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Lysosomal Enzymes in Hepatic Injury Induced by Bromobenzene

  • Pauli V. Luoma
  • Philip S. Guzelian
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 1)

Abstract

In studies with bromobenzene we found increases in the activities of hepatic lysosomal enzymes. The present study was undertaken to determine how these changes are related to the development of hepatic injury after bromobenzene administration. A single intraperitoneal injection of bromobenzene (5 mmoles/kg) caused significant increases in the total activity of lysosomal N-acetyl-β-glucosaminidase and ß-glucuronidase in rat liver within 8–12 h, whereas it did not alter the free activity of these enzymes. Enhancement of bromobenzene hepatotoxicity by the pretreatment of rats with phenobarbital was not associated with further changes in hepatic N-acetyl-ß-glucosaminidase or β-glucuronidase activities. Cycloheximide administered prior to bromobenzene inhibited significantly the effect of bromobenzene on the total N-acetyl-β-glu-cosaminidase activity. The results suggest that lysosomal enzymes are not directly involved in hepatic damage caused by bromobenzene and that the inhibition of protein synthesis may reduce this response to hepatotoxin.

Keywords

Total Activity Lysosomal Enzyme Hepatic Injury Free Activity Hepatic Damage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. DeDuve, C: In: Lysosomes in Biology and Pathology, Vol. I, p. 3. (J.T. Dingle and H.B. Fell, eds.), Amsterdam: North-Holland 1969Google Scholar
  2. Gottwik, M.G., Kirk, E.S., Hoffstein, S., Weglicki, W.B.: J. Clin. Invest. 56, 914–923 (1975)PubMedCrossRefGoogle Scholar
  3. Jollow, DJ., Mitchell, J.R., Jampaglione, N., Gillette, J.R.: Pharmacology 11, 151–169(1974)PubMedCrossRefGoogle Scholar
  4. Niebes, P., Ponard, G.: Biochem. Pharmac. 24, 905–909 (1975)CrossRefGoogle Scholar
  5. Reid, W.D., Krishna, G.: Exp. Mol. Path. 18, 80–99 (1973)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Pauli V. Luoma
    • 1
  • Philip S. Guzelian
    • 1
  1. 1.Department of Medicine, Medical College of VirginiaVirginia Commonwealth UniversityRichmondUSA

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