Marker-Assisted Genetic Analysis of Winter Hardiness in Barley
A linkage map of barley was developed from 100 doubled haploid lines derived from a winter (Dicktoo) x spring (Morex) barley cross. Based on this map, quantitative trait locus (QTL) analyses were conducted to determine the chromosome location of genes controlling components of winter hardiness. We detected only one major interval on long arm of chromosome 7 accounting for significant QTL effects for winter hardiness. This region was found to be responsible for 39-79% of the phenotypic variation in field survival, 32% of the variation for LT50, and 47% of the variation for growth habit. Two members of the barley dehydrin gene family, Dhn1 and Dhn2, were also found within the region defining the winter hardiness QTL effects. Reasoning that genes controlling winter hardiness may be expressed during cold acclimation, we examined the expression pattern of six barley dehydrin gene family members in shoot tissue in response to cold temperature. Dhn5, located on chromosome 6, was the only dehydrin induced by low temperature under both controlled environments as well as under field conditions. Under these conditions, Dhn1 and Dhn2 were not induced to detectable levels by low temperature. The expression patterns of Dhn5 in shoot tissue for Dicktoo and Morex were identical under the conditions studied, in spite of the known phenotypic differences in their winter hardiness. These results, together with the allelic structure of selected high and low survival doubled haploid lines, suggested that the Dicktoo alleles at the Dhn1 and Dhn2 are probably not the primary determinants of winter hardiness in barley.
KeywordsQuantitative Trait Locus Double Haploid Cold Acclimation Freezing Tolerance Double Haploid Line
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