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The Use of B and T Membrane Markers in the Classification of Human Leukemias, with Special Reference to Acute Lymphoblastic Leukemia

  • Jean-Claude Brouet
  • Jean-Louis Preud’Homme
  • Maxime Seligmann
Conference paper

Abstract

Cells from patients with leukemias were studied using surface immunoglobulins and IgG aggregates as B cell markers, spontaneous rosette formation with sheep red cells and cytotoxic tests using heterologous antisera for identifying T cells. The vast majority of chronic lymphocytic Leukemias (CLL) was shown to be a monoclonal B cell proliferation with a maturation block. However biclonal proliferations were encountered and a T cell origin was proven in 5 out of 170 CLL. The abnormal cells in 14 patients with the Sezary syndrome were identified as T cells. In most patients with common acute lymphoblastic leukemia, no B or T markers were detected at the surface of the abnormal cells. These cells possessed neo antigens reactive with non anti-B antibodies present in antisera to CLL cells. In 30% of the cases the T nature of the blast cells appeared likely. In 11 other ALL patients a monoclonal B cell proliferation was found. These patients were usually not affected with common ALL and belonged mostly to two specific entities; in 3 cases the blastic proliferation supervent in patients previously affected with common CLL and in 6 cases the blast cells possessed all the cytological features of Burkitt’s tumor cells.

Key Words

Membrane immunoglobulins Lymphoblastic leukemia 

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Copyright information

© Springer-Verlag Berlin Heildelberg 1975

Authors and Affiliations

  • Jean-Claude Brouet
  • Jean-Louis Preud’Homme
  • Maxime Seligmann
    • 1
  1. 1.Laboratory of Immunochemistry, (INSERM U 108), Research Institute on Blood DiseasesHôpital Saint-LouisParisFrance

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