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Conjugation of Antibodies with Fluorescein and Rhodamine

  • Alois Nowotny

Abstract

Labeled antibodies provide one of the most indispensable tools for the localization of antigens in tissue sections, in smears, or in microbial preparations. The rather high specificity of the antigen-antibody reaction permits discovering antigens in situ without isolating them from the surrounding components. For these purposes, the antibody produced in vivo against those sought for antigenic macromolecules has to be labeled with a tracer.

Keywords

Label Antibody Nonspecific Staining Sephadex Column Microscopic Preparation Microbial Preparation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Alois Nowotny
    • 1
  1. 1.University of PennsylvaniaPhiladelphiaUSA

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