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Dotriacontane-16, 17-14C Distribution Pattern in the Respiratory System of Two Hamster Species after Passive Exposure to Radioactive Labelled Smoke

  • Norbert Kmoch
  • U. Mohr

Abstract

The quantitative and qualitative distribution of C14 labeled dotriacontane (DOT-C14) determined by liquid scintillation counting and autoradiography in the respiratory system, the digestive tract, liver and kidneys of Syrian golden and European hamsters, males and females, is described after they had been exposed to radioactive labeled cigarette smoke. The different DOT-C14 distributions are discussed in detail with special attention given to the respiratory tract, related species differences and the topographic subdivisions of apex nasi, fundus nasi, pharynx, larynx, trachea, and lungs. It is apparent that the absolute amount of activity in the respiratory tract related to body size of the Syrian golden hamster is greater than in the European hamster but that the percentual distribution exhibits a greater filtering action of the upper respiratory tract of Syrian golden hamster than of the European hamster so that a larger percentual amount of total inhaled particulate matter reaches the lungs. The European hamster might be a more useful model for the investigation of respiratory tract carcinogenesis due to the possibility of a longer life time exposure and a higher sensitivity to respiratory tract carcinogens.

Keywords

Respiratory Tract Liquid Scintillation Counting Syrian Golden Hamster Smoke Inhalation British American Tobacco 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. CHEVALIER, H.J., DONTENWILL, W.: Experimentelle Untersuchungen über die Ablagerung von inhalierten Partikeln im Kehlkopf von syrischen Goldhamstern. Z. Versuchstierk. 14, 271–276 (1972).Google Scholar
  2. DAVIES, B.R., HOUSEMAN, T.H., RODERICK, H.R.: Studies of cigarette smoke transfer using radioisotopically labeled tobacco constituents. Part III: The use of dotriacontane-16,17-14C as a marker for the deposition of cigarette smoke in the respiratory system of experimental animals. Beitr. Tabakf. 7, 148–153 (1973).Google Scholar
  3. DONTENWILL, W.: Experimental investigations on the effect of cigarette smoke inhalation on small laboratory animals. In: Inhalation Carcinogenesis (M.G. Hanna, Jr., P. Nettesheim, J.R. Gilbert, eds). AEC Symposium Series no. 18, pp. 389–409. Oak Ridge, Tennessee: U.S. Atomic Energy Commission, Division of Technical Inf. 1970.Google Scholar
  4. DONTENWILL, W., CHEVALIER, H.J., HARKE, H.P., LAFRENZ, U., RECKZEH, G., SCHNEIDER, B.: Investigations on the effects of chronic cigarette smoke inhalation in Syrian golden hamsters. J. nat. Cancer Inst. 51, 1781–1832 (1973).PubMedGoogle Scholar
  5. DONTENWILL, W., HARKE, H.P., BAARS, A., GOERTZ, E.: Experimentelle Untersuchungen über Aufnahme und Ablagerung von Rauchbestandteilen bei der passiven Berauchung von Goldhamstern mit Zigarettenrauch. Drug Res. 21, 142–143 (1971).Google Scholar
  6. HOLLAND, R.H., WILSON, R.H., MORRIS, D., MCCALL, M.S., LANZ, H.: The effect of cigarette smoke on the respiratory system of the rabbit. Cancer 11, 709–712 (1958).PubMedCrossRefGoogle Scholar
  7. HOUSEMAN, T.H., HENEAGE, E.: Studies of cigarette smoke transfer using radioisotopically labeled tobacco constituents. Part I: The preparation of radioisotopically labeled cigarettes. Beitr. Tabakforsch. 7, 138–141 (1973).Google Scholar
  8. JENKINS, R.W., Jr., NEWMAN, R.H., CARPENTER, R.D., OSDENE, T.S.: Cigarette smoke formation studies. I. Distribution and mainstream products from added dotriacontane-16,17-14C. Beitr. Tabakforsch. 5, 295–298 (1970).Google Scholar
  9. KAYSER, C.H., AARON, M.: Cycle d’activité saisonnière des glandes endocrines chez un hibernant, le hamster (cricetus frumentarius). C.R. Soc. Biol. (Paris) 125, 225 (1938).Google Scholar
  10. KAYSER, C.H., AARON, M.: Le cycle saisonnière des glandes endocrines chez les hibernants. Arch. Anat. (Strasbourg) 33, 21–40 (1950).Google Scholar
  11. LEWIS, C.I., MCGEADY, J.C., WAGNER, J.R., SCHULTZ, F.J., SPEARS, A.W.: Dichlorobenzophenone as a nonradioactive tracer for cigarette smokegas chromatographic analysis of tracer. Amer. Rev. Resp. Dis. 106, 480–484 (1972).PubMedGoogle Scholar
  12. MOHR, U., ALTHOFF, J., PAGE, N.: Tumors of the respiratory system induced in the common European hamster by N-diethylnitrosamine. J. nat. Cancer Inst. 49, 595–597 (1972).PubMedGoogle Scholar
  13. MOHR, U., REZNIK, G., ECKHART, W., KMOCH, N., HAAS, H.: Distribution of labeled smoke particles in the respiratory tract of the hamster. Naturwissenschaften 61, 133–134 (1974).PubMedCrossRefGoogle Scholar
  14. MOSHY, R.J.: In: Tobacco and Tobacco Smoke (E.L. Wynder and D. Hoffmann, eds), pp. 304–310. New York, London: Academic Press 1967.Google Scholar
  15. PAGE, B.F.Y., WOOLSGROVE, B., CHASSEAUD, L.F., BINNS, R.: Use of radioactive tracer technique in investigations associated with cigarette smoking. Am. Occup. Hyg. 16, 409–416 (1973).CrossRefGoogle Scholar
  16. PATZSCHKE, K.: Ganztier-Autoradiographie. Eine Methode in der Arzneimittelforschung. Münch. med. Wschr. 36, 2043–2053 (1968).Google Scholar
  17. RATHS, P.: Mineralhaushalt und hormonale Aktivität im Winterschlaf. Experientia 20, 178–190 (1964).PubMedCrossRefGoogle Scholar
  18. REZNIK, G., MOHR, U.: A modified method for locating labeled smoke particles in organs of Syrian golden and European hamsters. In: These Proceedings.Google Scholar
  19. STOCKS, P., CAMPBELL, J.M.: Lung cancer death rates among nonsmokers and pipe and cigarette smokers. An evaluation in relation to air pollution by benzpyrene and other substances. Brit. med. J. 2, 923–939 (1955).PubMedCrossRefGoogle Scholar
  20. STOCKS, P.: Recent epidemiological studies of lung cancer mortality, cigarette smoking and air pollution, with discussion of a new hypothesis of causation. Brit. J. Cancer 20, 595–623 (1966).PubMedCrossRefGoogle Scholar
  21. ULLBERG, S.: Studies on the distribution and fate of 35S labeled benzylpenicillin in the body. Acta radiol. (Stockholm) Suppl. 118 (1954).Google Scholar
  22. ZIMMERMAN, K.: In: Taschenbuch unserer wildlebenden Säugetiere, 2nd edition, p. 92. Hannover: Fackelträger 1966.Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1974

Authors and Affiliations

  • Norbert Kmoch
    • 1
  • U. Mohr
    • 1
  1. 1.Abteilung für Experimentelle PathologieMedizinische Hochschule HannoverHannover-KleefeldW.-Germany

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