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Immunogenicity and Antigenicity of Angiotensin I and II

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Book cover Angiotensin

Abstract

Numerous studies on synthetic homopolymers have indicated a sequence of 6 to 7 residues as the minimal requirement for immunogenicity1, somewhat less being required for antigenicity1, although exceptions such as the series of “arsenyl”- N-acetyltyrosine (MW = 450) have been described (Borek et al., 1965). The small polypeptide hormones comprising the octapeptide Angiotensin II (MW = 1030), the decapeptide Ang. I (MW = 1280), the nonapeptide bradykinin (MW = 1060) and the neurohypophyseal hormones oxytocin, lysine and arginine-vasopressine (MW = 1007 to 1084) are among the smallest natural polypeptides to have immunogenic and/or antigenic properties. These small polypeptide hormones have been used as model haptens for immunochemical studies because they offer the following advantages: a) their primary structure is well established, b) they can be synthesized and are consequently available free of other protein contaminants, c) a large number of analogues are available in which single or multiple amino acids have been exchanged or modified, d) they are easily manipulated chemically, which allows their attachment to carriers or the obtention of well-defined fragments. Each of these polypeptides appears to behave as an integral whole dotted with a defined secondary structure or conformation if not in solution at least in contact with the complementary steric pattern of the antibody’s combining site and of the target cell’s receptor (binding, recognition) site.

Supported by a grant (No. 3.427.70) from the Fonds National Suisse de la Recherche Scientifique.

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  1. Department of Medicine, Hôpital Cantonal, Geneva, Switzerland

    M. B. Vallotton (Assistant Professor of Medicine, Chief of the Endocrine Division)

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  1. The Cleveland Clinic Foundation, 44106, Cleveland, OH, USA

    Irvine H. Page M.D. (Emeritus Director of Research) (Emeritus Director of Research)

  2. Division of Research, The Cleveland Clinic Foundation, 44106, Cleveland, OH, USA

    F. Merlin Bumpus Ph.D. (Chairman) (Chairman)

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Vallotton, M.B. (1974). Immunogenicity and Antigenicity of Angiotensin I and II. In: Page, I.H., Bumpus, F.M. (eds) Angiotensin. Handbuch der experimentellen Pharmakologie / Handbook of Experimental Pharmacology, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65600-2_9

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