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Methods for Studying Renal Toxicity

  • Conference paper
Toxicology - From Cells to Man

Part of the book series: Archives of Toxicology ((TOXICOLOGY,volume 18))

Abstract

Most nephrotoxic compounds, such as drugs and industrial compounds, exert their noxious effect in a specific region of the kidney. Within the kidney, the proximal tubular cells (PTC) are exposed to the highest concentrations of xenobiotics due to their transporting, concentrating and drug metabolizing abilities. Therefore mechanisms of nephrotoxicity are best studied in these particular cells. For this purpose, well defined in vitro systems are needed. Many in vitro systems to study renal toxicity have been described, ranging from isolated perfused kidneys, kidney slices, isolated tubules, primary cultures of renal cells, kidney cell lines to isolated organelles, mainly derived from rabbit, rat and mouse; reviews of the various available systems have been published in the past (Gstraunthaler 1988,Boogaard et al. 1990). In the first part of the present paper new developments in the application of kidney slices, isolated proximal tubular cells and cell lines will be reviewed. In the second part new types of renal toxicity studies in individual living or fixed cells using video microscopy, confocal laser scan microscopy and flow cytometry will be described and discussed.

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Authors and Affiliations

  1. Division of Toxicology, Leiden Amsterdam Center for Drug Research, Sylvius Laboratories, University of Leiden, P.O.Box 9503, 2300 RA, Leiden, The Netherlands

    Marieke Kruidering, Bob van de Water & J. Fred Nagelkerke

Authors
  1. Marieke Kruidering
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  2. Bob van de Water
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  3. J. Fred Nagelkerke
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Editor information

Editors and Affiliations

  1. Interkantonale Kontrollstelle für Heilmittel, Erlachstrasse 8, CH-3000, Bern 9, Switzerland

    Jürg P. Seiler

  2. Department of Pharmacology, Charles University of Prague, 3rd Medical School, Ruská 87, Czech Republic

    Olga Kroftová

  3. Department of Pharmacology, Charles University, Faculty of Medicine, Karlovarská 48, CZ-30166, Pilsen, Czech Republic

    Vladislav Eybl

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© 1996 Springer-Verlag Berlin Heidelberg

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Kruidering, M., van de Water, B., Nagelkerke, J.F. (1996). Methods for Studying Renal Toxicity. In: Seiler, J.P., Kroftová, O., Eybl, V. (eds) Toxicology - From Cells to Man. Archives of Toxicology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61105-6_18

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  • DOI: https://doi.org/10.1007/978-3-642-61105-6_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64696-6

  • Online ISBN: 978-3-642-61105-6

  • eBook Packages: Springer Book Archive

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