Abstract
A major disadvantage of the earlier protein iodination procedures lies in the use of some harsh chemicals which can introduce unwanted and irreversible changes in the structure of the labeled preparations. To circumvent this, iodination by peroxidase enzyme-containing or peroxide generating systems was applied by numerous authors since the first short report on the feasibility of this by Keston (1944), It has been used for Immunoglobulin labeling by Marchalonis (1969) using lactoperoxidase, hydrogen peroxide, and iodide. The procedure described here in Part A is based on this procedure. Part B describes the procedure used by Dr. Ah-kau Ng (NIH, National Cancer Institute, Bethesda, MD) to iodinate exposed proteins on human tumor cell surfaces, based on the publication of Phillips and Morrison (1971).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Cone, R.E., Marchalonis, J.J.: Biochem. J. 140, 345 (1974)
David, G.S., Reisfeld, R.A.: Biochem. 13, 1014 (1974)
Henning, R., Milner, R.J., Reske, K., Cunningham, B.A., Edelman, G.M.: Proc. Natl. Acad. Sci. USA 73, 118 (1976)
Keston, A.S.: J. Biol. Chem. 153, 335 (1944)
Marchalonis, J.J.: Biochem. J. 113, 299 (1969)
Phillips, D.R., Morrison, M.: Biochem. 10, 1766 (1971)
Snyder, H.W., Jr., Stockert, E., Fleissner, E.: J, Virol, 23, 302 (1977)
Taylor, C.: The Lancet, Oct. 5, p 802, (1974)
Vitetta, E.S., Baur, S., Uhr, J.W.: J. Exp. Med, 134, 242 (1971)
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1979 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Nowotny, A. (1979). Enzymatic Labeling of Proteins with 125I. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_13
Download citation
DOI: https://doi.org/10.1007/978-3-642-67356-6_13
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-09453-1
Online ISBN: 978-3-642-67356-6
eBook Packages: Springer Book Archive