Abstract
Reconstitution of integral membrane proteins into unilamellar phospholipid vesicles has played and should stay a potentially powerful tool to analyse structural as well as functional areas of membrane protein research. Although it is difficult to list all informations published since the pionering work of Racker and co-v/orkers initiated 20 years ago, a number of reviews are available including specific classes of membrane proteins such as receptors, substrate carriers, energy-conserving enzymes involved in oxidative phosphorylation and ion motive ATPases (Racker, 1979; Eytan, 1982; Casey, 1984; Levitzki, 1985; Jain and Zakim, 1987; Villalobo, 1990; Cornelius, 1991; Rigaud et al, 1995).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
REFERENCES
Bigelow, D.J., and Inesi, G. (1992) Contributions of chemical derivatization and spectroscopic studies to the characterizati on of the Ca transport ATPase of sarcoplasmic reticulum, Biochim. Biophys. Acta, 1113, 323–338.
Bullock, J.O. and Cohen, F.S. (1986) Octylglucoside promotes incorporation of channels into neutral planar phospholipid bilayers. Studies with colicin la, Biochim . Biophys. Acta, 856, 101–108.
Casey, R.P. (1984) Membrane reconstitution of the energy-conserving enzymes of oxidative phosphorylation, Biochim . Biophys. Acta, 768, 319–347.
Cornelius, F (1991) Functional reconstitution of the sodium pump. Kinetics of exchange reactions performed by reconstituted Na /K-ATPase, Biochim . Biophys. Acta, 1071, 19–66.
Chiesi, M., and Inesi, G. (1980) Adenosine 5’-triphosphate dependent fluxes of manganese and hydrogen ions in sarcoplasmic reticulum vesicles. Biochemistry, 19, 2912–2918.
Curman, B., Klareskog, L., and Peterson, P.A. (1980). On the mode of incorporation of human transplantation antigens into lipid vesicles, J. Biol. Chem., 255, 7820–7826.
Eytan, G.D.(1982) Use of liposomes for reconstitution of biological functions, Biochim . Biophys. Acta, 694, 185–202.
Graber, P. (1990) Kinetics of proton transport coupled ATP synthesis in chloroplasts. Bioelectrochemistry III, Milazzo, G. and Blank, M., Plenum Press, New York, 277–310.
Hao, L., Rigaud, J.L., and Inesi, G. (1994) Ca /H coutertransport and electrogenicity in proteoliposomes containing erythrocyte plasma membrane Ca -ATPase and exogenous lipids. J. Biol.Chem. 269, 14268–14275
Haynes, D.H.(1982) Relationships between H~, anion and monovalent cation movements and Ca++ transport in sarcoplasmic reticulum: further proof for a cation exchange mechanism for the Ca -ATPase pump. Arch. Biochem.Biophys., 215, 444–461.
Helenius, A. and Simons, K.(1975) Solubilization of membranes by detergents, Biochim. Biophys. Acta, 415, 29–79.
Helenius, A., Sarvas, M. and Simons, K. (1981) Asymmetric and symmetric membrane reconstitution by detergent elimination, Eur. J. Biochem., 116, 27–35.
Holloway, P.W. (1973) A simple procedure for removal of triton X-100 from protein samples, Analytical Biochemistry, 53, 304–307.
Jain, M.K. and Zakim, D.(1987) The spontaneous incorporation of proteins into preformed bilayers, Biochim. Biophys. Acta, 906, 33–68.
Klein, U., and Farenholz, F. (1994) Reconstitutio n of the myometrial oxytocin receptor into proteoliposomes. Eur. J. Biochem., 220, 559–567.
Kramer, R., and Heberger, C. (1986) Functional reconstitution of carrier proteins by removal of detergent with hydrophobic ion exchange column. Biochim. Biophys. Acta, 863, 289–296.
Lasic, D. (1988) The mechanism of vesicle formation, Biochem. J., 256, 1–11.
Levitzki, A. (1985) Reconstitution of membrane receptor systems, Biochim. Biophys. Acta, 822, 127–153.
Levy, D., Gulik, A., Seigneuret, M. and Rigaud, J.L (1990, a ) Phospholipid vesicle solubilization and reconstitution by detergent. Symmetrical analysis of the two processes using octaethylene glycol moric»-N-dodecyl ether (C12E8), Biochemistry, 29, 480–9488.
Levy, D., Bluzat, A., Seigneuret, M. and Rigaud, J.L. (1990, b) A systematic study of liposome and proteoliposome reconstitution involving Bio-Bead-mediated TX-100 removal, Biochim. Biophys. Acta, 1025, 179–190.
Levy, D., Seigneuret, M., Bluzat, A. and Rigaud, J.L. (1990, c) Evidence for proton coutertransport by the sarcoplasmic reticulum Ca -ATPase during calcium transport in reconstituted proteoliposomes with ionic permeability, J. Biol. Chem., 265,19524–19534.
Levy, D., Gulik, A Bluzat, A. and Rigaud, J.L. (1992) Reconstitution of the sarcoplasmic reticulum Ca ATPase: mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents, Biochim. Biophys. Acta, 1107, 283–298.
Lichtenberg, D., Robson, R.J. and Dennis, E.A. (1983) Solubilization of phospholipids by detergents. Structural and kinetic aspects, Biochim. Biophys. Acta, 737, 285,
Madeira, V.M.C. (1978) Proton gradient formation during transport of Ca++ by sarcoplasmic reticulum. Arch.Biochem.Biophys., 185, 316–325.
Oesterhelt, D., Tittor, J. and Bamberg, E. (1992) A unifying concept for ion translocation by retinal proteins, Journal of Bioenergetics and Biomembranes, 24, 181–191.
Patemostre, M.T., Roux, M. and Rigaud, J.L. (1988) Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 1. Solubilization of large unilamellar liposomes (prepared by reverse-phase evaporation) by triton X-100, octyl glucoside and sodium cholate, Biochemistry, 29, 2668–2677.
Racker, E.(1979) Reconstitution of membrane processes, Methods in Enzymology, 55, 699–711.
Richard, P., Rigaud, J.L. and Graber, P.(1990) Reconstitution of CF0F1 into liposomes using a new reconstitution procedure, Eur. J. Biochem, 193, 921–925.
Richard, P., Pitard, B. and Rigaud, J.L. (1995) ATP synthesis by the F0F1 ATPase from the thermophilic Bacillus PS3 co-reconstituted with bacteriorhodopsin into liposomes. J.Biol. Chem., in press
Rigaud, J.L. Bluzat, A. and Biischlen, S. (1983) Incorporation of bacteriorhodopsin into large unilamellar liposomes by reverse phase evaporation, Biochem. Biophys. Res. Commun., 111,373–382.
Rigaud, J.L., Patemostre, M.T. and Bluzat, A. (1988) Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 2. Incorporation of the light-driven proton pump bacteriorhodopsin, Biochemistry, 27, 2677–2688.
Rigaud, J.L, Pitard, B. and Levy, D. (1995) Reconstitution of membrane proteins into liposomes: application to energy transducing membrane proteins. Biochim.Biophys. Acta, reviews in Bioenergetic, in press.
Seigneuret, M. and Rigaud, J.L. (1985) Use of the fluorescent pH probe pyranine to detect heterogeneous directions of proton movement in bacteriorhodopsin reconstituted large liposomes, FEBS, 188, 101–106.
Seigneuret, M. and Rigaud, J.L. (1986, a ) Analysis of passive and light-driven ion movements in large bacteriorhodospin liposomes reconstituted by reverse-phase evaporation. 1. Factors governing the passive proton permeability of the membrane, Biochemistry, 25, 6716–6723.
Seigneuret, M. and Rigaud, J.L. (1986, b) Analysis of passive and light-driven ion movements in large bacteriorhodopsin liposomes reconstituted by reverse-phase evaporation. 2. influence of passive permeability and back-pressure effects upon light-induced proton uptake, Biochemistry, 25, 6723–6729.
Seigneuret, M. and Rigaud, J.L. (1988) Partial separation of inwardly pumping and outwardly pumping bacteriorhodospin reconstituted liposomes by gel filtration, FEBS, 228, 79–84.
Silvius, J.R. (1992) Solubilization and functional reconstitution of biomembrane components, Annu. Rev. Biophys. Biomol. Struct, 21., 323–348.
Ueno, T. and Sekine, T. (1981) A role of H+ flux in active Ca++ transport into SR vesicles. I. Effect of an artificially imposed H+ gradient on Ca++ uptake. J.Biochem., 89, 1239–1246.
Villalobo,A. (1990) Reconstitution of ion-motive transport ATPases in artificial lipids membranes. Biochim. Biophys. Acta, 1017, 1–48.
Yamaguchi, M. and Kanazawa, T. (1985) Coincidence of H+ binding and Ca++ dissociation in the sarcoplasmic reticulum Ca++ ATPase during ATP hydrolysis. J.Biol.Chem., 260, 4896–4900.
Yu, X., Hao, L. and Inesi, G. (1994) A pK change of acidic residues contributes to cation countertransport in the Ca-ATPase of sarcoplasmic reticulum. J.Biol.Chem., 269, 16656–16661.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Rigaud, JL., Levy, D. (1996). A New Efficient Strategy to Reconstitute Membrane Proteins into Liposomes : Application to the Study of Ca++-Atpases. In: Op den Kamp, J.A.F. (eds) Molecular Dynamics of Biomembranes. NATO ASI Series, vol 96. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61126-1_30
Download citation
DOI: https://doi.org/10.1007/978-3-642-61126-1_30
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-64707-9
Online ISBN: 978-3-642-61126-1
eBook Packages: Springer Book Archive