Control of the Epstein-Barr Virus Genome by Mammalian Cells
The Epstein-Barr virus (EBV) originally described in 1964 by Epstein and Barr (1) and EBV-specific antigens have been observed in lymphoblastoid cell lines from Burkitt tumors. Established lymphoblastoid cell lines examined to date are of two types: those that contain EBV markers, such as the early antigens (EA), virus capsid antigen (VCA), membrane antigen (MA), and virus particles, such as P3J-HR-1 (HR-1), and cells that do not produce antigens or virus particles, such as Raji cells. All cell lines, however, whether positive or negative for EB virus markers, contain EBV-specific nucleic acid associated with the genome of the cell (4,16,18). The EBV genome, however, is maintained in a repressed state, i.e., total repression in EB negative cells and partial repression in EB producer cells. What controls the repressed state and regulates the expression of the EB virus genome is central to an understanding of the relationship of the transforming virus and the transformed cell in which the virus genome resides.
KeywordsHybrid Cell Lymphoblastoid Cell Line Raji Cell Somatic Cell Hybrid Cloning Efficiency
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