Cell Cultures from Tissue Explants

  • Ulrich Wolf


Cell cultures can be prepared from the most diverse tissues and organs. The basic procedure is as follows: Tissue specimens (biopsy or autopsy material) are taken from the body, reduced to small pieces, and set up in vitro in a nutrient medium. There are a number of methods available to prevent expiant fragments from floating in the nutrient medium. In these socalled primary cultures cells divide which are usually morphologically heterogeneous. Depending on the source of material, epithelial or fibroblast-like cells at first predominate. After some time, at the latest after the first subcultures, only fibroblast-like cells are found. When a dense cell layer has formed, the tissue fragments are isolated from the proliferated cells. A subculture is then set up, from which further subcultures are derived after cell multiplication. To obtain mitoses for chromosome analysis, a cell culture in the most vigorous cell growth phase (the logarithmic phase) is processed according to the directions given in Chapter IV.


Cover Slip Fibroblast Culture Culture Bottle Freezing Medium Embryo Extract 
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  1. Böhmer, H. von, Wöhler, W., Wendel, U., Passarge, E., Rüdiger, H. W.: Studies on the optimal cooling rate for freezing human diploid fibroblasts. Exp. Cell Res. 79, 496–498 (1973).CrossRefGoogle Scholar
  2. Evans, V. J., Earle, W. R.: The use of perforated cellophane for the growth of cells in tissue culture. J. nat. Cancer Inst. 8, 103–119 (1947).PubMedGoogle Scholar
  3. Geneva Conference: Standardization of procedures for chromosome studies in abortion. Bull. Wld Hlth Org. 34, 765–782 (1966).Google Scholar
  4. Harnden, D. G.: A human skin culture technique used for cytological examinations. Brit. J. exp. Path. 41, 31 (1960).PubMedGoogle Scholar
  5. Harnden, D. G., Brunton, S.: The skin culture technique. In: Human chromosome methodology (J. J. Yunis, ed.), p. 57–73. New York-London: Academic Press 1965.Google Scholar
  6. Hsu, T. C.: Procedures for mammalian chromosome preparations. In: D. M. Prescott (ed.), Methods in cell physiology, Vol. V, p. 1–36. New York-London: Academic Press 1972.Google Scholar
  7. Hsu, T. C., Kellogg, D. S.: Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens. J. nat. Cancer Inst. 25, 221–235 (1960).PubMedGoogle Scholar
  8. Kruse, P. F., Jr., Patterson, K. M., Jr. (eds.): Tissue Culture. Methods and Applications. New York and London: Academic Press 1973.Google Scholar
  9. Lejeune, J., Gautier, M., Turpin, R.: Les chromosomes humains en culture de tissus. C. R. Acad. Sci. (Paris) 248, 602–603 (1959).Google Scholar
  10. Manojlovic, N., Hienz, H. A.: Zur Methode der primären Explantation und Kultivierung menschlicher Gewebe mit Hilfe von perforiertem Cellophan. Mikroskopie 22, 70–80 (1967).PubMedGoogle Scholar
  11. Merchant, D. J., Kahn, R. H., Murphy, W. H.: Handbook of cell and organ culture, 2nd ed. Minneapolis: Burgess Publ. Co. 1965.Google Scholar
  12. Parker, R. C.: Methods of tissue culture, 3. ed. New York: P. B. Hoeber Inc., Med. Div. Harper Broth. 1961.Google Scholar
  13. Paul, J.: Cell and tissue culture, 4. ed. Edinburgh-London: Livingstone Ltd. 1972.Google Scholar
  14. Penrose, L. S., Smith, G. F.: Down’s anomaly, p. 133ff. London: Churchill Ltd. 1966.Google Scholar
  15. Penso, G., Balducci, D.: Tissue cultures in biological research. Amsterdam-London-New York: Elsevier Publ. Co. 1963.Google Scholar
  16. Puck, T. T., Robinson, A., Tjio, J. H.: Familial primary amenorrhoea due to testicular feminization: a human gene affecting sex differentiation. Proc. Soc. exp. Biol. (N.Y.) 103, 192–196 (1960).Google Scholar
  17. Rothblat, G. H., Cristofalo, V. J. (eds.): Growth, nutrition and metabolism of cells in culture. Vol. I/II. New York and London: Academic Press 1972.Google Scholar
  18. White, P. R.: The cultivation of animal and plant cells, 2nd. New York: Ronald Press 1963.Google Scholar
  19. Willmer, E. N. ed.: Cells and tissues in culture, methods, biology and physiology, vol. I/II/III. London-New York: Academic Press 1965.Google Scholar

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© Springer-Verlag Berlin · Heidelberg 1974

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  • Ulrich Wolf

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