  • eBook Packages: Springer Book Archive