Abstract
In the yolk-sac syncytium of the muskellunge embryo, Esox masquinongy ohioensis Kirtland, there are nuclei whose size and staining properties with basic dyes suggest that their chromatin content is greater than that of other embryonic nuclei (Bachop, 1965). This study is intended to test that supposition, using a histochemical test specific for one of the constituents of chromatin and using a microspectrophotometer to quantify the histochemical reaction in different nuclei relative to each other. The rationale of microphotometry is that insertion of the microscope in the optical train, and substitution of a dyed tissue on a slide for a dye solution in a cuvette, does not militate against using Beer’s law to deduce relative concentration of dye molecules from relative absorption of light (Pollister and Ornstein, 1959).
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References
Bachop, W.E., 1965. Size, shape, number, and distribution of periblast nuclei in the muskellunge embryo (Clupeiformes: Esocidae). Trans. Am. microsc. Soc., 84, 80–86.
Bachop, W.E. and Price, J.W., 1971. Giant nuclei formation in the yolk-sac syncytium of the muskellunge, a bony fish (Salmoniformes: Esocidae: Esox masquinongy). J. Morph., 135, 239–246.
Deitch, A.D., 1966. Cytophotometry of nucleic acids, 327–354 pp. In: Introduction to Quantitative Cytochemistry. G.L. Wied (ed.). New York — London: Academic Press, 623 pp.
Garcia, A.M. and Iorio, R., 1966. Potential sources of error in twowave length cytophotometry, 215–237 pp. In: Introduction to Quantitative Cytochemistry. G.L. Wied (ed.). New York — London: Academic Press, 623 pp.
Hale, A.J., 1966. Feulgen microspectrophotometry and its correlation with other cytochemical methods, 183–199 pp. In: Introduction to Quantitative Cytochemistry. G.L. Wied (ed.). New York —London: Academic Press, 623 pp.
Kasten, F.H., 1960. The chemistry of Schiff’s reagent. Intern. Rev. Cytol., X, 1–100.
Korson, R., 1951. A microspectrophotometric study of red cell nuclei during pyknosis. J. exp. Med., 93, 121.
Leuchtenberger, C., 1958. Quantitative determination of DNA in cells by Feulgen microspectrophotometry, 219–278 pp. In: General Cytochemical Methods, Vol. I. J.F. Danielli (ed.). New York — London: Academic Press, 471 pp.
Mendelsohn, M.L., 1958. The two-wave length method of microspectro-photometry. J. biophys. biochem. Cytol., 4, 407–424.
Mendelsohn, M.L. and Richards, B.M., 1958. A comparison of scanning and two-wave length microspectrophotometry. J. biophys. biochem. Cytol., 4, 707–709.
Moses, M., 1952. Quantitative optical techniques in the study of nuclear chemistry. Exp. Cell Res., Suppl., 2 75–102.
Ornstein, L., 1952. Distributional error in microspectrophotometry. Lab. Invest., 1, 250–265.
Patau, K., 1952. Absorption microphotometry of irregular shaped objects. Chromosoma, 5, 341–362.
Patau, K. and Swift, H., 1953. The DNA-content (Feulgen) of nuclei during mitosis in a root tip of onion. Chromosoma, 6, 149–169.
Pearse, A.G.E., 1960. Histochemistry Theoretical and Applied, 2nd ed. Boston: Little Brown and Co., 998 pp.
Pollister, A.W. and Ornstein, L., 1955. Cytophotometric analysis in the visible spectrum, 3–71 pp. In: Analytical Cytology, R.G. Mellors (ed.). New York: McGraw-Hill Book Co.
Pollister, A.W. and Ornstein, L., 1959. The photometric chemical analysis of cells, 431–518 pp. In: Analytical Cytology, 2nd ed., R.G. Mellors (ed.). New York: Mc-Graw-Hill Book Co., 543 pp.
Swift, H. and Rasch, E., 1956. Microphotometry with visible light, 353–400 pp. In: Physical Techniques in Biological Research, Vol. III, G. Oster and A.W. Pollister (eds.). New York — London: Academic Press, 728 pp.
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Bachop, W.E., Schwartz, F.J. (1974). Quantitative Nucleic Acid Histochemistry of the Yolk Sac Syncytium of Oviparous Teleosts: Implications for Hypotheses of Yolk Utilization. In: Blaxter, J.H.S. (eds) The Early Life History of Fish. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65852-5_29
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DOI: https://doi.org/10.1007/978-3-642-65852-5_29
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