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Red Cell Deformability Changes in Hemolytic Anemias Estimated by Diffractometric Methods (Ektacytometry) Preliminary Results

  • C. Allard
  • N. Mohandas
  • M. Bessis

Abstract

Red cell deformability changes have been evaluated in several types of hemolytic anemias using a new diffractometric technique (ektacytometer). Varying proportions (10–30%) of undeformable cells were found in patients with hereditary spherocytosis. Significantly higher proportions (60–80%) of undeformable cells were detected in patients with hemoglobin C-C and unstable hemoglobin disorders. Patients with G6PD deficiency had near normal deformabilities, while patients with PK deficiency had small subpopulations (10–15%) of undeformable cells. These results confirm the earlier reports of reduced deformability of red cells in various hemolytic anemias and provide additional quantitative data on the presence of subpopulations of undeformable cells. Our technique requires small quantities of blood (50 μl) and provides easily interpretable data on the deformability characteristics of the whole population of cells.

Key Words

Red cell deformability Hemolytic anemias Hereditary spherocytosis Hemoglobinopathies Laser diffractometry 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1978

Authors and Affiliations

  • C. Allard
    • 1
  • N. Mohandas
    • 2
  • M. Bessis
    • 1
  1. 1.Institut de Pathologie Cellulaire (Inserm, U. 48)Hôpital de BicêtreKremlin BicêtreFrance
  2. 2.Cancer Research InstituteUniversity of CaliforniaSan FranciscoUSA

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