Conjugation of Antibodies with Fluorescein and Rhodamine
Labeled antibodies provide one of the most indispensable tools for the localization of antigens in tissue sections, in smears, or in microbial preparations. The rather high specificity of the antigen-antibody reaction permits discovering antigens in situ without isolating them from the surrounding components. For these purposes, the antibody produced in vivo against those sought for antigenic macromolecules has to be labeled with a tracer.
KeywordsLabel Antibody Nonspecific Staining Sephadex Column Microscopic Preparation Microbial Preparation
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