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Integration Sites for Moloney Murine Leukemia Virus DNA in Infected Mouse Cells

  • L. T. Bacheler
  • H. Fan
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 23)

Abstract

The integration of viral DNA in mouse cells infected with Moloney murine leukemia virus (M-MuLV) has been studied by the “blotting” technology introduced by Southern (1975). We were interested in determining if there is a single site in the chromosomal DNA at which M-MuLV viral DNA is integrated, a small number of sites, or a large number of possible integration sites. The technique used involved cleavage of infected cell DNA with a sequence-specific restriction endonuclease, resolution of the resulting DNA fragments by electrophoresis in 0.6% agarose gels, and blot transfer of the separated fragments to nitrocellulose filters. Detection of DNA fragments with sequence homology to M-MuLV was achieved by hybridization with 32P-labeled M-MuLV complementary DNA (cDNA). The labeled cDNA (approximately 2 x 108 cpm/µg) was synthesized in an endogenous reaction using purified virus and added calf thymus oligo-deoxynucleotide primers in order to generate a uniformly representative probe (Fan and Verma, 1978).

Keywords

Integration Site Mouse Cell Murine Leukemia Virus Moloney Murine Leukemia Virus Infected Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bacheler, L., Fan, H.: Multiple Integration Sites for Moloney Murine Leukemia Virus in Productively Infected Mouse Fibroblasts. J. of Virology, in press (1979)Google Scholar
  2. Fan, H., Verma, I.: Size analysis and relationship of murine leukemia virus-specific mRNA’s: Evidence for transposition of sequences during synthesis and processing of subgenomic mRNA. J. Virol. 26, 468–478 (1978)PubMedGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • L. T. Bacheler
    • 1
  • H. Fan
    • 1
  1. 1.Tumor Virology LaboratoryThe Salk InstituteSan DiegoUSA

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