Abstract
Hershey and Chase demonstrated in 1952 that DNA is the essential component of a phage particle which enters an E. coli cell upon infection with T2. DNA alone initiates and directs the two major reactions in phage infection: DNA replication, and synthesis of phage-specific proteins. Confirming this role of nucleic acids in the infective process, nucleic acids of viruses separated from virus protein were later shown to be infective. This “infection of cells by the isolated nucleic acid from a virus, resulting in the production of complete virus” has been termed transfection (Földes and Trautner, 1964). By this definition any transfection system is distinct from virus infection in that infectivity is sensitive to nucleases, but resistant to antiserum directed against the virus particle. Transfection has been observed and analyzed in a large number of systems. Thus Gierer and Schramm(1956) and Fraenkel-Conrat et al. (1957) demonstrated the infectivity of TMV-RNA, an observation that has since been made for a large number of other plant viruses. Transfection of animal cells by animal virus RNA and DNA has been demonstrated. Bacteriophage nucleic acids isolated from various RNA and DNA phages could be made infective for bacteria. All these findings as well as the discovery of bacterial transformation substantiate the unique role of nucleic acids as genetic material.
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Trautner, T.A., Spatz, H.C. (1973). Transfection in B. subtilis. In: Arber, W., et al. Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung. Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung, vol 62. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65772-6_3
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