Parthenogenetic Activation and Development

  • M. Ishikawa


In 1887, O. and R. HERTWIG reported that fertilization membrane formation in Paraoentrotus lividus could be artificially induced by shaking with chloroform, and R. HERTWIG (1895, 1896) found that artificial cleavage resulted from treating the eggs with strychnine. By exposing the eggs to sea water containing NaCl, KCl, or MgCl2, MORGAN (1899) obtained embryos that cleaved to about 64 cells. In 1899, LOEB obtained partheno-genetically developed Avbacia punotulata plutei using MgCl2-sea water and, in 1904 improved the method of artificial parthenogenesis by combining the membrane-forming and hypertonic treatments. PASPALEFF (192 5) published a study on the cytology of activated eggs.


Butyric Acid Membrane Formation Hypertonic Solution Potassium Cyanide Sulfhydryl Reagent 
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  1. BATAILLON, E., 1929. Analyse de la fécondation par la parthénogénèse expérimentale. Arch. f. Entw. 115, 707–778.CrossRefGoogle Scholar
  2. BRACHET, J., FICQ, A., Tencer, R., 1963. Amino acid incorporation into proteins of nucleate and anucleate Fragments of sea urchin eggs: Effect of parthenogenetic activation. Exptl. Cell Res. 32, 168–170.PubMedCrossRefGoogle Scholar
  3. DELAGE, Y., 1909. Le sexe chez les oursins issus de parthénegénèse expérimentale. C.R. Acad. Sci. Paris 148, 453–455.Google Scholar
  4. DENNY, P.C., TYLER, A., 1964. Activation of protein biosynthesis in non-nucleate fragments of sea urchin eggs. Biochem. Biophys. Res. Comm. 14, 245–249.PubMedCrossRefGoogle Scholar
  5. DIRKSEN, E.R., 1966. The presence of centrioles in artificially activated sea urchin eggs. Jour. Biophys. Biochem. Cytol. 11, 244–247.CrossRefGoogle Scholar
  6. HARVEY, E.B., 1936. Parthenogenetic merogony or cleavage without nuclei in Arbacia punctulata. Biol. Bull. 71, 101–121.CrossRefGoogle Scholar
  7. HARVEY, E.B., 1940. A comparison of the development of nucleate and non-nucleate eggs of Arbacia punctulata. Biol. Bull. 79, 166–187.CrossRefGoogle Scholar
  8. HARVEY, E.B., 1956. The American Arbacia and other Sea Urchins. Princeton: Princeton Univ. Press.Google Scholar
  9. HERBST, C., 1893. Über die künstliche Hervorrufung von Dottermembra-nen an unbefruchteten Seeigeleiern nebst einigen Bemerkungen über die Dotterhautbildung überhaupt. Biol. Zentr. 13, 14.Google Scholar
  10. HERTWIG, O., HERTWIG, R., 1887. Über den Befruchtungs- und Teilungsvorgang des tierischen Eies unter dem Einfluß äußerer Agentien. Jena. Zeitschr. 20, 120–242.Google Scholar
  11. HERTWIG, R., 1895. Über Centrosoma und Centralspindel. Sitzungsb. Ges. Morph, und Physiol., München 11, 41–59.Google Scholar
  12. HERTWIG, R., 1896. Über die Entwicklung des unbefruchteten Seeigeleies. Festschr. f. Gegenbaur, Leipzig 2, 21–86.Google Scholar
  13. ISHIKAWA, M., 1954a. The activation of sea urchin eggs by means of phenols. Embryologia 2, 51–56.CrossRefGoogle Scholar
  14. ISHIKAWA, M., 1954b. Effects of hypertonic sea water on the sea urchin egg in the process of artificial parthenogenesis (in Japanese). Zool. Mag. (Tokyo) 63, 161.Google Scholar
  15. ISHIKAWA, M., 1957. Antagonistic effects of some chemicals on the induction of parthenogenetic development of the sea urchin egg by hypertonic treatment. Embryologia 3, 261–266.CrossRefGoogle Scholar
  16. ISHIKAWA, M., 1960. The effects of sulfhydryl reagents on artificial parthenogenesis in the sea urchin egg. Embryologia 5, 85–94.CrossRefGoogle Scholar
  17. ISHIKAWA, M., 1962. The relation between the incorporation of phosphorus and the induction of cleavage by hypertonic treatment in the sea urchin egg. Embryologia 7, 109–126.CrossRefGoogle Scholar
  18. JUST, E.E., 1922. Initiation of development in the egg of Arbacia. I-III. Biol. Bull. 43, 384–422.CrossRefGoogle Scholar
  19. LITCHFIELD, J.B., WHITELEY, A.H., 1959. Studies on the mechanism of phosphate accumulation by sea urchin embryos. Biol. Bull. 117, 133–149.CrossRefGoogle Scholar
  20. LOEB, J., 1899. On the nature of the process of fertilization and the artificial production of normal larvae (Plutei) from the unfertilized eggs of the sea urchin. Am. Jour. Physiol. 3, 135–138.Google Scholar
  21. LOEB, J., 1904. Über Befruchtung, künstliche Parthenogenese und Cytolyse des Seeigeleies. Pflüger’s Arch. f. ges. Physiol. 103, 257–265.CrossRefGoogle Scholar
  22. LOEB, J., 1904. Über Befruchtung, künstliche Parthenogenese und Cytolyse des Seeigeleies. Trans, in Univ. Cal. Pub. 2, 73–81 (1905).Google Scholar
  23. LOEB, J., 1906. Versuche über den chemischen Charakter des Befruchtung svorgangs. Biochem. Zeitschr. 1, 183–206.Google Scholar
  24. LOEB, J., 1913. Artificial Parthenogenesis and Fertilization. Univ. Chicago Press, Chicago.Google Scholar
  25. MORGAN, T.H., 1899. The action of salt solutions on the unfertilized and fertilized eggs of Arbacia and of ether animals. Arch, f. Entw. 8, 448–539.CrossRefGoogle Scholar
  26. NAKANO, E., GIUDICE, G., MONROY, A., 1958. On the incorporation of S35-methionine in artificially activated sea urchin eggs. Experientia 14, 11–13.CrossRefGoogle Scholar
  27. NAKASHIMA, S., SUGIYAMA, M., 1969. Incorporation of amino acids into sea urchin eggs stimulated insufficiently with an activating reagent. Develop. Growth Differ. 11, 115–122.CrossRefGoogle Scholar
  28. PASPALEFF, G., 1925. Cytologische Untersuchungen an stimulierten Seeigeleiern. Zellstimulationsforsch. 1, 413–451.Google Scholar
  29. RUNNSTRöM, J., HAGSTRöM, B.E., PERLMANN, P., 1959. Fertilization, in The Cell-Biochemistry, Physiology, Morphology. Edited by J. Brächet and A.E. Mirsky, Vol. 1, New York: Academic Press Inc.Google Scholar
  30. SHEARER, C., LLOYD, D.J., 1913. On methods of producing artificial parthenogenesis in Echinus esculentus and the rearing of the partheno-genetic plutei through metamorphosis. Quart. Jour. Microscop. Sci. 58, 523–551.Google Scholar
  31. UTO, N., SUGIYAMA, M., 1969. Incorporation of thymidine-3H into the activated sea urchin egg with special reference to subthreshold stimulation with an activating reagent. Develop. Growth Differ. 11, 123–129.CrossRefGoogle Scholar
  32. Von LEDEBUR-VILLIGER, M., 1972. Cytology and nucleic acid synthesis of parthenogenetically activated sea urchin eggs. Exp. Cell Res. 72, 285–308.CrossRefGoogle Scholar
  33. WILSON, E.B., 1925. The Cell in Development and Heredity. 3rd edition, New York: Macmillan Co.Google Scholar
  34. YAMAMOTO, T., 1956. Chloretone as a parthenogenetic agent in sea-urchin eggs. Embryologia 3, 81–87.CrossRefGoogle Scholar

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© Springer-Verlag Berlin · Heidelberg 1975

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  • M. Ishikawa

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