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Methods

  • Victoria Chan-Palay
Chapter
  • 106 Downloads

Abstract

The data presented in this book were obtained from the study of normal and experimental material from rats and primates. This investigation involved light microscopy of reference sets of celloidin sections stained by the Nissl and Klüver-Barrera methods; light and electron microscopy of Golgi preparations, electron microscopy of normal and experimental ablation material, fluorescence microscopy, silver and degeneration methods. Some of the experimental approaches used are of recent development: afferent source tracing with horseradish peroxidase (HRP) after retrograde axoplasmic transport; efferent projections tracing by means of anterograde axoplasmic transport of labeled protein; localization of catecholamine and indoleamine axons by light and electron microscopic autoradiography after administration of exogenous radioactive transmitter molecules, their precursors or their analogs. Adaptation and specific technical improvements of these methods were necessary because they were applied to the study of a particular cerebelar nucleus, deeply embedded in the brain, in two different species.

Keywords

Cerebellar Nucleus Cresyl Violet Uranyl Nitrate Bone Flap Amyl Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1977

Authors and Affiliations

  • Victoria Chan-Palay
    • 1
  1. 1.Harvard Medical SchoolBostonUSA

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