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Absorption Spectrophotometry

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Concepts in Biochemical Pharmacology

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Abstract

Absorption spectrophotometry is based on the ability of a substance to absorb radiation, particularly in the wavelength region between 0.2 and 30 microns (µ). Measurement of the degree of absorption at various discrete wavelengths yields a pattern of absorbance relative to wavelength — an absorption spectrum — characteristic of the light-absorbing substance. Such absorption spectra may be used to identify or characterize unknown compounds. In addition, the amount of absorbance of monochromatic light is often proportional to the amount of absorbing substance, thus yielding a method for quantitative estimation of the substance.

Supported in part by USPHS grants MH-14658 and HD-03037 and by Research Development Award KO2-MH-41083 (to R.P. Maickel).

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Authors
  1. R. P. Maickel
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  2. T. R. Bosin
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Editor information

Editors and Affiliations

  1. Department of Health, Education and Welfare, National Institutes of Health, Bethesda, MD, 20014, USA

    Bernard B. Brodie  & James R. Gillette  & 

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© 1971 Springer-Verlag Berlin · Heidelberg

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Maickel, R.P., Bosin, T.R. (1971). Absorption Spectrophotometry. In: Brodie, B.B., Gillette, J.R., Ackerman, H.S. (eds) Concepts in Biochemical Pharmacology. Handbook of Experimental Pharmacology / Handbuch der experimentellen Pharmakologie, vol 28 / 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65177-9_2

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  • DOI: https://doi.org/10.1007/978-3-642-65177-9_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65179-3

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