Abstract
Immunoadsorbents have been widely used for purification of specific and highly pure antibodies. Various materials have been employed as carrier-matrices for the binding of specific antigens. The most successful experiments have been made with ion-exchange resins (8), cellulose (3), glutaraldehyde-beads (11) and agarose-beads (5, 6). All methods have in common that the use of gels as carrier-matrices has certain disadvantages as there are: instability to temperature, hydratation conditions, and mechanical difficulties whien used in column chromatography.
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References
Anhut, M.: Über die agglutinierenden, cytotoxischen und präzipitierenden Eigenschaften von Kaninchenantiseren gegen saline Milzextrakte und isolierte Milzzellen der Ratte. Inauguraldissertation Univerität zu Köln 1974
Biozzi, G., Stiffel, C, Mouton, D., Liacopoulos-Briot, M., Decreusefond, C, Bout-hillier, Y.: Etude du Phénomène de l’Immunocytoadhaerence, C, Bouthillier, Y.: Etude du Phénomène de l’Immunocytoadhaerence au Cours de l’Immunisation. Ann.Inst. Pasteur, Suppl. 110, 7 (1966)
Campell, D.H., Luescher, E., Lerman, S.S.: Immunologic Adsorbents, I. Isolation of Antibody by Means of a Cellulose-Protein antigen. Proc.Natl. Acad.Sci. Wash. 37, 575 (1951)
Cuatrecasas, P.: Affinity Chromatography of Macromolecules. Advanc.Enzymol. 36, 29 (1972)
Cuatrecasas, P.: Agarose Derivatives for Purification of Protein by Affinity Chromatography. Nature 228, 1327 (1970)
Cuatrecasas, P.: Insulin-Sepharose: Immunoreactivity and Use in the Purification of Antibody. Biochem.Biophys.Res.Comm. 35, 531 (1969)
Hermann, G.: Über das experimentelle Plasmocytom der Maus, Habilitationsschrift Universität zu Köln (1967)
Isliker, H.C.: Purification of Antibodies by Means of Antigens Linked to Ion Exchange Resins. Ann.N.Y.Acad.Sci. 57, 225 (1953)
Parameritis, J.: Untersuchungen über die Immunantwort und ihre passive biologische Behinderung bei Inkompatibilität im H1-System der Ratte. Inauguraldissertation Univerität zu Köln 1974
Perper, R.T., Zee, T.W., Mickelson, M.M. Purification of Lymphocytes and Platelets by Gradient Centrifugation. J.Lab.Clin.Med. 72, 842 (1968)
Weston, P.D., Avrameas, S.: Proteins Coupled to Polyacrylamide Beads, Using Glutaraldehyde. Biochem.Biophys.Res.Commun. 45, 1574(1972)
Zahn, H., Meienhofer, J.: Reaktionen von l,5-Difluor-2,4-dinitrobenzol mit Insulin, 1. Mitt.: Synthese von Modellverbindungen. Makromol.Chemie 26,126(1958)
Zahn, H., Meienhofer, J.: Reaktionen von l,5-Difluor-2,4-dinitrobenzol mit Insulin, 2.Mitt.: Versuche mit Insulin. Makromol.Chemie 26, 153 (1958)
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© 1977 Springer-Verlag Berlin Heidelberg
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Hermann, G., Trost, T. (1977). Isolation of Antilymphocyte Antibodies, with a Solid Water-Insoluble Not Gel-Like Immunoadsorbent. In: Thierfelder, S., Rodt, H., Thiel, E. (eds) Immunological Diagnosis of Leukemias and Lymphomas. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 20. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66639-1_46
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DOI: https://doi.org/10.1007/978-3-642-66639-1_46
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