Comparison of Fluorometric Micromethods for Analyzing Erythrocytic Porphyrins (EP)

  • K. H. Schaller
  • R. Schiele


Biologic and biochemical parameters for detection and judgement of increased occupational lead exposure have been known for several decades. This procedure may meet the need to avoid the still relatively sophisticated blood-lead analysis. On the other hand the determination of such data will hopefully lead to additional information about the extense of the harmful effects of lead on organisms (17). Few of the numerous biochemical parameters still used in the field of occupational medicine to determine an increased lead load have proved successful in routine operation. The methods generally used nowadays are of measuring δ-aminolevulinic acid in urine and δ-aminolevulinic-aciddehydratase activity of erythrocytes. Recent examinations confirm that the measurement of erythrocytic porphyrins (EP) is a specific and sensitive criterion of the influence of lead on man (1, 9, 15, 17, 20, 22). Fast and reliable fluorometric micromethods for the EP determination have been developed in recent years (4, 5, 6, 8, 9, 12, 13, 17).


Lead Exposure Lead Poisoning Correlation Diagram Fluorometric Method Zinc Protoporphyrin 
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  1. 1.
    Alessio, L., Bertazzi, P.A., Toffoletto, F., Foa, A.: Free Erythrocyte Protoporphyrin as an Indicator of the Biological Effect of Lead in Adult Males. Int. Arch. Occup. Environ. Health 37, 73–88 (1976)PubMedCrossRefGoogle Scholar
  2. 2.
    Blumberg, W.E., Eisinger, J., Lamola, A.A., Zuckerman, D.M.: The Hematofluorometer. Clin. Chem. 23, 270–274 (1977)PubMedGoogle Scholar
  3. 3.
    Chisholm, J.J., Jr.: Screening techniques for undue lead exposure in children: Biological and practical considerations. J. Pediatr. 79, 719–725 (1971)CrossRefGoogle Scholar
  4. 4.
    Chisholm, J.J., Jr., Mellits, E.D., Keil, J.E., Barret, M.B.: A simple protoporphyrin assay - Microhematocrit procedure as a screening technique for increased lead absorption in young children. Pediatrics 84, 490–496 (1974)CrossRefGoogle Scholar
  5. 5.
    Chisholm, J.J., Brown, D.H.: Micro-Scale photofluorometric determination of “Free Erythrocyte Porphyrin” (Protoporphyrin IX). Clin. Chem. 21, 1669 (1975)Google Scholar
  6. 6.
    Fischbein, A., Eisinger, J., Blumberg, W.E., Lilis, R., Selikoff, I.J.: Fluorometric zinc protoprophyrin determination in blood - a practical method for the detection of chronical lead poisoning. Paper presented at the 2nd international workshop on occupational lead exposure. Amsterdam, 21.-23. Sept. 1976Google Scholar
  7. 7.
    Fischbein, A., Eisinger, J., Blumberg, W.E.: Zinc protoporphyrin determination: A rapid screening test for the detection of lead poisoning. Mt. Sinai J. Med. N.Y. 43, 294–299 (1976)Google Scholar
  8. 8.
    Granick, S., Sassa, S., Granick, J.L., Levere, R.D., Kappas, A.: Assay for porphyrins, δ-aminolevulinic-acid dehydratase, and porphyrinogen synthetase in microliter samples of whole blood: Applications to metabolic defects involving the heme pathway. Proc. Natl. Acad. Sci. U.S.A. 69, 2381–2385 (1972)PubMedCrossRefGoogle Scholar
  9. 9.
    Hanna, Th.L., Dietzler, D.N., Smith, C.H., Gupta, S., Zarkowsky, H.S.: Erythrocyte porphyrin analysis in the detection of lead poisoning in children: Evaluation of four micromethods. Clin. Chem. 22, 161 (1976)PubMedGoogle Scholar
  10. 10.
    Kammholz, L.P., Thatcher, L.G., Blodgett, F.M., Good, Th.A.: Rapid protoporphyrin quantitation for detection of lead poisoning. Pediatrics 50, 625–631 (1972)PubMedGoogle Scholar
  11. 11.
    Lamola, A., Joselow, M., Yamane, T.: Zinc protoporphyrin (ZPP): A simple sensitive fluorometric screening test for lead poisoning. Clin. Chem. 21, 93 (1975)PubMedGoogle Scholar
  12. 12.
    Piomelli, S., Davidow, B., Guinee, V.F., Young, P., Gay, G.: The FEP (free erythrocyte porphyrins) test: A screening micromethod for lead poisoning. Pediatrics 51, 254–259 (1973)PubMedGoogle Scholar
  13. 13.
    Piomelli, S., Young, P., Gay, G.: A micromethod for free erythrocyte porphyrins: The FEP test. J. Lab. Clin. Med. 81, 932–940 (1973)Google Scholar
  14. 14.
    Piomelli, S.: Free Erythrocyte Porphyrins in the Detection of Undue Absorption of Pb and of Fe Deficiency. Clin. Chem. 23, 264–269 (1977)PubMedGoogle Scholar
  15. 15.
    Roels, H.A., Lauwerys, R.R., Buchet, J.P., Vrelust, M.Th.: Response of free erythrocyte porphyrin and urinary 5-aminolevulinic acid in men and women moderately exposed to lead. Int. Arch. Arbeitsmed. 34, 97–108 (1975)PubMedCrossRefGoogle Scholar
  16. 16.
    Sassa, S., Granick, J.L., Granick, S., Kappas, A., Levere, R.D.: Studies in lead poisoning. I. Microanalysis of erythrocyte protoporphyrin levels by spectrofluorometry in the detection of chronic lead intoxiation in the subclinical range. Biochem. Med. 8, 135–148 (1973)PubMedCrossRefGoogle Scholar
  17. 17.
    Schiele, R., Schaller, K.H., Wagner, H.M.: Die Bestimmung der freien Erythrozyten porphyrine als schneller Suchtest einer erhöhten Bleiexposition und seine Validität im Vergleich zum Blutbleispiegel und zur Delta-Aminolaevulinsäure-Dehydratase-Aktivität. Schr.Reihe Ver. Wass.-Boden-Lufthyg. H. 41, 231-240. Stuttgart: Gustav Fischer Verlag 1974Google Scholar
  18. 18.
    Schiele, R., Wagner, H.M., Schaller, K.H.: Die Bestimmung der freien Erythrozyten porphyrine als schneller Suchtest einer beruflichen Bleiexposition. In: Bericht über die 15. Jahrestagung der Dtsch. Ges. f. Arbeitsmed., München 24.-26.4.1975. Brenner, W., Rohmert, W., Rutenfranz, J. (eds.). Stuttgart: A.W. Gentner-Verlag 1976a, pp. 133–139Google Scholar
  19. 19.
    Schiele, R., Schaller, K.H., Valentin, H.: Moderne Kriterien zur Diagnostik und Beurteilung chronischer Bleibelastung in der Arbeitswelt. In: Berufskrankheiten in der keramischen und Glas-Industrie, H. 27, Bad Reichenhaller Kolloquium 1976. Berufsgenossenschaft der keramischen und Glas-Industrie (ed.). Würzburg: 1977, pp. 21-36Google Scholar
  20. 20.
    Schlegel, H.: Laboratoriumsdiagnostik der chronischen Bleivergiftung. Dtsch. Med. Wochenschr. 95, 2131–2132 (1970)PubMedCrossRefGoogle Scholar
  21. 21.
    Schlegel, H., Kufner, G.: Bleivergiftungs-Prävention. Dtsch. Med. Wochenschr. 101, 246–247 (1976)PubMedCrossRefGoogle Scholar
  22. 22.
    Schmidt, D., Stich, W.: Diagnostische Kriterien erhöhter Bleiaufnahme. Münch. Med. Wochenschr. 115, 103–108 (1973)PubMedGoogle Scholar
  23. 23.
    Schwartz, S., Wikoff, H.M.: The relation of erythrocyte coproporphyrin and protoporphyrin to erythropoieses. J. Biol. Chem. 194, 563–573 (1952)PubMedGoogle Scholar
  24. 24.
    Tomokuni, K., Osaka, J., Ogata, M.: Erythrocyte Protoporphyrin Test for Occupational Lead Exposure. Arch. Environ. Health 30, 588–590 (1975)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1978

Authors and Affiliations

  • K. H. Schaller
    • 1
  • R. Schiele
    • 1
  1. 1.Institute for Occupational and Social Medicine and Policlinic for Occupational Diseases of Erlangen-Nürnberg-UniversityGermany

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