Skip to main content
SpringerLink
Log in

Lipid-protein interactions in chloroplast protein Import

  • Conference paper
Molecular Dynamics of Biomembranes

Part of the book series: NATO ASI Series ((ASIH,volume 96))

Abstract

Proteins function at distinct sites in a cell, yet they are primarily synthesized in one compartment, the cytosol. (For a concise collection of reviews see (Neupert and Lill, 1992)). This implies that newly synthesized proteins have to be transported to their final destination. Protein transport often involves membrane passage which is a fascinating event because large often polar polypeptide chains of variable composition have to move through an ultra-thin membrane without compromising the other essential barrier function of that membrane.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Billecocq, A (1974) Structures of biological membranes: Localization of galactosyldiglycerides in chloroplasts by means of specific antibodies. Biochim Biophys Acta 352: 245–251

    Article  PubMed  CAS  Google Scholar 

  • Bulychev, A, Pilon, M, Dassen, H, Van’t Hof, R, Vredenberg, W & De Kruijff, B (1994) Precursormediated opening of translocation pores in chloroplast envelopes. FEBS Lett 356: 204–206

    Article  PubMed  CAS  Google Scholar 

  • Chupin, V, Van’t Hof, R & De Kruijff, B (1994) The transit sequence-dependent binding of the chloroplast precursor protein ferredoxin to lipid vesicles and its implications for membrane stability. FEBS Lett 350: 104–108

    Article  PubMed  CAS  Google Scholar 

  • Cornwell, KL & Keegstra, K (1987) Evidence that a chloroplast surface protein is associated with a specific binding site for the precursor to the small subunit of ribulose-l,5-biphosphate carboxylase. Plant Physiol 85: 780–785

    Article  PubMed  CAS  Google Scholar 

  • Cull is, PR & De Kruijff, B (1979) Lipid polymorphism and the functional roles of lipids in biological membranes. Biochim Biophys Acta 559: 399–420

    PubMed  CAS  Google Scholar 

  • De Boer, AD & Weisbeek, PJ (1991) Chloroplast topogenesis: Protein import, sorting and assembly.Biochim Biophys Acta 1071: 221–253

    Google Scholar 

  • Demel, RA, De Swaaf, ME, Mannock, D, Van’t Hof, R & De Kruijff, B (1995) The specificity of glycolipid-preferredoxin interaction. Mol MembrBiol 12: 255–261

    Article  CAS  Google Scholar 

  • Dome, A-J, Joyard, J, Block, MA & Douce, R (1985) Do thylakoids really contain phosphatidylcholine. Proc Natl Acad Sci USA 87: 71–74

    Google Scholar 

  • Douce, R, Block, MA, Dome, A-J & Joyard, J (1984) The plastid envelope membranes: their structure, composition and role in chloroplast biogenesis. Subcell Biochem 10: 1–84

    Article  PubMed  CAS  Google Scholar 

  • Douce, R & Joyard, J (1990) Biochemistry and function of the platid envelope. Annu Rev Cell Biol 6: 173–216

    Article  PubMed  CAS  Google Scholar 

  • Douglas, MG, McCammon, MT & Vassaroti, A (1986) Targeting proteins into mitochondria. Microbiol Rev 50: 166–178

    PubMed  CAS  Google Scholar 

  • Eilers, M & Schatz, G (1986) Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria. Nature 322: 228–232

    Article  PubMed  CAS  Google Scholar 

  • Ellis, RJ (1981) Chloroplast proteins: synthesis, transport and assembly. Annu Rev Plant Physiol 32: 111–137

    Article  CAS  Google Scholar 

  • Endo, T, Kawamura, M & Nakai, M (1992) The chloroplast-targeting domain of a plastocyanin transit peptide can form a helical structure but does not have a high affinity for lipid bilayers. Eur J Biochem 207:671–675

    Article  PubMed  CAS  Google Scholar 

  • Friedman, AL & Keegstra, K (1989) Quantitative analysis of precursor binding. Plant Physiol 89: 993–-999

    Article  PubMed  CAS  Google Scholar 

  • Gasser, SM, Daum, G & Schatz, G (1982) Import of proteins into mitochondria. Energy-dependent uptake of precursors by isolated mitochondria. J Biol Chem 257, 13034–13041

    CAS  Google Scholar 

  • Gierash, LM (1989) Signal sequences. Biochemistry 28, 923–930

    Article  Google Scholar 

  • Hageman, J, Baecke, C, Ebskamp, M, Pilon, M Smeekens, S & Weisbeek, PJ (1990) Protein import into and sorting inside the chloroplast are independent processes. Plant Cell 2: 479–494

    Article  PubMed  CAS  Google Scholar 

  • Hall, PO & Rao, KK (1977) in: Encyclopedia of Plant Physiology 5: Pirson A and Zimmerman MH, Eds 206-215

    Google Scholar 

  • Hall, DQ & Rao, KK (1987) Photosynthesis 4th Ed Edward Arnold Ltd, London

    Google Scholar 

  • Hay, R, Bahni, P & Gasser, S (1984) How mitochondria import proteins. Biochim Biophys Acta 779: 65–87

    PubMed  CAS  Google Scholar 

  • Hirsch S, Michael E, Heemeyer F, Von Heijne G and Soli J (1994) A receptor component of the chloroplast protein translocation machinery. Science 266: 1989–1992

    Article  PubMed  CAS  Google Scholar 

  • Homiak, L, Pilon, M, Van’t Hof, R & De Kruijff, B (1993) The secondary structure of the ferredoxin transit sequence is modulated by its interaction with negatively charged lipids. FEBS Lett 334: 241 — 246

    Article  Google Scholar 

  • Hovius, R, Lambrechts, H, Nicolay, K & De Kruijff, B (1990) Improved methods to isolate and subfractionate rat liver mitochondria. Lipid composition of the inner and outer membrane. BBA 1021: 217–226

    Article  PubMed  CAS  Google Scholar 

  • Howard, KP & Prestigard, JH (1995) Membrane and solution conformations of monogalactosyldiacylglycerol using NMR/molecular modeling methods. J Am Chem Soc 117: 5031–5040

    Article  CAS  Google Scholar 

  • Keegstra, K (1989) Transport and routing of proteins into chloroplasts. Cell 56, 247–253

    Article  PubMed  CAS  Google Scholar 

  • Kessler, F, Blobel, G, Patel, HA & Schnell, DJ (1994) Identification of two GTP-binding proteins in the chloroplast protein import machinery. Science 266: 1035–1039

    Article  PubMed  CAS  Google Scholar 

  • Lancelin, J-M, Bally, I, Arland, GJ, Blackedge, M, Gans, P, Stein, M & Jacquot, J-P (1994) NMR structure of ferredoxin chloroplastic transit peptide from Chlamydomonas reinhardtii promoted by trifluoroethanol in aqueous solution. FEBS Lett 350: 104–108

    Article  Google Scholar 

  • MĂĽller, G & Zimmermann, R (1988) Import of honeybee prepromelittin into the endoplasmic reticulum: energy requirements for membrane insertion. EMBOJ 7: 639–648

    PubMed  Google Scholar 

  • Neupert, W & Lill, R (1992) Elsevier, Amsterdam. Membrane biogenesis and protein targeting

    Google Scholar 

  • Olsen, LJ, Theg, SM, Selman, BR & Keegstra, K (1989) ATP is required for the binding of precursor proteins to chloroplasts. J Biol Chem 264: 6724–672

    PubMed  CAS  Google Scholar 

  • Perry, SE & Keegstra, K (1994) Envelope membrane proteins that interact with chloroplast precursor proteins. The plant cell 6: 93–105

    Article  PubMed  CAS  Google Scholar 

  • Pilon, M, de Kruijff, B & Weisbeek, PJ (1992) New insights into the import mechanism of the ferredoxin precursor into chloroplasts. J Biol Chem 267: 2548—2556

    PubMed  Google Scholar 

  • Pilon, M, Rietveld, AG, Weisbeek, PJ & de Kruijff, B (1992) Secondary structure and folding of a functional chloroplast precursor protein. J Biol Chem 267: 19407—19413

    Google Scholar 

  • Pilon, M, Weisbeek, PJ & De Kruijff, B (1992) Kinetic analysis of translocation into isolated chloroplasts of the purified ferredoxin precursor. FEBS Lett 302: 65–68

    Article  PubMed  CAS  Google Scholar 

  • Pilon, M, Wienk, H, Sips, W, De Swaaf, ME, Talboom, F, Van ’t Hof, R, De Korte-Kool, G, Weisbeek, PJ & De Kruijff, B (1995) Functional domains of the ferredoxin transit sequence involved in chloroplast import. J Biol Chem 270: 3882–3893

    Article  PubMed  CAS  Google Scholar 

  • Biochem Rapoport, TA (1992) Transport of proteins across the endoplasmic reticulum membrane. Science 258:931–936

    Article  PubMed  CAS  Google Scholar 

  • Rietveld, AG, Koorengevel, MC & De Kruijff, B (1995) Non-bilayer lipids are required for efficient protein transport across the plasma membrane of Escherichia coli. EMBO J: in press

    Google Scholar 

  • Robinson, G & Ellis, RJ (1984) Transport of proteins into chloroplasts. Partial purification of a chloroplast protease involved in the processing of imported precursor polypeptides. Eur J 142: 337–342

    CAS  Google Scholar 

  • Roise, D & Schatz, G (1988) Mitochondrial presequences. J Biol Chem 263: 4509–4511

    PubMed  CAS  Google Scholar 

  • Sanders, SL & Schekman, R (1992) Polypeptide translocation across the endoplasmic reticulum membrane. J Biol Chem 267, 13791–13794

    PubMed  CAS  Google Scholar 

  • Schleyer, M, Schmidt, B & Neupert, W (1982) Requirement of a membrane potential for the posttranslational transfer of proteins into mitochondria. Eur J Biochem 125: 109–-116

    Article  PubMed  CAS  Google Scholar 

  • Schnell, DJ, Blobel, G & Pain, D (1990) The chloroplast import receptor is an integral membrane protein of chloroplast envelope contact sites. J Cell Biol 111: 1825–1838

    Article  PubMed  CAS  Google Scholar 

  • Schnell, DJ, Kessler, F & Blobel, G (1994) Isolation of components of the chloroplast protein import machinery. Science 266: 1007–1012

    Article  PubMed  CAS  Google Scholar 

  • Seedorf, M & Soil, J (1995) Copper chloride, an inhibitor of protein import into chloroplasts. FEBS Lett 367: 19–22

    Article  PubMed  CAS  Google Scholar 

  • Shipley, GG, Green, JP & Nichols, BW (1973) The phase behavior of monogalactosyl, digalactosyl and sulphoquinovosyl. Biochem Biophys Acta 311: 531—544

    PubMed  Google Scholar 

  • Smeekens, S, van Binsbergen, J & Weisbeek, PJ (1985) The plant ferredoxin precursor: nucleotide sequence of a full length cDNA done. Nucleic Acid Res 13: 3179–3194

    Article  PubMed  CAS  Google Scholar 

  • Smeekens, S, van Steeg, H, Bauerle, C, Bettenbroek, H, Keegstra, K & Weisbeek, PJ (1987) Import into chloroplasts of a yeast mitochondrial protein directed by ferredoxin and plastocyanin transit peptides. Plant Mol Biol 9: 377–388

    Article  CAS  Google Scholar 

  • Sugiura, M (1992) The chloroplast genome. Plant Mol Biol 19: 149–168

    Article  PubMed  CAS  Google Scholar 

  • Theg, SM, Bauerle, C, Olsen, LJ, Selman, BR & Keegstra, K (1989) Internal ATP is the only energy requirement for the translocation of precursor proteins across chloroplastic membranes. J BiolChem 264: 6730–6736

    CAS  Google Scholar 

  • Umesono, K & Ozeki, H (1987) Chloroplast gene organization in plants. Trends Genet 3: 281—287

    Article  Google Scholar 

  • Van’t Hof, R, Demel, RA, Keegstra, K & De Kruijff, B (1991) Lipid-peptide interactions between fragments of the transit peptide of ribulose-l,5-bisphosphate carboxylase/oxygenase and chloroplast membrane lipids. FEBS Lett 291: 350–354

    Article  Google Scholar 

  • Van ’t Hof, R, Van Klompenburg, W, Pilon, M, Kozubek, A, De Korte-Kool, G, Demel, RA, Weisbeek, PJ & De Kruijff, B (1994) The transit sequence mediates the specific interaction of the precursor of ferredoxin with chloroplast envelope membrane lipids. J Biol Chem 268: 4037—4042

    Google Scholar 

  • Van’t Hof, R & De Kruijff, B (1995) Transit sequence-dependent binding of the chloroplast precursor protein ferredoxin to lipid vesicles and its implications for membrane stability. FEBS Lett 356: 204— 206

    Google Scholar 

  • Van’t Hof, R & De Kruijff, B (1995) Characterization of the import process of a transit peptide into chloroplasts. J Biol Chem (in press)

    Google Scholar 

  • Von Heijne, G, Steppuhn, J & Herrmann, RG (1989) Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem 180: 535–545

    Article  Google Scholar 

  • Von Heijne, G & Nishikawa, K (1991) Chloroplast transit peptides. The perfect random coil?FEBS Lett 278: 1–3

    Article  Google Scholar 

  • Waegemann, K, Paulsen, H & Soil, J (1990) Translocation of proteins into isolated chloroplasts requires cytosolic factors to obtain import competence. FEBS Lett 261: 89–92

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry of Membranes, Center for Biomembranes and Lipid Enzymology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584, CH Utrecht, The Netherlands

    Ben de Kruijff

  2. Department of Molecular and Cell Biology, University of California, Berkeley, USA

    Rien Pilon

  3. Center for Protein Technology, TNO/WAU, The Netherlands

    Ron Van’t Hof & Rudy Demel

Authors
  1. Ben de Kruijff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rien Pilon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ron Van’t Hof
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rudy Demel
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Centre for Biomembranes and Lipid Enzymology, Institute of Biomembranes Utrecht University, Padualaan 8, 3584, CH Utrecht, The Netherlands

    Jos A. F. Op den Kamp

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

de Kruijff, B., Pilon, R., Van’t Hof, R., Demel, R. (1996). Lipid-protein interactions in chloroplast protein Import. In: Op den Kamp, J.A.F. (eds) Molecular Dynamics of Biomembranes. NATO ASI Series, vol 96. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61126-1_10

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-61126-1_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64707-9

  • Online ISBN: 978-3-642-61126-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation