Abstract
Labeled antibodies provide one of the most indispensable tools for the localization of antigens in tissue sections, in smears, or in microbial preparations. The rather high specificity of the antigen-antibody reaction permits discovering antigens in situ without isolating them from the surrounding components. For these purposes, the antibody produced in vivo against those sought for antigenic macromolecules has to be labeled with a tracer.
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References
Cherry, W.B.: Personal communication (1966)
Coons, A.H., Creech, N.J., Jones, R.N.: Proc. Soc. Exp. Biol. Med. 47, 200 (1941)
Coons, A.H., Kaplan, M.H.: J. Exp. Med. 91, 1 (1950)
Hokenson, E.O., Hansen, P.A.: Stain technology 41, 9 (1966)
Killander, J., Ponten, J., Roden, L.: Nature London 192, 182 (1961)
Lewis, V.J., Jones, W.L., Brooks, J.B., Cherry, W.B.: J. Appl. Microbiol. 12, 343 (1964)
McKinney, R.M., Spillane, J.T., Pearce, G.W.: J. Immunol. 93, 232 (1964)
Nairn, R.C.: Fluorescent protein tracing. Baltimore, Md.: Williams & Wilkins Co. 1964
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© 1979 Springer-Verlag Berlin Heidelberg
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Nowotny, A. (1979). Conjugation of Antibodies with Fluorescein and Rhodamine. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_12
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DOI: https://doi.org/10.1007/978-3-642-67356-6_12
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-09453-1
Online ISBN: 978-3-642-67356-6
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