Advertisement

Preliminary Characterization of pAT-3, a Symmetry Enhanced Reaction Center Mutant of Rhodobacter capsulatus

  • N. W. Woodbury
  • A. K. Taguchi
  • J. W. Stocker
  • S. G. Boxer
Conference paper
Part of the Springer Series in Biophysics book series (BIOPHYSICS, volume 6)

Abstract

A Rhodobacter capsulatus reaction center mutant has been constructed in which residues M187 through M203 have been replaced by the homologous region of the L polypeptide (L160 through L176). This mutant expresses reaction centers, grows photosynthetically and undergoes electron transfer forming P+QA with high quantum yield. Absorption and Stark measurements indicate a significant alteration in the electronic nature of the special pair, and time-resolved fluorescence measurements suggest changes in the photochemistry of the initial electron transfer reaction. Finally, the redox potential of the special pair has apparently been increased significantly in this mutant to greater than 500 mV.

Keywords

Reaction Center High Quantum Yield Special Pair Midpoint Potential Primary Electron Donor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. J.P. Allen, G. Feher, T. 0. Yeates, H. Komiya, D.C. Rees: Proc. Natl. Acad. Sci. USA 84, 6162–6166 (1987)PubMedCrossRefGoogle Scholar
  2. R.E. Blankenship, W.W. Parson: Biochim. Biophys. Acta 545, 429–444 (1979)PubMedCrossRefGoogle Scholar
  3. E.J. Bylina, C. Kirmaier, L. McDowell, D. Holten, D.C. Youvan: Nature 336, 182–184 (1988)CrossRefGoogle Scholar
  4. E.J. Bylina, S. Ismail, D.C. Youvan: Plasmid 16, 175–181 (1986)CrossRefGoogle Scholar
  5. E.J. Bylina, D.C. Youvan: Proc. Natl. Acad. Sci. USA 85, 7226–7230 (1988)PubMedCrossRefGoogle Scholar
  6. C.H. Chang, D. Tiede, J. Tang, V. Smith, J. Norris, M. Shiffer: FEBS Lett. 205, 82–86 (1986)PubMedCrossRefGoogle Scholar
  7. R.K. Clayton: Photochem. Photobiol. 1, 201–210 (1962)CrossRefGoogle Scholar
  8. J. Diesenhofer, O. Epp, K. Miki, R. Huber, H. Michel: Nature 318, 618–624 (1985)CrossRefGoogle Scholar
  9. J.D. Gust, T.A. Moore, D.K. Luttrull, G.R. Seely, E. Bittersmann, R.V. Bensasson, M. Rougee, E.J. Land, F.C. DeSchryver, M. Van der Auweraer: Photochem. Photobiol. 51, 419–426 (1990)CrossRefGoogle Scholar
  10. C. Kirmaier, D. Holten, W.W. Parson: Biochim. Biophys. Acta 810, 49–61 (1985)CrossRefGoogle Scholar
  11. C. Kirmaier, D. Holten, E.J. Bylina, D.C. Youvan: Proc. Natl. Acad. Sci. 85, 7562–7566 (1988)PubMedCrossRefGoogle Scholar
  12. D. J. Lockhart, S.G. Boxer: Biochemistry 26, 664–668 (1987)CrossRefGoogle Scholar
  13. L.J. Mancino, D.P. Dean, R.E. Blankenship: Biochim. Biophys. Acta 764, 46–54 (1984)CrossRefGoogle Scholar
  14. W.W. Parson: in Photosynthesis (J. Amesz, ed.) Elsevier, Amsterdam (1986)Google Scholar
  15. R.C. Prince, D.C. Youvan: Biochim. Biophys. Acta 890, 286–291 (1987)CrossRefGoogle Scholar
  16. N.W. Woodbury, W.W. Parson: Biochim. Biophys. Acta 850, 197–210 (1986)PubMedCrossRefGoogle Scholar
  17. N.W. Woodbury, E. Bittersmann: in Proceedings of the VIIIth International Congress on Photosynthesis, Stockholm, Sweden (1989) (in press)Google Scholar
  18. D.C. Youvan, S. Ismail, E.J. Bylina: Gene 38, 19–30 (1985)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • N. W. Woodbury
    • 1
  • A. K. Taguchi
    • 1
  • J. W. Stocker
    • 2
  • S. G. Boxer
    • 2
  1. 1.Department of ChemistryArizona State UniversityTempeUSA
  2. 2.Department of ChemistryStanford UniversityCaliforniaUSA

Personalised recommendations