Abstract
Cell culture methods applied to the study of acute leukemia have indicated the presence of many abnormalities. Utilizing this knowledge, we have applied cell culture techniquies to the evaluation of a patient population with a recognized risk of developing leukemia. We demonstrate that cultural abnormalities present in some patients with sideroblastic anemia are similar to those seen in acute leukemia, and that these perturbations may be shown before leukemia develops. This study suggests that cell culture abnormalities may reflect mechanisms operative in vivo, and preceding the development of overt leukemia.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
BLOCK, M., JACOBSON, L.O., BETHARD, W.F.: Preleukemic acute human leukemia. JAMA 152, 1018–1028 1953
CHERVENICK, P.A., LOBUGLIO, A.F.: Human blood monocytes. Stimulators of granulocyte and mononuclear colony formation in vitro. Science 178, 164–166, 1972
CURTIS, J.E., COWAN, D.H., BERGSAGEL, D.E., HASSELBACK, R., MCCULLOCH, E.A.: Adult acute leukemia: Remission induction using combination chemotherapy assessed by clinical and cell culture criteria. CMAJ 113, 289–294, 1975
GREENBERG, P.L., NICHOLS, W.C., SCHRIER, S.L.: Granulopoiesis in acute myeloid leukemia and preleukemia. New Engl. J. Med. 284, 1225–1232, 1971
ISCOVE, N.N., SENN, J.S., TILL, J.E., MCCULLOCH, E.A.: Colony formation by normal and leukemic human marrow cells in culture: Effect of conditioned medium from human leukocytes. Blood 37, 1–5, 1971
MAK, T.W., AYE, M.T., MESSNER, H.A., SHEININ, R., TILL, J.E., MCCULLOCH, E.A.: Reverse transcriptase activity: Increase in marrow cultures from leukemic patients in relapse and remission. Brit. J. Cancer 29, 433–437, 1974
MAK, T.W., MANASTER, J., HOWATSON, A.E., MCCULLOCH, E.A., TILL, J.E.: Particles with characteristic of leukoviruses in cultures of marrow cells from leukemic patients in remission and relapse. Proc. Nat. Acad. Sci. USA 71, 4336–4340, 1974
MAK, T.W., KURTZ, S., MANASTER, J., HOUSMAN, D.: Viral related information in oncornaviruslike particles isolated from cultures of marrow cells from leukemic patients in relapse and remission. Proc. Nat. Acad. Sci. USA 72, 623–627, 1975
MAK, T.W., PRICE, G.B., MILLER, R.G., POTTS, T.V., HOUSMAN, D.E., TILL, J.E., MCCULLOCH, E.A.: Enrichment by velocity sedimentation of human leukemic marrow cells producing viral-relatecl particles in culture. Presented at the Annual Meeting of the American Association for Cancer Research, San Diego, May 6-10, 1975
Moore, C.V.: Sideroblastic Anemia. In: Williams, W.J., Beutler, E., Ersley, A.J., Rundles, R.W., ed. Hematology. New York: McGraw-Hill Inc. 1972, p. 349
MOORE, M.A.S., SPITZER, G., WILLIAMS, N., METCALF, D., BUCKLEY, J.: Agar culture studies in 127 cases of untreated acute leukemia: The prognostic value of reclassification of leukemia according to in vitro growth characteristics. Blood 44, 1–18, 1974
PIKE, B.L., ROBINSON, W.A.: Human bone marrow colony growth in agar-gel. J. Cell. Physiol. 76, 77–84, 1970
PRICE, G.B., MCCULLOCH, E.A., TILL, J.E.: A new human low molecular weight granulocyte colony stimulating activity. Blood 42, 341–348, 1973
PRICE, G.B., SENN, J.S., MCCULLOCH, E.A., TILL, J.E.: The isolation and properties of granulocytic colony stimulating activities from human peripheral leukocyte conditioned medium. Biochemical J. 148, 209–317, 1975
PRICE, G.B., MCCULLOCH, E.A., TILL, J.E.: Cell membranes as sources of granulocyte colony stimulating activity. Expt. Haemat. (in press) 1975
SENN, J.S., MCCULLOCH, E.A., TILL, J.E.: Comparison of the colony forming ability of normal and leukemic human marrow in cell culture. Lancet ii, 597–598, 1967
SENN, J.S., PINKERTON, P.H.: Defective in vitro colony formation by human bone marrow preceding over leukemia. Brit. J. Haemat. 23, 277–281, 1972
SENN, J.S., MESSNER, H.A., STANLEY. E.R.: Analysis of interacting cell populations in cultures of marrow from patients with neutropenia. Blood 44, 33– 40, 1974
SENN, J.S., PRICE, G.B., PINKERTON, P.H.: Granulopoiesis in culture: Studies of patients with sideroblastic anemia. Presented at the Annual Meeting, American Society of Hematology, Atlanta. December 7–10, 1974
SENN, J.S., PINKERTON, P.H.: Sideroblastic anemia considered as preleukemia. Presented at Annual Meeting, American Association for Cancer Research, San Diego, May 6-10, 1975
VOSIKA, G.J., KRIVIT, W., GERRARD, J.M., COCCIA, P.F., NESBIT, M.E., COALSON, J.J., KENNEDY, B.J.: Oncorna-virus-like particles from cultured bone marrow cells preceding leukemia and malignant histiocytosis. Proc. Nat. Acad. Sci. USA 72, 2804–2808, 1975
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1977 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Senn, J.S., Price, G.B., Mak, T.W., McCulloch, E.A. (1977). An Approach to Human Preleukemia Using Cell Culture Studies. In: Bessis, M., Brecher, G. (eds) Hemopoietic Dysplasias (Preleukemic States). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66312-3_11
Download citation
DOI: https://doi.org/10.1007/978-3-642-66312-3_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-07597-4
Online ISBN: 978-3-642-66312-3
eBook Packages: Springer Book Archive