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Enzymes Involved in Intracellular Metabolism

  • H. Öhlinger
  • W. Von Mersi

Abstract

The catalase of aerobic organisms splits the toxic H2O2 produced from the mitochondrial electron transport and from various hydroxylation and oxygenation reactions into water and oxygen. Since aerobic organisms predominate in non-compacted and non-waterlogged soils, catalase activity was used to characterize soil microbial activities (Beck 1971). Catalase was also the first enzyme that was investigated in soils (Woods 1899, cited in Skujins 1978). While Kuprevich and Shcherbakova (1956, 1971) and Beck (1971) determined the activity via the produced oxygen, Baroccio (1958) and Johnson and Temple (1964) measured the unused H2O2. Soil catalase is characterized by its high persistence. Fresh root material distorts microbial catalase activity measurements since plants also contain catalase.

Keywords

Catalase Activity Dehydrogenase Activity Soil Enzyme Hydrogen Peroxide Solution Intracellular Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

References

  1. Baroccio A (1958) Catalase activity of soil as a biopedological index of fertility. Agrochimica 2: 243 – 25.Google Scholar
  2. Beck T (1971) Die Messung der Katalaseaktivität von Böden. Z Pflanzenernaehr Bodenkd 130: 68 – 81CrossRefGoogle Scholar
  3. Benefield CB, Howard PJA, Howard DM (1977) The estimation of dehydrogenase activity in soil. Soil Biol Biochem 9:67–70CrossRefGoogle Scholar
  4. Casida LE Jr, Klein DA, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371– 376CrossRefGoogle Scholar
  5. Curl H, Sandberg J (1961) The measurement of dehydrogenase activity in marine organisms. J Mar Res 19:123–138Google Scholar
  6. Griffiths BS (1989) Improved extraction of iodonitrotetrazolium-formazan from soil with dimethylformamide. Soil Biol Biochem 21:179–180CrossRefGoogle Scholar
  7. Johnson JL, Temple KL (1964) Some variables affecting the measurement of “catalase Activity” in soil. Soil Sci Soc Am Proc 28:207–209CrossRefGoogle Scholar
  8. Kuprevich VF, Shcherbakova TA (1956) Determination of invertase and catalase activity of soils. Vestsi Akad Navuk Belarusk SSR, Ser Biyal 2: 115 – 116Google Scholar
  9. Kuprevich VF, Shcherbakova TA (1971) Soil Enzymes. US Department of Commerce. National Technical Information Service, Springfield, VirginiaGoogle Scholar
  10. Lenhard G (1956) Die Dehydrogenaseaktivitat des Bodens als Maß für die Mikroorganismentätigkeit im Boden. Z Pflanzenernaehr Bodenkd 73: 1 – 11CrossRefGoogle Scholar
  11. Skujins J (1978) History of abiontic soil enzyme research. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 1 – 49Google Scholar
  12. Stevenson IL (1959) Dehydrogenase activity in soils. Can J Microbiol 5: 229 – 235CrossRefGoogle Scholar
  13. Tabatabai MA (1982) Soil enzymes. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, part 2. Am Soc Agron, Soil Sci Soc Am, Madison, Wiscinson, pp 903 – 947Google Scholar
  14. Thalmann A (1968) Zur Methodik der Bestimmung der Dehydrogenaseaktivität im Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtsch Forsch 21:249– 258Google Scholar
  15. Trevors JT (1984) Dehydrogenase activity in soil: a comparison between the INT and TTC assay. Soil Biol Biochem 16: 673 – 674CrossRefGoogle Scholar
  16. von Mersi W, Schinner F (1991) An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol Fertil Soils 11: 216 – 220CrossRefGoogle Scholar
  17. Woods AF (1899) The destruction of chlorophyll by oxidizing enzymes. Zentralbl Bakteriol Parasitenkd Abt 2 5: 745 – 754Google Scholar

References

  1. Beck T (1971) Die Messung der Katalaseaktivität von Böden. Z Pflanzenernaehr Bondenkd 130; 38 – 81Google Scholar
  2. Stefanic G, Beck T, Schwemmer J, Hartmann F, Varbancin A (1984) Apparatus for measuring soil catalase activity. 5th Symp Soil Biol, JASJ Buckarest: 47 – 50Google Scholar
  3. Trevors JT (1984) Rapid gaschromatographic method to measure H2O2 Oxidoreductase activity in soil. Soil Biol Biochem 16: 525 – 526CrossRefGoogle Scholar

References

  1. Glathe H, Thalmann H (1970) Über die mikrobielle Aktivät und ihre Beziehungeb zu Fruchtbarkeitsmerkmalen einger Ackerbö unter besonderer Berücksichtigung der Dehydrogenaseaktivät.2. Mitt Zentralbl Bakt II 124:24–36Google Scholar
  2. Thalmann A (1968) Zur Methodik der Bestimmung der Dehydrogenaseaktivät im Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtsch Forsch 21:249–258Google Scholar

References

  1. Mersi von W, Schinner F (1991) An improved and accurate method of determining the dehydrogenase actitvity of soil with iodonitrotetrazolium chloride. Biol Fertil Soil 11: 216 – 220CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • H. Öhlinger
  • W. Von Mersi

There are no affiliations available

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