Evidence for RNA Virus Associated with Human Transitional Cell Cancers of the Urinary Tract
Evidence that there is a ribonucleic acid (RNA) virus associated with transitional cell cancers (TCC) of the human renal pelvis, ureter, and bladder has been reported (2,4,9,10). This virus was partially characterized after passage through chicken embryo fibroblasts (CEF). These studies showed that the virus contained RNA and that it had a morphology similar to type-C animal RNA tumor viruses; a buoyant density of 1.14 to 1.17 g/ml; and RNA-directed DNA polymerase enzyme, as demonstrated by exogenous and endogenous assay techniques (4). This agent produced a cytopathology in CEF and human kidney cells. Also, cells manifesting cytopathology contained virus particles resembling those seen in the original tumors and in cultured tumor cells. In addition, the sera of patients with TCC neutralized the ability of the agent coming directly from primary TCC tissue cultures to produce cytopathology in CEF assay cells (4).
KeywordsBuoyant Density Gradient Fraction Chicken Embryo Fibroblast Cheek Pouch Urothelial Tumor
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- 1.Castro, A. E., Bronson, D. L., Elliott, A. Y., Cleveland, P., and Fraley, E. E. Detection of RNA-directed DNA polymerase and high molecular weight RNA in particles isolated from human transitional cell cancers of the urinary tract. Nature. (Manuscript submitted.)Google Scholar
- 6.Elliott, A. Y., Fraley, E. E., Cervenka, J., Cleveland, P., and Stein, N. In vitro neoplastic transformation of mammalian cells produced by acellular fluids of cultured human transitional cell cancers of the urinary tract. (Manuscript in preparation.)Google Scholar
- 9.Fraley, E. E., Elliott, A. Y., and Hakala, T. R. RNA virus isolation from papillary tumors of the renal pelvis. Surg. Forum, XIII:529, 1972.Google Scholar
- 18.Priori, E. S., Wilbur, J. R., and Dmochowski, L. Immunofluorescence tests on sera of patients with osteogenic sarcoma. J. Natl. Cancer Inst., 46:129, 1971.Google Scholar