Somaclonal Variation in Rauwolfia

  • V. A. Kunakh
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 36)

Abstract

Rauwolfia (Apocynaceae family) species are distributed in tropical regions of Africa, Central and South America, Australia, and Southeast Asia. Altogether, the genus covers 140 species, most of which are shrubs, less frequently trees up to 18–21 m in height (R. macrophylla Stapf and R. caffra Sond), or intermediate forms up to 6 m high (R. vomitoria Afzel).

Keywords

Biomass Sucrose Phosphorus Agar Alkaloid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Akram M, Ilahi I, Mirza MA (1990) In vitro regeneration and field transfer of Rauwolfia plants. Pak J Sci Ind Res 33: 270–274Google Scholar
  2. Antipova EA, Nikolaeva LA, Bajelidze ASh (1988) Comparative characteristics of alkaloid content and composition among some Rauwolfia L. species. Restit Resur 24: 575–578Google Scholar
  3. Butenko RG (1964)Isolated tissue culture and physiology of plant morphogenesis. Nauka, MoscowGoogle Scholar
  4. Granda LMM (1987) Informe sobre la introduction de plantas medicinales exoticas: Rauwolfia vomitoria Afzel. Rev Cubana farm 21: 187–193Google Scholar
  5. Janaki A (1962) Tetraploidy in Rauwolfia serpentina. Curr Sci 31: 520–521Google Scholar
  6. Kaukhova IE, Kunakh VA, Legeida VS, Vollosovich AG (1981a) Cytological study of highproductive cell line of Rauwolfia serpentina Benth in submerged culture. Tsitol Genet 3: 33–37Google Scholar
  7. Kaukhova IE, Vollosovich AG, Tsigankov VA (1981b) Nutrient medium selection for submerged culturing in Rauwolfia serpentina Benth tissues. Rastit Resur 17: 217–224Google Scholar
  8. Kaul MLH (1963-1964) Natural polyploidy in Rauwolfia serpentina. J Sci Res Banaras Hindu Univ 14: 100–102Google Scholar
  9. Kostenyuk I, Lubaretz O, Borisyuk N, Voronin V, Stockigt J, Gleba Y (1991a) Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family. Theor Appl Genet 82: 713–716CrossRefGoogle Scholar
  10. Kostenyuk IA, Lubaretz OF, Voronin VV, Gleba YY (1991b) Cell engineering developed for Apocynaceae plant biotechnology. Biopolymeri Kletka 7: 26–34Google Scholar
  11. Kostenyuk I A, Lubaretz OF, Endress S, Stockigt J, Gleba YY (1994) Somatic hybridization in the family Apocynaceae (Catharanthus, Rauwolfia, Rhazya, and Vinca species). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 27. Somatic hybridization in crop improvement I. Springer, Berlin Heidelberg New York, pp 405–424CrossRefGoogle Scholar
  12. Kovaleva TA, Shamina ZB, Butenko RG (1972) Effect of nitrogen mustard on isolated tissue culture of Rauwolfia. Genetika 8: 46–54Google Scholar
  13. Kunakh VA (1984) Peculiarities of structural mutagenesis in populations of cultured plant cells. In: Dubinin NP (ed) Uspekhi Sovrem Genetiki, vol 12, Nauka, Moscow, pp 30–62Google Scholar
  14. Kunakh VA (1994) Genome variability and accumulation of indoline alkaloids in Rauwolfia serpentina Benth cell culture. Biopolymeri Kletka 10: 3–30Google Scholar
  15. Kunakh VA, Alkhimova EG (1989) Rauwolfia serpentina: in vitro culture and the production of ajmaline. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 7. Medicinal and aromatic plants II. Springer, Berlin Heidelberg New York, pp 398–416Google Scholar
  16. Kunakh VA, Gubar SI (1993) Rauwolfia: strategy for generation of cell strains-producents of alkaloids. In: SkryabIn KG (ed) Plant biotechnology and molecular biology. Abst 2nd Symp, Pushchino, Russia, 382 ppGoogle Scholar
  17. Kunakh VA, Kaukhova IG, Alpatova LK, Vollosovich AG (1982) Peculiarities of cell behavior in tissue culture of Rauwolfia serpentina Benth. Tsitol Genet 5: 6–10Google Scholar
  18. Kunakh VA, Voityuk LI, Alkhimova EG, Alpatova LK (1984) Callus tissue formation and induction of organogenesis in Pisum sativum L. Fiziol Rast 31: 542–543Google Scholar
  19. Kunakh VA, Kostenyuk IA, Vollosovich AG (1986) An increase in the amount of nuclear DNA during biosynthesis of alkaloids in the culture of Rauwolfia serpentina Benth tissue. Dokl Acad Nauk Ukr SSR Ser B 7: 62–65Google Scholar
  20. Martinez PJA (1983) Distribucion de alcaloides indolicos en species del genero Rauwolfia. Boll Resen CIDA Plant Med 7: 3–58Google Scholar
  21. Mitra GC, Kaul KN (1964) In vitro culture of root and stem callus of Rauwolfia serpentina Benth for reserpine. Indian J Exp Biol 2: 49–51Google Scholar
  22. Ohta S, Yatazawa M (1979) Growth and alkaloid production in callus tissues of Rauwolfia serpentina. Agric Biol Chem 43: 2297–2303CrossRefGoogle Scholar
  23. Roja G, Benjamin BD, Heble MR, Patankar AV, Sipahimalani AT (1990) The effect of plant growth regulators and nutrient conditions on growth and alkaloid production in multiple shoot cultures of Rauwolfia serpentina. Phytother Res 4: 49–52CrossRefGoogle Scholar
  24. Singh JN, Nand K (1989) Effect of nitrogen and phosphorus on yield and quality of sarpaganha (Rauwolfia serpentina Benth). Indian Drugs 26: 461–464Google Scholar
  25. Singh M, Singh A, Singh DV (1990) Effect of length and thickness of Rauwolfia serpentina rootcuttings on plant stand and root yield. Indian Drugs 27: 483–485Google Scholar
  26. Solovyan VT, Kostenyuk I A, Kunakh VA (1987) Genome changes in Rauwolfia serpentina Benth cells cultivated in vitro. Genetika 23: 1200–1208Google Scholar
  27. Solovyan VT, Popovich VA, Kunakh VA (1989) Genome rearrangements in Crepis capillaris L (Wallr) cultured cells. Genetika 25: 1768–1775Google Scholar
  28. Solovyan VT, Zakhlenjuk OV, Kunakh VA (1990) Rauwolfia genome rearrangements during culturing in vitro. Biopolymeri Kletka 6: 103–106Google Scholar
  29. Solovyan VT, Spiridonova EV, Kunakh VA (1994a) Genome rearrangements in cell cultures of Rauwolfia serpentina: diverse pattern of genome variations. Genetika 30: 250–254Google Scholar
  30. Solovyan VT, Spiridonova EV, Kunakh VA (1994b) Genome rearrangements in cultured Rauwolfia serpentina cells. 11. Relation to interspecific genome variation. Genetika 30: 399–403Google Scholar
  31. Stockigt J (1988) Alkaloidbiosynthese in Rauwolfia. Heilmittel mit 3000-jahriger Tradition. GIT 32: 608–615Google Scholar
  32. Stockigt J, Pfitzner A, Firl J (1981) Indoline alkaloids from cell suspension cultures of Rauwolfia serpentina Benth. Plant Cell Rep 1: 36–39CrossRefGoogle Scholar
  33. Tremouillaux-Guiller J, Chenieux JC (1995) Somatic embryogenesis from leaf protoplasts of Rauwolfia vomitoria Afz. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 31. Somatic embryogenesis and synthetic seed II. Springer, Berlin Heidelberg New York, pp 357–370Google Scholar
  34. Tremouillaux-Guiller J, Andreu F, Creche J (1987) Variability in tissue cultures of Choisya ternata. Alkaloid accumulation in protoclones and aggregate clones obtained from established strains. Plant Cell Rep 6: 375–378CrossRefGoogle Scholar
  35. Upadhyay N, Batygina TB (1992) Development of plantlets from cultured tissues of Rauwolfia canescens L. In: Proc 11 Int Symp Embryol and Seed Repord, St Petersburg, pp 578-579Google Scholar
  36. Vollosovich AG (1989) Some peculiarities of alkaloid accumulation in tissue culture of Rauwolfia serpentina. In: Highlights mod Biochem. Proc 14th Int Congr Biochem (Prague, 10-15 July 1988). Utzecht, Tokyo, 1989, Vol 2, pp 1177-1182Google Scholar
  37. Vollosovich AG, Butenko RG (1970) Tissue culture of Rauwolfia serpentina as a resource of alkaloids. In: Butenko RG (ed) Culture of isolated organs, tissues and cells of plant. Nauka, Moscow, pp 253–257Google Scholar
  38. Vollosovich AG, Nikolaeva LA, Zharko GR (1972) Tissue culture of some medicinal plants from Rauwolfia genus. Rastit Resur 8: 331–338Google Scholar
  39. Vollosovich AG, Puchinina TN, Nikolaeva LA (1979) Optimization of the composition of macrosalts for a Rauwolfia serpentina Benth tissue culture. Rastit Resur 15: 516–528Google Scholar
  40. Vollosovich AG, Puchinina TN, Lisunova NA (1982) Optimization of the composition of macrosalts for a Rauwolfia tissue culture. Restit Resur 18: 239–243Google Scholar
  41. Vollosovich NE, Vollosovich AG, Kovaleva TA, Shamina ZB, Butenko RG (1976) Strains of a Rauwolfia serpentina Benth tissue culture and their productivity. Rastit Resur 12: 578–583Google Scholar
  42. Yamamoto O, Yamada Y (1986) Production of reserpine and its optimization in cultured Rauwolfia serpentina Benth cells. Plant Cell Rep 5: 50–63CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • V. A. Kunakh
    • 1
  1. 1.Institute of Molecular Biology and GeneticsNational Academy of Sciences of UkraineKievUkraine

Personalised recommendations