Cell Cultures from Tissue Explants

  • Ulrich Wolf

Abstract

Cell cultures can be prepared from the most diverse tissues and organs. The basic procedure is as follows: Tissue specimens (biopsy or autopsy material) are taken from the body, reduced to small pieces, and set up in vitro in a nutrient medium. There are a number of methods available to prevent expiant fragments from floating in the nutrient medium. In these socalled primary cultures cells divide which are usually morphologically heterogeneous. Depending on the source of material, epithelial or fibroblast-like cells at first predominate. After some time, at the latest after the first subcultures, only fibroblast-like cells are found. When a dense cell layer has formed, the tissue fragments are isolated from the proliferated cells. A subculture is then set up, from which further subcultures are derived after cell multiplication. To obtain mitoses for chromosome analysis, a cell culture in the most vigorous cell growth phase (the logarithmic phase) is processed according to the directions given in Chapter IV.

Keywords

Burner Glycerol Carbide Mercury Foam 

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1974

Authors and Affiliations

  • Ulrich Wolf

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