Ēthrane pp 212-213 | Cite as

Effect of Ēthrane, Halothane and Penthrane on Alveolar Surfactants

  • A. Gasparetto
  • G. Barusco
Conference paper
Part of the Anaesthesiology and Resuscitation / Anaesthesiologie und Wiederbelebung / Anesthésiologie et Réanimation book series (A+I, volume 84)


The phenomenon of changed pulmonary compliance, both during and after general anesthesia, is well known. It is not easily interpreted and is probably due to a number of factors. In a study designed to check the modification of the alveolar surfactant induced by some halogenated agents liposoluble with varying degrees of difficulty, 130 male Sprague-Dawley rats (body weight roughly 220 – 230 g) were anesthetized with Halothane, Penthrane or Ēthrane. Some of the animals were pretreated for ten days with CDPcholine (7 mg/kg i. p.). We examined the isolated lungs and considered
  1. a)

    the respiratory mechanism with reference to the curve for tension versus surface area. The stage of this curve at which the lungs become inflated depends on several factors, i. e. surface tension, initial pressure, and elasticity of the lungs. The curve obtained during deflation, in contrast, is an obvious indication of the strength due to surface tension. A statistical comparison of the values obtained from the deflation curve for transpulmonary pressure was made: these values reflected modifications in the strength of the alveolar surface.

  2. b)

    The chemical composition of the alveolar surfactant was determined by washing the lung wi th a physiological solution that was cooled to 4°C and extracted by Folch’s method, before being analyzed by thin-layer chromatography.

  3. c)

    The histological picture showed prominent histochemical pneumocytes Type II, and the cells that produce the alveolar surfactant were visualized by fixing and staining by the mixed Os-I-Method.



Surface Tension Phosphatidyl Choline Volatile Anesthetic Respiratory Mechanism Histological Picture 
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Copyright information

© Springer Verlag Berlin Heidelberg 1974

Authors and Affiliations

  • A. Gasparetto
  • G. Barusco

There are no affiliations available

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