Mechanisms of Tolerance in Rat Liver Transplantation
When the technique for orthotopic rat liver transplantation became available to several microsurgical research groups in the second half of the last decade, it turned out that when inbred strain combinations were used, even with a fully allogeneic donor recipient combination, the animals with grafted livers survived long-term without immunosuppression (Engemann et al. 1982; Houssin et al. 1979; Kamada and Calne 1979; Lie et al. 1980; Limmer et al. 1980; Zimmermann et al. 1979). Moreover, it was more difficult to find a combination in which strong rejection occurred (Engemann et al.1983; Zimmermann et al. 1982). Even though it was well established that tolerance can be induced in rats more easily than in humans, this situation provokes at least the following questions: How does this in vivo finding of long-term graft acceptance correlate with the reactivity in various conventional in vitro assays [mixed leukocyte culture (MLC),microcytotoxicity assay (MCA)]?Does truly specific unresponsiveness develop, and what are the kinetics of unresponsiveness? These questions were investigated at an early point in the recipients’ lives and at two later intervals by skin grafting, MLC, and MCA.
KeywordsLiver Graft Splenic Lymphocyte Liver Allograft Liver Recipient Cytotoxic Effector Cell
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