Evidence for RNA Virus Associated with Human Transitional Cell Cancers of the Urinary Tract
Evidence that there is a ribonucleic acid (RNA) virus associated with transitional cell cancers (TCC) of the human renal pelvis, ureter, and bladder has been reported (2,4,9,10). This virus was partially characterized after passage through chicken embryo fibroblasts (CEF). These studies showed that the virus contained RNA and that it had a morphology similar to type-C animal RNA tumor viruses; a buoyant density of 1.14 to 1.17 g/ml; and RNA-directed DNA polymerase enzyme, as demonstrated by exogenous and endogenous assay techniques (4). This agent produced a cytopathology in CEF and human kidney cells. Also, cells manifesting cytopathology contained virus particles resembling those seen in the original tumors and in cultured tumor cells. In addition, the sera of patients with TCC neutralized the ability of the agent coming directly from primary TCC tissue cultures to produce cytopathology in CEF assay cells (4).
KeywordsSucrose Glycerol Agar Manganese Adenocarcinoma
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