Immunohistologic Identification of Vertebrate Neurophysins
According to WUU et al. (for review, see WALTER, 1975), there is a striking similarity in the molecular structure of different types of mammalian neurophysins (NP), associated with an extensive immunologic cross-reactivity. The cross-reactivity has been utilized very successfully by many authors in radioimmunoassays and immunohistologic investigations of the mammalian neurosecretory system. Moreover, the staining and histochemical properties of NP of all vertebrates are very similar. Thus, we decided to search for an immunologic relationship between NP within the vertebrate subphylum. The special aim of our work was to find a convenient immunohistologic method for the identification of NP and NP-like substances that would be applicable to all vertebrates; only one type of anti-NP antisera would be used, based on the principle of heterology. WATKINS (1975) started to investigate species-dependent cross-reactivity. In order to study the range limits of this cross-reactivity, we examined immunohistologically the reaction between the antiserum to porcine NP and, on the other hand, NP and NP-like substances inside and outside the hypothalamus in representatives of different vertebrate classes (see also WOLF, 1976).
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