Leukemic Cell Phenotypes in Man: Relationship to ‘Target’ Cells for Leukemogenesis and Differentiation Linked Gene Expression

  • M. F. Greaves
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 23)


Established cell lines derived from malignant cells are proving to be of considerable value in the investigation of differentiation and controls of gene expression. This is especially evident in the leukemias; the Friend virus-induced erythroleukemia (Harrison, 1977), avian erythroleukemia (see Graf, this book) and myeloid leukemias in rats (Lotem and Sachs, 1974) and a recently described human myeloid line (Collins et al., 1978), all having a maturation arrest which can be reversed by various agents and to some extent controlled by viruses (see papers by Gallo, Ruscetti and Graf in this book).


Acute Myeloid Leukemia Acute Lymphoblastic Leukemia Chronic Myeloid Leukemia Acute Lymphoblastic Leukaemia Terminal Deoxynucleotidyl Transferase 
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  1. Boggs, D. R.: Haematopoietic stem cell therapy in relation to possible lymphoblastic conversion of chronic myeloid leukaemia. Blood 44, 449–453 (1974)PubMedGoogle Scholar
  2. Chessells, J. M., Hardisty, R. M., Rapson, N.T., Greaves, M.F.: Acute lymphoblastic leukaemia in children: classification and prognosis. Lancet 1977 II, 1307–1309Google Scholar
  3. Cline, M.J., Billing, R.: Antigens expressed by human B lymphocytes and myeloid stem cells. J. exp. Med. 146, 1143–1145 (1977)PubMedCrossRefGoogle Scholar
  4. Collins, S.J., Ruscetti, F.W., Gallagher, R. E., Gallo, R.C.: Terminal differentiation of human promyelocytic leukaemia cells induced by dimethyl sulfoxide and other polar compounds. Proc. natl. Acad. Sci. USA 75, 2458–2462 (1978)PubMedCrossRefGoogle Scholar
  5. Ellis, R. B., Rapson, N.T., Patrick, D., Greaves, M.F.: Expression of hexosaminidase isoenzymes in childhood leukaemia. New Engl. J. Med. 298,476–480 (1978)PubMedCrossRefGoogle Scholar
  6. Greaves, M. F.: Clinical applications of cell surface markers. Progr. Haematol. 9, 255–303 (1975)Google Scholar
  7. Greaves, M.F.: Immunodiagnosis of Leukaemia. In: Immunodiagnosis of Cancer. Herberman, R., McIntire, K. (eds.). New York: Marcel Dekker Inc. pp. 542–587 (1979)Google Scholar
  8. Greaves, M.F., Capellaro, D., Brown, G., Revesz, T., Janossy, G.: Analysis of human leukaemic cells using cell surface binding probes and the fluorescence activated cell sorter. In: Modern trends in human leukaemia II. Neth, R., Gallo, R.C., Mannweiler, K., Moloney, W.C. (eds.). pp. 243–260. Munich: J. F. Lehmanns Verlag 1976Google Scholar
  9. Greaves, M.F., Janossy, G.: Patterns of gene expression and the cellular origins of human leukaemias. Biochem. Biophys. Acta. 516. 193–230 (1978)PubMedGoogle Scholar
  10. Greaves, M.F., Janossy, G., Francis, G., Minowada, J.: Membrane phenotypes of human leukaemic cells: clinical correlates and biological implications. Cell Proliferation Series. Clarkson, B., Till, J., Marks, P. (eds.). Vol. 5. pp.823–842 (1978). Cold Spring Harbor Publ.Google Scholar
  11. Greaves, M.F., Janossy, G., Roberts, M., Rapson, N.T., Ellis, R. B., Chessells, J., Lister, T.A., Catovsky, D.: Membrane phenotyping: diagnosis, monitoring and classification of acute ‘lymphoid’ leukaemias. In: Immunological diagnosis of leukaemias and lymphomas. Thierfelder, S., Rodt, H., Thiel, E. (eds.), pp. 61–76. Berlin, Heidelberg, New York: Springer 1977CrossRefGoogle Scholar
  12. Greaves, M.F., Verbi, W., Reeves, B.R., Drysdale, H.C., Jones, L., Sacker, L.S., Samaratunga, I.: Pre-B phenotypes in blast crisis of Ph1 positive CML: evidence for a pluripotential stem cell ‘target’. Leuk. Res. 3 (in press)Google Scholar
  13. Harrison, P.R.: The Biology of the Friend Cell. In: Biochemistry of cell differentiation II. Paul, J. (ed.). Vol. 15, pp. 227–267 (1977)Google Scholar
  14. Hoffbrand, A.V., Ganeshaguru, K., Janossy, G., Greaves. M.F., Catovsky, D., Woodruff, R.K.: Terminal deoxynucleotidyl transferase levels and membrane phenotypes. Lancet 1977 II, 520–523Google Scholar
  15. Janossy, G., Francis, G., Capellaro, D., Goldstone, A., Greaves, M.F.: Cell sorter analysis of leukaemia associated antigens on human myeloid precursors Nature 276, 176-178Google Scholar
  16. Janossy, G., Goldstone, A.H., Capellaro, D., Greaves, M.F., Kulenkampff, J., Pippard, M., Welsh, K.: Differentiation linked expression of p28,33 (Ia-like) structures on human leukaemic cells. Brit. J. Haematol. 37, 391–402 (1977b)CrossRefGoogle Scholar
  17. Janossy, G., Greaves, M.F., Sutherland, R., Durrant, J., Lewis, C.: Comparative analysis of membrane phenotypes in acute lymphoid leukaemia and in lymphoid blast crisis of chronic myeloid leukaemia. Leuk. Res. 1, 289–300 (1977a)CrossRefGoogle Scholar
  18. Janossy, G., Roberts, M., Greaves, M.F.: Target cell in chronic myeloid leukaemia and its relationship to common ALL. Lancet 1976 II, 1057–1061Google Scholar
  19. Janossy, G., Woodruff, R.K., Paxton, A., Greaves, M.F., Capellaro, D., Kirk, B., Innes, E.M., Eden, O. B., Lewis, C., Catovsky, D., Hoffbrand, A.V.: Membrane marker and cell separation studies in Ph1-positive leukaemia. Blood 51, 861–877 (1978b)PubMedGoogle Scholar
  20. Jasmin, C., Joffe, F.S., Klein, B., Kerdiles-Le Bousse, C.: Physiopathology of human and virusinduced murine leukaemias. Cancer Research 36,603–607 (1976)PubMedGoogle Scholar
  21. Kaplan, J., Inoue, S., Offenbreit, M. J.: Myeloid colony-forming cells express human B lymphocyte antigens. Nature 271, 458–459 (1978)PubMedCrossRefGoogle Scholar
  22. Lister, T.A., Roberts, M. M., Brearley, R. L., Woodruff, R. K., Greaves, M.F.: Prognostic significance of cell surface phenotype in adult acute lymphoblastic leukaemia. Clin. Immunol. Immunotherapy (in press)Google Scholar
  23. Lotem, J., Sachs, L.: Different blocks in the differentiation of myeloid leukaemic cells. Proc. natl. Acad. Sci. USA 71, 3507–3511 (1974)PubMedCrossRefGoogle Scholar
  24. McCaffrey, R., Harrison, T.A., Kung, P.C., Parkman, R., Silverstone, A.E., Baltimore, D.: Terminal deoxynucleotidyl transferase in normal and neoplastic hematopoietic cells. In: Modern trends in human leukaemia. Neth, R., Gallo, R.C., Mannweiler, K., Moloney, W.C. (eds.), pp. 503–513. Munich: J. F. Lehmanns Verlag 1976Google Scholar
  25. Minowada, J., Janossy, G., Greaves, M.F., Tsubota, T, Srivastava, B.I.S., Morikawa, S., Tatsumi, E.: Expression of an antigen associated with acute lymphoblastic leukaemia in human leukaemia-lymphoma cell lines. J. Natl. Cancer Inst. 60, 1269–1277 (1978)PubMedGoogle Scholar
  26. Minowada, J., Koshiba, H., Janossy, G., Greaves, M.F., Bollum, F.J.: A Philadelphia chromosome positive human leukaemia cell line (NALM-1) with pre-B characteristics. Leuk. Res. 3 (in press)Google Scholar
  27. Minowada, J., Tsubota, T., Greaves, M. F.: A non-T non-B human leukaemia cell line (NALM-1): establishment of the line and presence of leukaemia associated antigens. J. natl. Cancer Inst. 59, 83–87 (1977)PubMedGoogle Scholar
  28. Mohanakumar, T., Metzgar, R. S., Miller, D. S.: Human leukaemia cell antigens: serologic characterization with xenoantisera. J. natl. Cancer Inst. 52, 1435–1444 (1975)Google Scholar
  29. Mohanakumar, T., Miller, D.S., Metzgar, R.S.: Human acute myelomonocytic leukaemia: serologic studies with simian antisera. Blood 48, 339–350 (1976)PubMedGoogle Scholar
  30. Nowell, P.C.: The clonal evolution of tumor cell populations. Science 194, 23–28 (1976)PubMedCrossRefGoogle Scholar
  31. Preud’homme, J.-L., Klein, M., Labaume, S., Seligmann, M.: Idiotype-bearing and antigen-binding receptors produced by blood T lymphocytes in a case of human myeloma. Eur. J. Immunol. 7, 840–846 (1977)CrossRefGoogle Scholar
  32. Roberts, M., Greaves, M.F.: Maturation linked expression of a myeloid cell surface antigen. Brit. J. Haematol. 38, 439–452 (1978)CrossRefGoogle Scholar
  33. Roberts, M., Greaves, M.F., Janossy, G., Sutherland, R., Pain, C.: Acute lymphoblastic leukaemia (ALL) associated antigen. I. Expression in different haemopoietic malignancies. Leuk. Res. 2, 105–114 (1978)CrossRefGoogle Scholar
  34. Rosenfeld, C., Goutner, A., Choquet, C., Venuat, A.M., Kayibanda, B., Pico, J.L., Greaves, M. F.: Phenotypic characterisation of a unique non-T,non-B acute lymphoblastic leukaemia cell line. Nature 267, 841–843 (1977)PubMedCrossRefGoogle Scholar
  35. Salmon, S.E., Seligmann, M.: B-cell neoplasia in Man. Lancet 1974 II, 1230–1233Google Scholar
  36. Sarin, P. S., Anderson, P., Gallo, R.C.: Terminal deoxynucleotidyl transferase activities in human blood leukocytes and lymphoblast cell lines: high levels in lymphoblast cell lines and in blast cells of some patients with chronic myelogenous leukaemia in acute phase. Blood 47, 11–20 (1976)PubMedGoogle Scholar
  37. Schlossmann, S. F., Chess, L., Humphreys, R. E., Strominger, J.L.: Distribution of Ia-like molecules on the surface of normal and leukaemic cells. Proc. natl. Acad. Sci. USA 73, 1288–1293 (1976)CrossRefGoogle Scholar
  38. Smart, J., Sutherland, R., Greaves. M.F. (in preparation)Google Scholar
  39. Sutherland, R., Smart, J., Niaudet, P., Greaves, M.F.: Acute lymphoblastic leukaemia associated antigen. II. Isolation and partial characterisation. Leuk. Res. 2, 115–126 (1978)CrossRefGoogle Scholar
  40. Thierfelder, S., Rodt, H., Thiel, E. (eds.): Immunological Diagnosis of Leukaemias and Lymphomas. Berlin, Heidelberg. New York: Springer 1977Google Scholar
  41. Vogler, L.B., Crist, W.M., Bockman, D.E., Pearl, E.R., Lawton, A.R., Cooper, M.D.: Pre-B cell leukaemia. New Engl. J. Med. 298, 872–878 (1978)PubMedCrossRefGoogle Scholar
  42. Winchester, R.J., Ross, G.D., Jarowski, C. I., Wang, C.Y., Halper, J., Broxmeyer, H.E.: Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation. Proc. natl. Acad. Sci. USA 74, 4012–4016 (1977)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • M. F. Greaves
    • 1
  1. 1.Membrane Immunology LaboratoryImperial Cancer Research FundLincoln’s Inn Fields, LondonGreat Britain

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