Abstract
Riboflavin occurs in nature both in the free form, and as the coenzymes riboflavin adenine dinucleotide1 (FAD) and riboflavin mononucleotide1 (FMN, riboflavin-5′-phosphate). Little information is available as to the proportion of the total riboflavin which exists in these various forms in plant tissues. In the rat, Bessey, Lowry and Love (1949) found 70–90% of the total in the form of FAD and only negligible amounts of free riboflavin. The same situation probably holds true for many plant tissues, provided they have not been damaged or allowed to autolyze. For example, in the early days of riboflavin investigations Kuhn, Wagner-Jauregg and Kaltschmitt (1934) observed that over half the riboflavin in spinach leaves was in a bound state, i. e., not diffusable. As is now well known, the flavin coenzymes are more or less firmly bound to specific proteins as essential components of the various yellow enzymes, and most of the riboflavin of intact, metabolically active tissues no doubt exists in these combinations.
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Strong, F.M. (1955). Riboflavin, Folic Acid and Biotin. In: Paech, K., Tracey, M.V. (eds) Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse. Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-64961-5_15
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