Rice: Regeneration of Plants from Callus Cultures

Abstract

In callus cultures, shoot meristems and embryo-like structures have often been observed, and these organized structures eventually develop into whole plants. A basic regulatory mechanism underlying organized development involves a balance between auxin and cytokinin. However, the important interactions between plant hormones are still not fully understood. At present, successful aseptic culture and improvement of the culture media and methods permit organ formation in callus cultures from many different plant species. However, it is suggested that various growth regulators must be applied to cells in the right quantity, and sequence, and under the right culture conditions.