Abstract
Using labelled proline for investigations on collagen synthesis or its turnover is an effective method, making possible the collection of data by measuring the specific activity of hydroxyproline. Further information is received by fractionation of collagen. Application of these methods was aimed at receiving further information on collagen synthesis and turnover in skin and organs of the intestinal tract.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Beneke, G.: Altersabhängige Veränderungen des Kollagens und der Bindegewebszellen. In: H. Bredt und J.W. Rohen (Eds.): Altern und Entwicklung, S. 1–37. Stuttgart-New York: Schattauer 1971.
Dörmer, P., Brinkmann, W., Stieber, A., Stich, W.: Automatische Silberkornzählung in der Einzelzell-Autoradiographie. Eine neue photometrische Methode für die quantitative Autoradiographie. Klin. Wschr. 44, 477–482 (1966).
Eisen, A.Z., Jeffrey, J.J., Gross, J.: Human skin collagenase. Isolation and mechanism of attack on the collagen molecule. Biochim. biophys. Acta (Amst.) 151, 637–645 (1968).
Gerber, G.B., Gerber, G., Altmann, K.: Some interrelated aspects of proline and hydroxyproline metabolism. Nature 185, 767–768 (1960).
Gerlach, U.: Collagen metabolism. Bibl. anat. (Basel) 496 (1966).
Grasedyck, K., Wulff, U., Rochová, I.: Autoradiographische und radiochemische Befunde zur Kollagensynthese des Magen-Darmtraktes. Verh. dtsch. Ges. Path. 53, 209–215 (1969).
Gries, G., Grasedyck, K.: Über spezifische Kollagenasen in Organen des Magen-Darmtraktes. Verh. dtsch. Ges. Path. 53, 205–209 (1969).
Harris, E.D., Cohen, G.L., Crane, S.M.: Synovial collagenase: its presence in culture from joint disease of diverse etiology. Arthr. and Rheum. 12, 92–102 (1969).
Hauss, W.H., Junge-Hülsing, G., Gerlach, U.: Die unspezifische Mesen-chymreaktion. Zur Pathogenese der reaktiven Mesenchymerkrankungen. Stuttgart: Thieme 1968.
Haust, M.D., More, R.H., Movat, H.Z.: The role of smooth muscle cells in the fibrogenesis of atherosclerosis. Amer. J. Path. 31, 377–389 (1960).
Heikkinen, E.: Transformations of rat skin collagen with special reference to the ageing process. Acta physiol. scand. Suppl. 317 (1968).
Herrmann, R.: Physikalische Grundlagen der Mikroskop-Photometrie. Z. klin. Chem. 8, 321–328 (1970).
Jeffrey, J.J., Gross, J.: Isolation and characterization of a mammalian collagenolytic enzyme. Fed. Proc. 26, 670 (1967).
Junge-Hülsing, G.: Untersuchungen zur Pathophysiologie des Bindegewebes. Habil-Schrift Münster 1963. Bd. 24. Theoretische und klinische Medizin in Einzeldarstellungen. Heidelberg: Hüthig 1965.
Kirschke, H., Hanson, H.: Untersuchungen über den Kollagenabbau durch Rattenorgane. Differenzierung und Charakterisierung Prolinpeptidspaltender Enzyme. Hoppe-Seylers Z. physiol. Chem. 350, 1437–1448 (1969).
Kulonen, E.: Research work on structure and metabolism of collagen. II. Turku: Kirjapaino Polytypos 1971.
Lazarides, E.L., Lukens, L.N., Infante, A.A.: Collagen polysomes: site of hydroxylation of proline residues. J. molec. Biol. 58, 831–846 (1971).
Lazarus, G.S., Decker, J.L., Oliver, C.H., Daniels, J.R., Multz, C.V., Fullmer, H.M.: Collagenolytic activity of synovium in rheumatoid arthritis. N. Engl. J. Med. 279, 914–919 (1968).
Lindner, J.: Zur Physiologie und Pathologie der Schleimbildung des Darmes. Verh. dtsch. Ges. Path. 53, 111–153 (1969).
Lindner, J.: The histochemistry of atherosclerosis. In: F.G. Schettler, G.S. Boyd: Atherosclerosis, p. 73–140. Amsterdam: Elsevier 1969.
Lindy, S., Pedersen, F.B., Turto, H., Uitto, J.: Lactate, lactate dehydrogenase and protocollagen proline hydrolase in rat skin autograft. Hoppe-Seylers Z. physiol Chem., 352, 1113–1118 (1971).
Mee, J.O.D., Rhoads, R.E., Udenfriend, S.: The substrate recognition site of collagen proline hydroxylase: the hydroxylation of -X-Pro-Gly-sequences in bradykinin analogs and other peptides. Arch. Biochem. 144, 343–351 (1971).
Pänkäläinen, M., Aro, H., Simons, K., Kivirikko, K.I.: Protocollagen proline hydroxylase: molecular weight, subunits and isoelectric point. Biochem. biophys. Acta (Amst.) 221, 559–565 (1970).
Prockop, D.J., Udenfriend, S., Lindstedt, S.: A simple technique for measuring the specific activity of labelled hydroxyproline in biological materials. J. biol. Chem. 236, 1395–1398 (1961).
Ross, R., Benditt, E.P.: Wound healing and collagen formation. V. Quantitative electron microscope radioautographic observation of proline 3H utilization by fibroblasts. J. Cell Biol. 27, 83–106 (1965).
Stegemann, H.: Mikrobestimmung von Hydroxyprolin mit Chloramin-T und p-Dimethylaminobenzaldehyd. Hoppe-Seylers Z. physiol. Chem. 311, 41–45 (1958).
Strauch, L., Vencelj, H.: Collagenases in mammalian cells. Hoppe-Seylers Z. physiol. Chem. 348, 465–468 (1967).
Strauch, L., Vencelj, H., Hannig, K.: Kollagenasen in Zellen höher entwickelter Tiere. Hoppe-Seylers Z. physiol. Chem. 349, 171–178 (1968).
Wissler, R.w.: Arterial media cell, smooth muscle or multifunctional mesenchymal cell. J. Atheroscler. Res. 8, 201–213 (1968).
Woods, J.F., Nichols, G.: Distribution of collagenase in rat tissues. Nature 208, 1325–1326 (1965).
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1974 Springer-Verlag Berlin · Heidelberg
About this chapter
Cite this chapter
Grasedyck, K., Wulff, U., Erl, D., Lindner, J. (1974). Studies on Collagen Synthesis Applying Labelled Proline. In: Fricke, R., Hartmann, F. (eds) Connective Tissues. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61932-8_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-61932-8_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-61934-2
Online ISBN: 978-3-642-61932-8
eBook Packages: Springer Book Archive