Abstract
The thyroid glands were collected aseptically at the slaughter-house from adult Pigs (100–120 kg) and transported rapidly to the laboratory at 4°C. The muscular and fatty tissues surrounding the glands, as well as the capsule and the connective partitions which lobulate them were removed with scissors and forceps. They were then rinsed with physiological saline, minced with scissors, rinsed again and placed in prewarmed trypsin at 35°C (0.25% proteolytic enzymes, pH 7.0 from Institut Pasteur).
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© 1976 Springer-Verlag Berlin-Heidelberg
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Cau, P., Michel-Béchet, M., Fayet, G. (1976). Material and Methods. In: Morphogenesis of Thyroid Follicles in Vitro. Advances in Anatomy, Embryology and Cell Biology/Ergebnisse der Anatomie und Entwicklungsgeschichte /Revues d’anatomie et de morphologie expérimentale, vol 52/2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66334-5_2
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DOI: https://doi.org/10.1007/978-3-642-66334-5_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-07654-4
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