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Structural Analysis and Modifications of the Hairpin Ribozyme

  • Chapter
Book cover Catalytic RNA

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 10))

Abstract

The hairpin ribozyme is a catalytic RNA molecule that has been the subject of increasing interest. The majority of recent work in the hairpin ribozyme system, including that summarized in this review, involves in vitro and cellular studies of trans-acting derivatives (reviewed by Burke 1994). However, it is important to recognize that, in nature, the ribozyme and its target are derived from the negative-polarity strand 359nt satellite RNA associated with tobacco ringspot virus (-)sTRSV and related plant satellite RNA replicons (Prody et al. 1986). The hairpin ribozyme represents the segment of the satellite RNA that contains the activity responsible for site-specific cleavage and ligation reactions required for minus strand replication (Feldstein et al. 1989; Hampel and Tritz 1989; Haseloff and Gerlach 1989). The ribozyme’s substrate is typically an oligonucleotide containing the (-)sTRSV processing site; it contains the sequences that are recognized by the ribozyme. Interestingly, the analogous RNA processing reactions that occur during replication of the plus strand utilize the hammerhead motif (Long and Uhlenbeck 1993). In contrast to phylogenetically abundant RNA catalysts such as self-splicing group I and group II introns, only three naturally occurring variants of the hairpin ribozyme have so far been identified (Rubino et al. 1990). All three are within RNA replicons associated with plant viruses.

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Author information

Authors and Affiliations

  1. Markey Center for Molecular Genetics, Department of Microbiology and Molecular Genetics, The University of Vermont, 306 Stafford Hall, Burlington, VT, 05405, USA

    J. M. Burke, S. E. Butcher & B. Sargueil

Authors
  1. J. M. Burke
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  2. S. E. Butcher
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  3. B. Sargueil
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Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, DD1 4HN, Dundee, UK

    David M. J. Lilley

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© 1996 Springer-Verlag Berlin Heidelberg

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Burke, J.M., Butcher, S.E., Sargueil, B. (1996). Structural Analysis and Modifications of the Hairpin Ribozyme. In: Eckstein, F., Lilley, D.M.J. (eds) Catalytic RNA. Nucleic Acids and Molecular Biology, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61202-2_7

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  • DOI: https://doi.org/10.1007/978-3-642-61202-2_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-62679-4

  • Online ISBN: 978-3-642-61202-2

  • eBook Packages: Springer Book Archive

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