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In Vitro Selection of Catalytic RNA

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Catalytic RNA

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 10))

Abstract

After the first examples of catalytic RNAs raised to prominence earlier proposals that RNA was a likely protagonist in the dawn of evolution, molecular biologists began to ask in earnest about the range of possible RNA activities. This question provided much of the impetus for the development of in vitro RNA selection (Ellington and Szostak 1990; Green et al. 1990; Robertson and Joyce 1990; Tuerk and Gold 1990). With the ability to isolate rare functional individuals from vast pools of sequence variants, investigators have generated molecules that dramatically extend the known range of RNA catalytic and binding activities. The ability to venture further into sequence space has also facilitated study of the sequence and structural requirements of present-day biological RNAs. The technique has recently been extended to the isolation of catalytic DNAs, and may someday generate products useful in clinical, industrial, or research settings.

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Authors and Affiliations

  1. Nine Cambridge Center, Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, USA

    K. P. Williams & D. P. Bartel

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  1. K. P. Williams
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  2. D. P. Bartel
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Editors and Affiliations

  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, DD1 4HN, Dundee, UK

    David M. J. Lilley

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© 1996 Springer-Verlag Berlin Heidelberg

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Williams, K.P., Bartel, D.P. (1996). In Vitro Selection of Catalytic RNA. In: Eckstein, F., Lilley, D.M.J. (eds) Catalytic RNA. Nucleic Acids and Molecular Biology, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61202-2_21

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  • DOI: https://doi.org/10.1007/978-3-642-61202-2_21

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-62679-4

  • Online ISBN: 978-3-642-61202-2

  • eBook Packages: Springer Book Archive

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