Integration Sites for Moloney Murine Leukemia Virus DNA in Infected Mouse Cells

  • L. T. Bacheler
  • H. Fan
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 23)


The integration of viral DNA in mouse cells infected with Moloney murine leukemia virus (M-MuLV) has been studied by the “blotting” technology introduced by Southern (1975). We were interested in determining if there is a single site in the chromosomal DNA at which M-MuLV viral DNA is integrated, a small number of sites, or a large number of possible integration sites. The technique used involved cleavage of infected cell DNA with a sequence-specific restriction endonuclease, resolution of the resulting DNA fragments by electrophoresis in 0.6% agarose gels, and blot transfer of the separated fragments to nitrocellulose filters. Detection of DNA fragments with sequence homology to M-MuLV was achieved by hybridization with 32P-labeled M-MuLV complementary DNA (cDNA). The labeled cDNA (approximately 2 x 108 cpm/µg) was synthesized in an endogenous reaction using purified virus and added calf thymus oligo-deoxynucleotide primers in order to generate a uniformly representative probe (Fan and Verma, 1978).


Integration Site Mouse Cell Murine Leukemia Virus Moloney Murine Leukemia Virus Infected Cell Line 
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  1. Bacheler, L., Fan, H.: Multiple Integration Sites for Moloney Murine Leukemia Virus in Productively Infected Mouse Fibroblasts. J. of Virology, in press (1979)Google Scholar
  2. Fan, H., Verma, I.: Size analysis and relationship of murine leukemia virus-specific mRNA’s: Evidence for transposition of sequences during synthesis and processing of subgenomic mRNA. J. Virol. 26, 468–478 (1978)PubMedGoogle Scholar
  3. Jaenisch, R.: Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus. Proc. Natl. Acad. Sci. USA 73, 1260–1264 (1976)PubMedCrossRefGoogle Scholar
  4. Jaenisch, R., Fan, H., Croker, B.: Infection of preimplantation mouse embryos and of newborn mice with leukemia virus: Tissue distribution of viral DNA and RNA and leukemogenesis in the adult animal. Proc. Natl. Acad. Sci. USA 72, 4008–4012 (1975)PubMedCrossRefGoogle Scholar
  5. Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)PubMedCrossRefGoogle Scholar
  6. Verma, I.M., McKennett, M.A.: Genome organization of RNA tumor virus. II. Physical maps of in vitro-synthesized Moloney murine leukemia virus double-stranded DNA by restriction endonucleases. J. Virol. 26, 630–645 (1978)PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • L. T. Bacheler
    • 1
  • H. Fan
    • 1
  1. 1.Tumor Virology LaboratoryThe Salk InstituteSan DiegoUSA

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