Red Cell Deformability Changes in Hemolytic Anemias Estimated by Diffractometric Methods (Ektacytometry) Preliminary Results
Red cell deformability changes have been evaluated in several types of hemolytic anemias using a new diffractometric technique (ektacytometer). Varying proportions (10–30%) of undeformable cells were found in patients with hereditary spherocytosis. Significantly higher proportions (60–80%) of undeformable cells were detected in patients with hemoglobin C-C and unstable hemoglobin disorders. Patients with G6PD deficiency had near normal deformabilities, while patients with PK deficiency had small subpopulations (10–15%) of undeformable cells. These results confirm the earlier reports of reduced deformability of red cells in various hemolytic anemias and provide additional quantitative data on the presence of subpopulations of undeformable cells. Our technique requires small quantities of blood (50 μl) and provides easily interpretable data on the deformability characteristics of the whole population of cells.
Key WordsRed cell deformability Hemolytic anemias Hereditary spherocytosis Hemoglobinopathies Laser diffractometry
Unable to display preview. Download preview PDF.
- 1.Allard, C., Mohandas, N., Wacjman, H., Krisnnamoorthy, R.: A case of great instability of the hemoglobin: Hemoglobin Bicêtre. Nouv. Rev. fr. Hémat.16, 23, 1976Google Scholar
- 2.Bessis, M., Mohandas, N.: A diffractometric method for the measurement of cellular deforatability. Blood Cells1, 307, 1975Google Scholar
- 3.Bessis, M., Mohandas, N.: Deformability of normal, shape-altered and pathological red cells. Blood Cells1, 315, 1975Google Scholar
- 4.Jacob, H.S.: Dysfunction of the red cell membrane. In: Surgenor, D.M., ed., The Red Blood Cell, 2nd ed., New York, Academic Press, 1974, p. 269Google Scholar
- 10.Sutera, S.P., Mehrjardi, M., Mohandas, N.: Deformation of erythrocytes under shear. Blood Cells1, 369, 1975Google Scholar