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A Gas Chromatographic Method for the Evaluation of the Vinyl Epoxidase Activity

  • E. Malvoisin
  • B. Rollmann
  • M. Roberfroid
  • M. Mercier
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 1)

Abstract

Two alternative specific and very sensitive methods for determination of vinyl epoxide synthetase activity in liver microsomes are described.

Trichloroethylene, which is used as a substrate, is converted into trichloro-ethylene oxide by a hepatic epoxide synthetase. Chloral hydrate, the final rearrangement product of trichloroethylene oxide, is evaluated by electron capture gas chromatography, either after derivatization with pentafluorophenyl-hydrazine or after its conversion into chloroform under alcaline conditions. The kinetic parameters of the epoxidative reaction have been determined on rat hepatic microsomal suspensions.

Keywords

Carbon Tetrachloride Liver Microsome Vinyl Chloride Chloral Hydrate Incubation Mixture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • E. Malvoisin
    • 1
  • B. Rollmann
    • 2
  • M. Roberfroid
    • 1
  • M. Mercier
    • 1
  1. 1.Laboratory of Biotoxicology, School of PharmacyCatholic University of LouvainBruxellesBelgium
  2. 2.Laboratory of Therapeutical Physico-Chemistry, School of PharmacyCatholic University of LouvainBruxellesBelgium

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