Hemopoietic Dysplasias (Preleukemic States) pp 125-137 | Cite as
In vitro Colony Growth of Acute Myelogenous Leukemia
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Abstract
Colony formation in vitro by marrow cells from patients with untreated acute myelogenous leukemia (AML) and from patients in AML relapse is infrequent using the standard Robinson assay. A newly developed culture system has been described in which marrow from AML patients in these disease stages form leukemic cell colonies. In this in vitro system, phytohaemagglutinin is the essential stimulator for colony formation. The leukemic origin of the colonies has been proven by ultrastructural morphology and cytogenetics. It appears that colony formation by leukemic cells in this system is predominantly independent from the leukocyte factor which is the main stimulator in the Robinson assay for growing colonies of marrow cells from haematologically normal individuals.
Keywords
Acute myelogenous leukemia (AML) Colony formation in AML-PHAPreview
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References
- 1.PIKE, B.L., ROBINSON, W.A.: Human bone marrow colony growth in agar-gel. J. of Cell Phys.76, 77–84, 1970 CrossRefGoogle Scholar
- 2.BULL, J.M., CUTTERA, J.J., NORTHRUP, J.D., HENDERSON, E.S., STASHICK, E., CARBONE, P.P.:Serial in vitro marrow culture in acute myelocytic leukemia. Blood 42, 679–686, 1973 PubMedGoogle Scholar
- 3.GREENBERG, P.L., NICHOLS, W.C., SCHRIER, S.L.: Granulopoiesis in acute myeloid leukemia and pre-leukemia. New Eng. J. of Med. 284, 1225–1232, 1971 CrossRefGoogle Scholar
- 4.MOORE, M.A.S., WILLIAMS, H., METCALF, D.: In vitro colony formation by normal and leukemic human chemotopoietic cells; interraction between colony forming and colony stimulating cells. J. of the Nat. Cancer Inst. 50, 603–623, 1973 Google Scholar
- 5.ROZENSZAJN, L.A., KALECHMAN, I., SHOHUM, D.: Colony proliferation of human stimulated lymphocyte on agar culture; XV, Congress of the International Society of Hem., Page 230, 1974 Google Scholar
- 6.GOLDE, D.W., CLINEK, M.J.: Personal communicationGoogle Scholar
- 7.HAYHOE, G.F.J., QUAGLINO, D., DOLL, R.: The cytology and cytochemistry of acute leukemias. A study of 140 cases. M.R.C. Special Report Series, No. 304, London, Her Majesty’s Stationery Office, 1964 Google Scholar
- 8.MCCREDIE, K.B., HESTER, J.P., GUTTERMAN, J.U., GEHAN, E.A., FREIREICH, E.J.: Survival of adults with acute leukemia CI. Proc. of the AACR 16, 141, 1975 Google Scholar
- 9.GUTTERMAN,J.U., HERSH, E.M., RODRIGUEZ, V., MCCREDIE, K.B., MAVLIGIT, G., REED, R.C., BURGESS, M.A., GEHAN, E.A., BODEY, G.P., FREIREICH, E.J.: Chemoimmunotherapy of acute leukemia: Prolongation of remission in myeloblastic leukemia with bacillus Calmette, Guerin. Lancet, 1405–1409, 1974 Google Scholar
- 10.ROBINSON, W.A., PIKE, B.L.: Colony growth of human bone marrow cells in vitro. Symposium of Haematopoietic Cellular Proliferation St. Elizabeth’s Hospital, Boston. November. Editor: Frederick Stohlman, Jr., Grune & Stratton, New York, 1970 Google Scholar
- 11.DICKE, K.A., TRIDENTE, G., VAN BEKKUM, D.W.: The selective elimination of immunologically competent cells from bone marrow and lymphocyte cell mixtures. III. In vitro test for detection of immunocompetent cells in fractionated mouse spleen cell suspensions and primate bone marrow suspensions. Transplantation 8, 422–434, 1969 PubMedCrossRefGoogle Scholar
- 12.PRIVAL, J.T., PARAN, M., GALLO, R.C., WU, A.M.: Colony-stimulating factors in cultures of human peripheral blood cells. J. of the Nat. Cancer Inst. 53, 1583–1588, 1974 Google Scholar
- 13.DICKE, K.A., VAN NOORD, MAAT, B., SCHAEFER, U.W., VAN BEKKUM, D. W.: Identification of cells in primated bone marrow resembling the haemopoietic stem cell in the mouse. Blood 42, 195–208, 1973 PubMedGoogle Scholar
- 14.ISCOVE, N.N., SIEBER, F.: Erythroid progenitors in mouse bone marrow detected by macroscopic colony formation in culture. Exp. Hemat. 3, 32–43, 1975 PubMedGoogle Scholar
- 15.ACHONG, B.G., EPSTEIN, M.A.: Fine structure of the Burkitt tumor. J. of the Nat. Cancer Inst. 36, 877–897, 1966 Google Scholar
- 16.ANDERSON, D.R.: Ultrastructure of Normal and Leukemic Leukocytes in Human Peripheral Blood. J. of Ultr. Res. 9, 24–27, 1966 Google Scholar
- 17.ROSS, A., HARNDEN, D.: Ultrastructural Studies on Normal and Leukemic Human Haematopoietic Cell. Europ. J. of Cancer 5, 349–360, 1969 CrossRefGoogle Scholar
- 18.AHEARN, M.J., TRUJILLO, J.M., CORK, A., FOWLER, A., HART, J.S.: The association of nuclear blebs with aneuploidy in human acute leukemia. Cancer Res. 34, 2887–2896, 1974 PubMedGoogle Scholar
- 19.TRUJILLO, J.M., CORK, A., HART, J.S., GEORGE, S.L., FREIREICH, E.J.: Clinical implications of aneuploid cytogenetic profiles in adult acute leukemia. Cancer 33, 824–834, 1974 PubMedCrossRefGoogle Scholar
- 20.MOORE, M.A.S., SPITZER, G., WILLIAMS, N., METCALF, D., BUCKLEY, J.: Agar culture studies in 127 cases on untreated acute leukemia; the prognostic value of reclassification of leukemic according to in vitro growth characteristics. Blood 44, 1–8, 1974 PubMedGoogle Scholar
- 21.METCALF, D., MOORE, M.A.S., SHERIDAN, J.W., SPITZER, G.: Responsiveness of human granulo - cytic leukemia cells to colony stimulating factor. Blood 43, 847–859, 1974 PubMedGoogle Scholar
- 22.AYE, M.T., NIHO, Y., TILL, L.E., MCCULLOCH, E.A.: Studies of leukemic cell populations in culture. Blood 4, 205–219, 1974 Google Scholar
- 23.DICKE, K.A., VAN NOORD, M.J., MAAT, B., SCHAEFER, U.W., VAN BEKKUM, D.W.: Attempts at morphological identification of haemopoietic stem cell in primates and rodents. In: Haemopoietic stem cells; CIBA Foundation Symposium No. 13 (New Series). Amsterdam, Elservier. Pages 47–69, 1973 Google Scholar
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