An Experimental Model for Exocytosis of Chromaffin Granules
One of the more intriguing aspects of the biochemistry and biophysics of synaptic transmission which is being investigated in V. P. Whittaker’s laboratory concerns the mechanism whereby the neurotransmitter, which is stored in the synaptic vesicle storage granules, is released into the synaptic cleft — the space between the pre-and post-synaptic cells. The model for which the most evidence has been accumulated is exocytosis [1–3]. In this model (Fig. 1), the membrane of the storage granule fuses with the pre-synaptic membrane. A connection between the interior of the storage granule and the exterior of the cell is established, and the granule contents are emptied into the synaptic cleft. I would like to comment upon an in vitro system which may help to elucidate the processes involved in the membrane fusion which is required by the model [4, 5].
KeywordsEDTA Sedimentation Catecholamine Ghost
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