Abstract
The hematopoietic system can be considered as a spectrum of differentiation and self renewal with at one extreme terminally differentiated cells such as erythrocytes or macrophages, and at the other, pluripotential hematopoietic stem cells (HSC), which can give rise to all the different lineages of the hematopoietic system, and due to their capacity for self renewal, persist throughout adult life (Spangrude et al. 1991). Introduction of limiting numbers of these cells into recipients whose own hematopoietic systems have been ablated (by, for example, irradiation) results in long-term reconstitution by the donor cells. These properties of HSC have make them tempting targets for gene manipulation, both for investigating the molecular and cellular biology of genes that are expressed in different lineages of the hematopoietic system and in somatic gene therapy. In the former case, gene manipulation of HSC would have particular benefit where the mutations, if transmitted through the germ line, would result in embryonic lethality
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© 1996 Springer-Verlag Berlin Heidelberg
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Hole, N. et al. (1996). Hematopoietic Differentiation of Embryonal Stem Cells in vitro.. In: Zander, A.R., Ostertag, W., Afanasiev, B.V., Grosveld, F. (eds) Gene Technology. NATO ASI Series, vol 94. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61122-3_1
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DOI: https://doi.org/10.1007/978-3-642-61122-3_1
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