Abstract
Embryonic stem (ES) cell lines isolated from the inner cell mass of the mouse blastocyst (Evans & Kaufman, 1983), can be maintained as pluripotent stem cells and genetically manipulated in vitro. When reintroduced into a host embryo they can contribute to all tissues, including the germline of the resultant chimera (Beddington & Robertson, 1989). ES cells have provided the essential tool in the genetic manipulation of the mouse genome whereby an alteration to an allele of a loci can be made in vitro, introduced into the germline and then bred to homozygosity where the phenotypic effect can be examined.
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© 1996 Springer-Verlag Berlin Heidelberg
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Forrester, L., Joyner, A., Bernstein, A., Wurst, W. (1996). Directed Gene Trapping in Embryonic Stem Cells. In: Zander, A.R., Ostertag, W., Afanasiev, B.V., Grosveld, F. (eds) Gene Technology. NATO ASI Series, vol 94. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61122-3_9
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DOI: https://doi.org/10.1007/978-3-642-61122-3_9
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