2-Hydroxyoestradiol and (+)-Cyanidanol-3 Prevent Lipid Peroxidation of Isolated Rat Hepatocytes
Isolated rat hepatocytes were aerobically incubated in absence or in presence of 2 mM bromotrichloromethane (CBrCl3). Viability criteria, such as trypan blue uptake and lactate dehydrogenase release of the cells increased with incubation time in both systems. An increase in malondialdehyde (MDA) formation of the cells was observable in both the CBrCl3-containing and the CBrCl3-free incubation mixtures.
The addition of 1 × 10-4 M 2-hydroxyoestradiol (2-OH-E2) as well as of 1 × 10-4 M (+)-cyanidanol-3 (C-3) totally prevented the trypan blue uptake, the lactate dehydrogenase release, as well as the MD A formation of the hepatocytes incubated without CBrCl3. When the hepatocytes were incubated with 2 mM CBrCl3, the observed considerable increase in the number of cells taking up trypan blue, releasing lactate dehydrogenase or forming MDA decreased significantly by adding 1 × 10-4 M 2-OH-E2 or C-3 respectively to the incubation mixture. A concentration of 1.8 × 10-5 M 2-OH-E2 inhibited the CBrCl3-in-duced MDA formation to 50%, the corresponding value being 9.1 × 10-5 M for C-3.
The results indicate that during incubation of isolated hepatocytes lipid peroxidation and cell damage does occur with and without CBrCl3, and that lipid peroxidation in hepatocytes as well as cell damage can be prevented by agents well known to inhibit lipid peroxidation in isolated hepatic microsomes.
Key words2-Hydroxyoestradiol (+)-Cyanidanol-3 CBrCl3 Lipid Peroxidation Isolated Hepatocytes
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