Summary
Immunization of prospective bone marrow recipients with thymocytes of the marrow donor strain prevented subsequent hemopoietic engraftment of the marrow. Immunization with thymocytes of a third party strain sharing the marrow donor’s theta alloantigen but not his histocompatibility antigens permitted chimaerism and suppressed GVH in mice.
Bone marrow of rat strains of the Th-1.1 group did not take in mice sensitized against mouse Th-1.1 thymocytes. This observation lead to the conclusion that hemopoietic stem cells of rats but not of mice express an early thymic and even prethymic antigen. This antigen is not restricted to the Th-1.1 specificity: rabbit anti-rat thymocyte globulin absorbed in a way which removed stem cell toxicity from a rabbit anti-mouse thymocyte globulin prevented hemopoietic engraftment when incubated with rat bone marrow before transfer to irradiated mice. Serologically this antiserum reacted with thymocytes as well as lymphocytes in the rat bone marrow but less with lymphnode cells. An antiserum against rat lymphnode cells was rendered T cell specific by absorption. It defined a postthymic T antigen lacking on stem cells, bone marrow lymphocytes and thymus. It labeled lymphocytes in the interfollicular T dependent area of lymphnodes by an immunohistochemical method. T cells in rats were thus found to express a prethymic and/or a postthymic antigen.
Implications of theta alloantigens for the suppression of GVH in polytransfused bone marrow grafted patients are discussed.
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References
Rodt, H. V., Thierfelder, S., Eulitz, M.: Suppression of acute secondary disease by heterologous anti-brain serum. Blut 25, 385–389 (1972)
Rodt, H., Thierfelder, S., Eulitz, M.: Anti-lymphocytic antibodies and marrow transplantation. III. Effect of heterologous anti-brain antibodies on acute secondary disease in mice. Eur. J. Immunol. 4, 25–29 (1974)
Thierfelder, S., Rodt, H.: Antilymphocytic antibodies and marrow transplantation. V. Suppression of secondary disease by host-versus-O-graft-reaction. Transplantation 23, 87–92 (1977)
Acton, R. T., Blankenhorn, E. P., Douglas, T. C, Owen, R. D., Hilgers, J., Hoffmann, H. A., Boyse, E. A.: AKR-mice-genetic variations among sublines. Nature 245, 8–10 (1973)
Douglas, T. C.: Occurrence of a theta-like antigen in rats. J. Exp. Med. 136, 1054–1062 (1972)
Micheel, B., Pasternak, G., Steuden, J.: Demonstration of 0-AKR differentiation antigen in rat tissue by mouse alloantiserum. Nature 241, 221–222 (1973)
Vos, O., Davids, J. A. G., Weyzen, W. W. H., Bekkum, D. W. van: Evidence for the cellular hypothesis in radiation protection by bone marrow cells. Acta physiol. pharmacol. 4, 482–486 (1956)
Ford, C. E.: The use of marker chromosomes. In: Micklem, H. S., Contit, J. F.: Tissue grafting and radiation, p. 197. New York: Academic Press 1966
Hoffmann-Fezer, G., Rodt, H., Eulitz, M., Thierfelder, S.: Immunohistochemical identification of T-and B-lymphocytes delineated by the unlabeled antibody enzyme method. I. Anatomical distribution of 0-positive and Ig-positive cells in lymphoid organs of mice. J. Immunol. Methods 13, 261–270 (1976)
Sternberger L. A., Cuculis: Method for enzymatic intensification of the immunocytochemical reaction without use of labelled antibodies. J. Histochem. Cytochem. 17, 190 (1969)
Storb, R., Epstein, R. B., Rudolph, R. H., Thomas, E. D.: The effect of prior transfusion on marrow grafts between histocompatible canine siblings. J. Immunol. 105, 627–633 (1970)
Thierfelder, S.: Haemopoietic stem cells of rats but not of mice express Th-1.1 alloantigen. Nature 269, 691–693 (1977)
Hunt, S. V., Mason, D. W., Williams, A. F.: Rat bone marrow Thy-1 antigen is present on cells with membrane immunoglobulin and on precursors of peripheral B-lymphocytes. Eur. J. Immunol. 7, 11, 817–823 (1977)
Goldschneider, I.: Antigenic relationship between medullary thymocytes and a subpopulation of peripheral T cells in the rat: Description of a masked antigen. Cell. Immunol. 16, 269–284 (1975)
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Thierfelder, S., Thiel, E., Hoffmann-Fezer, G., Rodt, H. (1980). Bone Marrow Transplantation into Recipients Sensitized Against Donor-Type T Cells. In: Thierfelder, S., Rodt, H., Kolb, H.J. (eds) Immunobiology of Bone Marrow Transplantation. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 25. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67319-1_18
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DOI: https://doi.org/10.1007/978-3-642-67319-1_18
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