Advertisement

Cell Division and DNA Replication in Synchronous Tetrahymena Cultures

  • H. A. Andersen
  • Leif Rasmussen
  • Erik Zeuthen
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 72)

Abstract

Several methods are available for the induction of synchronous cell divisions in pro- and eukaryote cells. The present article deals almost exclusively with the synchronization of the ciliate protozoan Tetrahymena pyriformis, which is one of the cells most intensively studied in synchronous populations. As it will appear from the following pages Tetrahymena has been synchronized with several different procedures. The first of these was the multiple heat shock system which produced well-synchronized cell divisions. Later modification of the original programme synchronized not only cell division but also DNA replication. Since most of the work based on synchronized Tetrahymena cells has been done with heat-synchronized cells, recent results obtained with such cells will take up most of this review.

Keywords

Heat Shock Cell Division Cell Generation Temperature Shock Cell BioI 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ammermann, D.: Morphology and development of the macronuclei of the ciliates Stylonychia mytilus and Euplotes aediculatus. Chromosoma 33, 209–238 (1971)PubMedCrossRefGoogle Scholar
  2. Andersen, H. A.: Requirements for DNA replication preceding cell division in Tetrahymena pyriformis. Exptl. Cell Res. 75, 89–94 (1972)PubMedCrossRefGoogle Scholar
  3. Andersen, H. A., Brunk, C. F., Zeuthen, E.: Studies on the DNA replication in heat synchronized Tetrahymena pyriformis. Compt. Rend. Trav. Lab. Carlsberg 38, 123–131 (1970)Google Scholar
  4. Andersen, H.A., Engberg, J.: Timing of the ribosomal RNA gene replication in Tetrahymena pyriformis. Exptl. Cell Res. 92, 159–163 (1975)PubMedCrossRefGoogle Scholar
  5. Andersen, H. A., Zeuthen, E.: DNA replication sequence in Tetrahymena is not repeated from generation to generation. Exptl. Cell Res. 68, 309–314 (1971)PubMedCrossRefGoogle Scholar
  6. Buhse, H. E. Jr., Rasmussen, L.: A method for synchronization of cell division and macrostome formation in the ciliate Tetrahymena vorax. Compt. Rend. Trav. Lab. Carlsberg 40, 59–67 (1974)Google Scholar
  7. Cameron, I. L., Bols, N. C.: Effect of cell population density on G2 arrest in Tetrahymena. J. Cell Biol. 63, 46a (1974)Google Scholar
  8. Cameron, I. L., Jeter, J. R.: Synchronization of the cell cycle of Tetrahymena by starving—refeeding. Protozool. 17, 429–433 (1970)Google Scholar
  9. Cameron, I. L., Nachtwey, D. S.: DNA synthesis in relation to cell division in Tetrahymena pyriformis. Exptl. Cell Res. 46, 385–395 (1967)PubMedCrossRefGoogle Scholar
  10. Cameron, I. L., Padilla, G. M., Miller, O. L.: Macronuclear cytology of synchronized Tetrahymena pyriformis. J. Protozool. 13, 336–341 (1966)PubMedGoogle Scholar
  11. Chapman-Andresen, C., Nilsson, J. R.: On vacuole formation in Tetrahymena pyriformis. GL. Comp. Rend. Trav. Lab. Carlsberg 36, 405–432 (1968)Google Scholar
  12. Charret, R.: L’ADN nucléolaire chez Tetrahymena pyriformis: Chronologie de sa réplication. Exptl. Cell Res. 54, 353–361 (1969)PubMedCrossRefGoogle Scholar
  13. Cleffmann, G.: Regulierung der DNS-Menge im Makronukleus von Tetrahymena. Exptl. Cell Res. 50, 193–207 (1968)PubMedCrossRefGoogle Scholar
  14. Cohr, K. H.: Dyrkning og synkronisering af Tetrahymena i kontinuerte kulturer. Thesis, University of Copenhagen 1973Google Scholar
  15. Debault, L. E., Ringertz, N. R.: A comparison of normal and cold synchronized cell divisions in Tetrahymena. Exptl. Cell Res. 45, 509–518 (1967)PubMedCrossRefGoogle Scholar
  16. Edmunds, L. N. Jr.: Phasing effects of light on cell division in exponentially increasing cultures of Tetrahymena grown at low temperatures. Exptl. Cell Res. 83, 367–379 (1974)PubMedCrossRefGoogle Scholar
  17. Engberg, J., Mowat, D., Pearlman, R. E.: Preferential replication of the ribosomal DNA genes during a nutritional shiftup in Tetrahymena pyriformis. Biochem. Biophys. Acta 272, 312–320 (1972)Google Scholar
  18. Engberg, J., Nilsson, J. R., Pearlman, R. E., Leick, V.: Induction of nucleolar and mitochondrial DNA replication in Tetrahymena pyriformis. Proc. Natl. Acad. Sci. U.S. 71, 894–898 (1974a)CrossRefGoogle Scholar
  19. Engberg, J., Christiansen, G., Leick, V.: Autonomous rDNA molecules containing single cepies of the ribosomal RNA genes in the macronucleus of Tetrahymena pyriformis. Biochem. Biophys. Res. Comm. 59, 1356–1365 (1974b)PubMedCrossRefGoogle Scholar
  20. Frankel, J.: Morphogenesis and division in chains of Tetrahymena pyriformis GL. J. Protozool. 11, 514–526 (1964)PubMedGoogle Scholar
  21. Gall, J. G.: Free ribosomal RNA genes in the macronucleus of Tetrahymena. Proc. Natl. Acad. Sci. 71, 3078–3081 (1974)PubMedCrossRefGoogle Scholar
  22. Hildebrandt, A., Duspiva, F.: Eine einfache Methode zur Synchronization größerer Populationen des Ciliaten Tetrahymena pyriformis (Ehrenberg). Z. Naturforsch. 24b, 747–750 (1969)Google Scholar
  23. Hjelm, K. K., Zeuthen, E.: Synchronous DNA synthesis induced by synchronous cell division in Tetrahymena. Compt. Rend. Trav. Lab. Carlsberg 36, 127–160 (1967)Google Scholar
  24. Hjelm, K. K.: A technique for cultivation of Tetrahymena in rotating bottles. Exptl. Cell Res. 60, 191–198 (1970)PubMedCrossRefGoogle Scholar
  25. Hjelm, K. K.: A synchronizing effect of carbon dioxide on cell division in Tetrahymena. Exptl. Cell Res. 67, 204–207 (1971)PubMedCrossRefGoogle Scholar
  26. Holm, B. F.: Changes in the amount of DNA in synchronized cultures of Tetrahymena cells. Exptl. Cell Res. 53, 18–36 (1968)PubMedCrossRefGoogle Scholar
  27. Holz, G. G., Scherbaum, O. H., Williams, N.: The arrest of mitosis and stomato-genesis during temperature-induction of synchronous division in Tetrahymena pyriformis, mating type 1, variety 1. Exptl. Cell Res. 13, 618–621 (1957)PubMedCrossRefGoogle Scholar
  28. Jeffery, W. R.: Evidence for a temporal incompatibility between DNA replication and division during the cell cycle of Tetrahymena. J. Cell Biol. 53, 624–634 (1972)PubMedCrossRefGoogle Scholar
  29. Jeffery, W. R., Frankel, J., Debault, L. E., Jenkins, L. M.: Analysis of the schedule of DNA replication in heat-synchronized Tetrahymena. J. Cell Biol. 59, 1–11 (1973)PubMedCrossRefGoogle Scholar
  30. Jeffery, W. R., Stuart, K. D., Frankel, J.: The relationship between deoxyribonucleic acid replication and cell division in heat-synchronized Tetrahymena. J. Cell Biol. 46, 533–543 (1970)PubMedCrossRefGoogle Scholar
  31. Kimball, R. F., Prescott, D. M.: Deoxyribonucleic acid synthesis and distribution during growth and amitosis of the macronucleus of Euplotes. J. Protozool. 9, 88–92 (1962)Google Scholar
  32. Koyama, H., Ono, T.: Effect of 5-bromodeoxyuridine on hyaluronic acid synthesis of a mouse and Chinese hamster in culture. J. Cell Physiol. 78, 265–272 (1971)PubMedCrossRefGoogle Scholar
  33. Kramhøft, B., Zeuthen, E.: Synchronization of cell divisions in the fission yeast, Schizosaccharomyces pombe, using heat shocks. Compt. Rend. Trav. Lab. Carlsberg 38, 351–368 (1971) (ed. D. M. Prescott.)Google Scholar
  34. KramhØft, B., Zeuthen, E.: Synchronization of the fission yeast, Schizosaccharomyces pombe, using heat shocks. In: Methods in Cell Biology XII. New York: Academic Press 1975Google Scholar
  35. Kuzmich, M. J., Zimmerman, A. M.: Colcemid action on the division schedule of synchronized Tetrahymena. Exptl. Cell Res. 72, 441–452 (1972)PubMedCrossRefGoogle Scholar
  36. Lykkesfeldt, A. E., Andersen, H. A.: The effect of 5-bromodeoxyuridine on DNA replication and cell division in Tetrahymena pyriformis. J. Cell Biol. 62, 316–321 (1974)PubMedCrossRefGoogle Scholar
  37. Lykkesfeldt, A. E., Andersen, H. A.: Inhibition of rRNA synthesis following incorporation of 5-bromodeoxyuridine into DNA of Tetrahymena pyriformis. J. Cell Sci. 17, 495–502 (1975)PubMedGoogle Scholar
  38. Mitchison, J. M.: The Biology of the Cell Cycle. Cambridge: Univ. Press 1971Google Scholar
  39. Miyamoto, H., Rasmussen, L., Zeuthen, E.: Studies of the effect of temperature shocks on preparation for cell division in mouse fibroblast cells (L cells). J. Cell Sci. 13, 889–900 (1973)PubMedGoogle Scholar
  40. Miyamoto, H., Rasmussen, L., Zeuthen, E.: Recording of clonal growth of mammalian cells through many generations. In: Methods in Cell Biology XIII. New York: Academic Press 1975 (ed. D. M. Prescott).Google Scholar
  41. Moner, J. G., Berger, R. O.: RNA synthesis and cell division in cold-synchronized cells of Tetrahymena pyriformis. J. Cell Physiol. 67, 217–223 (1966)PubMedCrossRefGoogle Scholar
  42. Mowat, D., Pearlman, R. E., Engberg, J.: DNA synthesis following refeeding of starved Tetrahymena pyriformis GL. Exptl. Cell Res. 84, 282–286 (1974)PubMedCrossRefGoogle Scholar
  43. Murakami, T. M., Zimmerman, A. M.: DNA synthesis in Tetrahymena a pressure study. Cytobios 7, 171–181 (1973)Google Scholar
  44. Nilsson, J. R.: Suggestive structural evidence for macronuclear “subnuclei” in Tetrahymena pyriformis, GL. J. Protozool. 17, 539–548 (1970)PubMedGoogle Scholar
  45. Prescott, D. M.: Variations in the individual generation times of Tetrahymena geleii HS. Exptl. Cell Res. 16, 279–284 (1959)PubMedCrossRefGoogle Scholar
  46. Rasmussen, L., Cohr, K. H., Buhse, H. E. Jr., Zeuthen, E.: Automatic cell counting in continuous flow cultures of Tetrahymena pyriformis. J. Protozool. 21, 552–555 (1974)PubMedGoogle Scholar
  47. Rudick, M. J., Cameron, I. I.: Regulation of DNA synthesis and cell division in starved-refed synchronized Tetrahymena pyriformis HSM. Exptl. Cell Res. 70, 411–416 (1972)PubMedCrossRefGoogle Scholar
  48. Scherbaum, O.: The content and composition of nucleic acids in normal and synchronously dividing mass cultures of Tetrahymena pyriformis. Exptl. Cell Res. 13, 24–30 (1957)PubMedCrossRefGoogle Scholar
  49. Sedgley, N. N., Stone, G. E.: DNA synthesis in vinblastine synchronized Tetrahymena. Exptl. Cell Res. 56, 174–177 (1969)PubMedCrossRefGoogle Scholar
  50. Sonneborn, T. H.: Genetics of Tetrahymena pyriformis. Handbook of Genetics. Vol. II (ed. R. C. King). New York: Plenum Press 1974Google Scholar
  51. Stellwagen, R. H., Tomkins, G. M.: Preferential inhibition by 5-bromodeoxyuridine of the synthesis of tyrosine aminotransferase in hepatoma cell cultures. J. Mol. Biol. 56, 167–182 (1971)PubMedCrossRefGoogle Scholar
  52. Stone, G. E.: Synchronized cell division in Tetrahymena pyriformis following inhibition with vinblastine. J. Cell Biol. 39, 556–563 (1968)PubMedCrossRefGoogle Scholar
  53. Villadsen, I. S., Zeuthen, E.: Synchronization in Tetrahymena populations by temporary limitation of access to thymine compounds. Exptl. Cell Res. 61, 302–310 (1970)PubMedCrossRefGoogle Scholar
  54. Williams, N. E.: Induced division synchrony in Tetrahymena vorax. J. Protozool. 11, 230–236 (1964)PubMedGoogle Scholar
  55. Wolfe, J.: Differential density labeling and gradient centrifugation of Tetrahymena. Exptl. Cell Res. 77, 232–238 (1973)PubMedCrossRefGoogle Scholar
  56. Woodward, J., Kaneshiro, E., Gorovsky, M. A.: Cytochemical studies on the problem of macronuclear subnuclei in Tetrahymena. Genetics 70, 251–260 (1972)Google Scholar
  57. Wunderlich, F., Peyk, D.: Antimitotic agents and macronuclear division of ciliates. Exptl. Cell Res. 57, 142–144 (1969)PubMedCrossRefGoogle Scholar
  58. Zeuthen, E.: The temperature-induced division synchrony in Tetrahymena. In: Synchrony in Cell Division and Growth (ed. E. Zeuthen). New York: Interscience Publishers 1964Google Scholar
  59. Zeuthen, E.: Thymine starvation by inhibition of uptake and synthesis of thymine-compounds in Tetrahymena. Exptl. Cell Res. 50, 37–46 (1968)PubMedCrossRefGoogle Scholar
  60. Zeuthen, E.: Synchrony in Tetrahymena by heat shocks spaced a normal cell generation apart. Exptl. Cell Res. 68, 49–60 (1971a)PubMedCrossRefGoogle Scholar
  61. Zeuthen, E.: Synchronization of the Tetrahymena cell cycle. In: Advances in Cell Biology. Vol. II (eds. D.M. Prescott, L. Goldstein, E. McConkey). New York: Appleton, Century and Crofts 1971 bGoogle Scholar
  62. Zeuthen, E.: A cellular model for repetitive and free-running synchrony in Tetrahymena and Schizosaccharomyces. In: Cell Cycle Controls (eds. G.M. Padilla, J.L. Cameron, A. Zimmerman). New York: Academic Press 1974Google Scholar
  63. Zeuthen, E., Rasmussen, L.: Synchronized cell division in protozoology. In: Research in Protozoology. Vol. IV (ed. T. T. Chen). Oxford: Pergamon Press 1972Google Scholar
  64. Zeuthen, E., Scherbaum, O.: Synchronous divisions in mass cultures of the ciliate Protozoon Tetrahymena pyriformis, as induced by temperature changes. In: Recent Development in Cell Physiology (ed. J. A. Kitching). London: Butterworths 1954Google Scholar
  65. Zimmerman, A. M., Laurence, H. L.: Induction of division synchrony in Tetrahymena pyriformis: A pressure study. Exptl. Cell Res. 90, 119–126 (1975)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1975

Authors and Affiliations

  • H. A. Andersen
    • 1
  • Leif Rasmussen
    • 1
  • Erik Zeuthen
    • 1
  1. 1.The Biological Institute of the Carlsberg FoundationCopenhagen NDenmark

Personalised recommendations