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Introduction to High-Speed Flow Sorting

  • Conference paper
Flow and Image Cytometry

Part of the book series: NATO ASI Series ((ASIH,volume 95))

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Abstract

Purification of viable cells by flow cytometric cell sorting, initiated in the mid sixties, has become standard procedure in a variety of research fields. Single cell suspensions, which are stained with fluorochromes to distinguish desired from undesired cells, can be sorted into two purified populations. Sorts can be performed under sterile conditions and usually the viability of the sorted cells is excellent.

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References

  • Alberti S, Stovel R, Herzenberg LA: Preservation of cells sorted individually onto microscope slides with a fluorescence–activated cell sorter. Cytometry 5:644–647, 1984.

    Article  PubMed  CAS  Google Scholar 

  • Bakker Schut TC, de Grooth BG, Greve J: A new principle of cell sorting by using selective electroporation in a modified flow cytometer. Cytometry 11:659–66, 1990.

    Article  PubMed  CAS  Google Scholar 

  • Berenson RJ, Bensinger WI, Hill RS, Andrews RG, Garcia-Lopez J, Kalamansz DF, Still BJ, Spitzer G, Buckner CD, Bernstein ID, Thomas ED: Engraftment after infusion of CD34+ marrow cells in patients with breast cancer of neuroblastoma. Blood 77:1717–1722, 1991.

    PubMed  CAS  Google Scholar 

  • Betz JW, Aretz W, Härtel W: Use of flow cytometry in industrial microbiology for strain improvement programs. Cytometry 5:145–150, 1984.

    Article  PubMed  CAS  Google Scholar 

  • Dangl JL, Parks DR, Oi VT, Herzenberg LA: Rapid isolation of cloned isotype switch Variants variants using fluorescence activated cell sorting. Cytometry 2:395–401, 1982.

    Article  PubMed  CAS  Google Scholar 

  • Dubelaar GJB, Groenewegen AC, Stokdijk W, Van den Engh GJ, Visser JWM: Optical Plankton Analyzer: a flow cytometer for plankton analysis, II: specifications. Cytometry 10: 529–539, 1989.

    Article  PubMed  CAS  Google Scholar 

  • Dühnen J, Stegeman J, Wiezorek C and Mertens H: A new fluid switching flow cytometer. Histochemistry 77:117–121, 1983.

    Article  PubMed  Google Scholar 

  • Farmer C, Esposito R, Van den Engh G: High speed chromosome sorting with a “jet in air” nozzle. Cytometry Supplement 5:116, 1991.

    Google Scholar 

  • Friedman M: Digital fluidic amplifier particle sorter. U.S. Patent 3,791,517, 1974.

    Google Scholar 

  • Fulwyler MJ: Electronic separation of biological cells by volume. Science 150:910– 911,1965.

    Article  PubMed  CAS  Google Scholar 

  • Galbraith DW, Harkins KR, Jefferson RA: Flow cytometric characterization of the chlorophyll contents and size distributions of plant protoplasts. Cytometry 9:75–83, 1988.

    Article  PubMed  CAS  Google Scholar 

  • Herweijer H, Stokdijk W, Visser JWM: High-speed photodamage cell selection using bromodeoxyuridine/Hoechst 33342 photosensitized cell killing. Cytometry 9:143– 149, 1988.

    Article  PubMed  CAS  Google Scholar 

  • Herweijer H: Photodamage cell selection: Development of a high-speed cell selection method based on flow cytometry. Thesis, University of Leiden, Leiden, The Netherlands, 1988.

    Google Scholar 

  • Johnson LA, Flook JP, Hawk HW: Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Biol Reprod 41:199–203, 1989.

    Article  PubMed  CAS  Google Scholar 

  • Kamenski LA and Melamed MR: Spectrometric cell sorter. Science 156:1364–1365, 1967.

    Article  Google Scholar 

  • Keij JF, Groenewegen AC, Stokdijk W, Visser JWM: Coincidence in high-speed flow cytometry: models and measurements. Cytometry 12:398–404, 1991.

    Article  PubMed  CAS  Google Scholar 

  • Keij JF, Groenewegen AC, Visser JWM: High-speed photodamage cell sorting: an evaluation of the ZAPPER prototype. In: Methods in Cell Biology, VOL. 42. Darzynkiewicz Z, Robinson JP, Crissman HA (eds.). Academic Press, San Diego, 1994, pp 371–386.

    Google Scholar 

  • Keij JF, Groenewegen AC, Dubelaar GBJ, Visser JWM:High-speed photodamage cell selection using a frequency doubled argon ion laser. Cytometry 19:209–216, 1995.

    Article  PubMed  CAS  Google Scholar 

  • Leary JF, Ellis SP, McLaughlin SR, Corio MA, Hespelt S, Gram JG, Burde S: High-resolution separation of rare cell types. In: Cell Separation Science and Technology (ACS symposium series 464). Kompala DS and Todd P (eds). ACS, Washington, 1991, pp 26–40.

    Chapter  Google Scholar 

  • Leary JF: Strategies for rare cell detection and isolation. In: Methods in Cell Biology, VOL. 42. Darzynkiewicz Z, Robinson JP, Crissman HA (eds.). Academic Press, San Diego, 1994, pp 331–358.

    Google Scholar 

  • Lindmo T, Fundingsrud K: Measurement of the distribution between cell passages in flow cytometry as a method for the evaluation of sample preparation procedures. Cytometry 2, 151–154, 1981.

    Article  PubMed  CAS  Google Scholar 

  • Martin JC, Jett JH: Photodamage, a basis for super high speed cell selection Cytometry 2:114, 1981.

    Google Scholar 

  • Morrell JM, Keeler KD, Noakes DE, Mackenzie NM, Dresser DW: Sexing of sperm By flow cytometry. Veterinary Record 122:322–324, 1988.

    Article  PubMed  CAS  Google Scholar 

  • McCoy JP, Chambers WH, Lakomy R, Campbell JA, Stewart CC: Sorting minor subpopulations of cells: use of fluorescence as the triggering signal. Cytometry 12:268–274, 1991.

    Article  PubMed  Google Scholar 

  • Peeters JCH, Dubelaar GJB, Ringelberg J, Visser JWM: Optical Plankton Analyzer: a flow cytometer for plankton analysis, I: design considerations. Cytometry 10:522– 528, 1989.

    Article  PubMed  CAS  Google Scholar 

  • Peters D, Branscomb E, Dean P, Merrill T, Pinkel D, Van Dilla M, Gray J: The LLNL high-speed sorter: design features, operational characteristics, and biological utility. Cytometry 6:290–301, 1985.

    Article  PubMed  CAS  Google Scholar 

  • Shah PD, Keij J, Gilbertson SM, Rowley DA: Thy-1+ and Thy-1- natural killer cells. J Exp Med. 163:1012–1017, 1986.

    Article  PubMed  CAS  Google Scholar 

  • Shapiro HM: Apparatus and method for killing unwanted cells. U.S. Patent 4,395,397, 1983.

    Google Scholar 

  • Singer CRJ, Linch DC, Bown SG, Huehns ER, Goldstone AH: Differential phtalocyanine photosensitization of acute myeloid leukaemia progenitor cells: a potential purging technique for autologous bone marrow transplantation. Brit J Haematol 68:417–422, 1988.

    Article  CAS  Google Scholar 

  • Sweet RG: Flow sorter for biological cells. In: Flow Cytometry and Sorting. Melamed MR, Mullaney PF and Mendelson ML (eds). Wiley, New York, 1979, pp. 177–189.

    Google Scholar 

  • Upreti GC, Riches PC, Johnson LA: Attempted sexing of bovine spermatozoa by fractionation on a percoll density gradient. Gemete Research 20:83–92, 1988.

    Article  CAS  Google Scholar 

  • Van den Engh G, Stokdijk W: Parallel processing data acquisition system for Multilaser multilaser flow cytometry and cell sorting. Cytometry 10: 282–293, 1989.

    Article  PubMed  Google Scholar 

  • Visser JWM, Bauman JGJ, Mulder AH, Eliason JF, Leeuw AM: Isolation of murine pluripotent hemopoietic stem cells. J. Exp. Med. 59:1576–1590, 1984.

    Article  Google Scholar 

  • Visser JWM, Van den Engh GJ: Immunofluorescence measurements by flow cytometry. In: Immunofluorescence Technology, Wick G, Traill KN, and Schauenstein K (eds). Elsevier Biomedical Press, Amsterdam, The Netherlands, 1982, pp. 95–128.

    Google Scholar 

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Author information

Authors and Affiliations

  1. New York Blood Center, 310 East 67th Street, New York, NY 10021, USA

    J. F. Keij

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  1. J. F. Keij
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Editor information

Editors and Affiliations

  1. Centre National de le Recherche Scientifique, Laboratorie de Cytométrie (UPS 47), 7, rue Guy Moquet, F-94800, Villejuif, France

    Alain Jacquamin-Sablon

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© 1996 Springer-Verlag Berlin Heidelberg

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Keij, J.F. (1996). Introduction to High-Speed Flow Sorting. In: Jacquamin-Sablon, A. (eds) Flow and Image Cytometry. NATO ASI Series, vol 95. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61115-5_16

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  • DOI: https://doi.org/10.1007/978-3-642-61115-5_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64701-7

  • Online ISBN: 978-3-642-61115-5

  • eBook Packages: Springer Book Archive

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