Mechanisms of Tolerance in Rat Liver Transplantation

  • R. Engemann
  • K. Ulrichs
  • A. Thiede
  • W. Müller-Ruchholtz
  • H. Hamelmann
Conference paper


When the technique for orthotopic rat liver transplantation became available to several microsurgical research groups in the second half of the last decade, it turned out that when inbred strain combinations were used, even with a fully allogeneic donor recipient combination, the animals with grafted livers survived long-term without immunosuppression (Engemann et al. 1982; Houssin et al. 1979; Kamada and Calne 1979; Lie et al. 1980; Limmer et al. 1980; Zimmermann et al. 1979). Moreover, it was more difficult to find a combination in which strong rejection occurred (Engemann et al.1983; Zimmermann et al. 1982). Even though it was well established that tolerance can be induced in rats more easily than in humans, this situation provokes at least the following questions: How does this in vivo finding of long-term graft acceptance correlate with the reactivity in various conventional in vitro assays [mixed leukocyte culture (MLC),microcytotoxicity assay (MCA)]?Does truly specific unresponsiveness develop, and what are the kinetics of unresponsiveness? These questions were investigated at an early point in the recipients’ lives and at two later intervals by skin grafting, MLC, and MCA.


Liver Graft Splenic Lymphocyte Liver Allograft Liver Recipient Cytotoxic Effector Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Deltz E, Ulrichs K, Schack T, Friedrichs B, Thiede A (1983) Verteilungsmuster und Kinetik Cytotoxischer T-Lymphozyten bei florider and supprimierter GVHR nach Dünndarmtransplantation. Langenbecks Arch Chir [Supply Chir Forum] 151–155Google Scholar
  2. Engemann R, Ulrichs K, Thiede A, Müller-Ruchholtz W, Hamelmann H (1982) Value of physiological liver transplant model in rats. Transplantation 33:566–568PubMedCrossRefGoogle Scholar
  3. Engenmann R, Ulrichs K, Deltz e, Thiede A, Müller-Ruchholtz W, Hammelmann H (1983) induction of liver graft tolerance in a primary non tolerant rat starin combination with temporary treatment of cyclosporine. Eur Surg res 15 [Suppl 1]:2986–2991Google Scholar
  4. Houssin D, Gigou M, Franco D, Szekely AM, Bismuth H (1979) Spontaneous long-term survival of liver allografted rats. Transplant Proc 11:567–570PubMedGoogle Scholar
  5. Houssin D, Charpentier B, Lang P, Tamisier D, Gugenheirn J, Gigou M, Bismuth H (1981) In vivo and in vitro correlates of the specific transplantation tolerance induced by spontaneously tolerated liver allografts in inbred strains of rats. Transplant Proc 13:619–622Google Scholar
  6. Kamada N, Calve RY (1979) Orthotopic liver transplantation in the rat. Transplantation 28:47–50PubMedCrossRefGoogle Scholar
  7. Kamada N, Davies HS, Roser B (1981) Reversal of transplantation immunity by liver grafting. Nature 292:840–842PubMedCrossRefGoogle Scholar
  8. Lie TS, Rasche A, Gundermann KJ, Kim YZ, Bernhardt-Huth D (1980) Is the humoral transfer factor responsible for the prolonged survival of rat liver allografts? Eur Surg Res 12 [Suppl 1]:23–24Google Scholar
  9. Lie TS, Galkowska H, Jaeger K, Niehaus KJ (1983) Immunmechanismen nach Lebertransplantation. Langenbecks Arch Chir 360:17–27PubMedCrossRefGoogle Scholar
  10. Limmer J, Herbertson BM, Calve RY (1980) Orthotopic rat liver transplantation using different combinations of four inbred strains. Eur SurgRes 12:343–348PubMedCrossRefGoogle Scholar
  11. Nagao T, White DJG, Calve RY (1982) Kinetics of unresponsiveness induced by a short course of cyclosporin A. Transplantation 33:31–35PubMedCrossRefGoogle Scholar
  12. Strober S, King DP, Gottlieb MS, Hoppe RT, Kaplan HS (1981) Cellular mechanism of tolerance after total lymphoid irradiation (TLI). Transplant Proc 13:556–561PubMedGoogle Scholar
  13. Zimmermann F, Butcher GW, Davies HS, Brons G, Kamada N, Türel O (1979) Techniques for orthotopic liver transplantation in the rat and some studies of the immunologic responses to fully allogeneic liver grafts. Transplant Proc 11:571–577PubMedGoogle Scholar
  14. Zimmermann F, Knoll PP, Davies HS, Gokel JM, Schmid T (1982) The fate of orthotopic liver allografts in different rat strain combinations. Transplant Proc 15:1272–1275Google Scholar

Copyright information

© Springer-Verlag Berlin, Heidelberg 1985

Authors and Affiliations

  • R. Engemann
  • K. Ulrichs
  • A. Thiede
  • W. Müller-Ruchholtz
  • H. Hamelmann

There are no affiliations available

Personalised recommendations